Yinkai Xi^1,2, Biao Zeng³, Hengyu Huang^1,4,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 987-1001, 2021, DOI:10.32604/phyton.2021.014218

Abstract An efficient regeneration system of Rhynchostylis retusa was established to provide technical reference for the application of tissue culture tube seedlings in production. The mixtures of callus and protocorm from aseptic germination were used as explants. The optimal media of each stage was selected for callus proliferation, protocorm occurrence and growth, rejuvenation and rooting via a single, complete combination and orthogonal experiment. The results showed that the optimal medium for callus proliferation, protocorms occurrence and growth was 1/2 Murashige and Skoog (MS) medium adding 50 g·L⁻¹ banana puree, 0.1 mg·L⁻¹ α-naphthaleneacetic acid (NAA), 1.5 mg·L⁻¹ 6-benzylaminopurine (6-BA) and 1.0 mg·L⁻¹… More >

Tantan Zhang^1,2,#, Xiaoping He^3,#, Binglin Chen^1,#, Longxin He^1,2, Xiangru Tang^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 815-826, 2021, DOI:10.32604/phyton.2021.014168

Abstract As one of the most important nutrients for plants, potassium (K) has substantial effects on growth and development of crops. Present study was conducted in three different sites in South China in late season in 2019 with the objective to study the effects of different applied amounts of K fertilizer on yield formation and lodging of rice. Four K fertilizer treatments, K₀: 0 kg potassium oxide (K₂O) ha⁻¹ (control); K₁: 64.20 kg K₂O ha⁻¹ ; K₂: 128.55 kg K₂O ha⁻¹ and K₃ 153.90 kg K₂O ha⁻¹ were applied in the field experiment. The results showed that K₂ and K₃ treatments… More >

Lucía Isabel Chávez-Ortiz¹, José Francisco Morales-Domínguez¹, Araceli Rodríguez-Sahagún², Eugenio Pérez-Molphe-Balch^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1003-1013, 2021, DOI:10.32604/phyton.2021.012862

Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^–1 BA, obtaining a mean… More >

Liling Kang¹, Yuejun He^1,*, Lipeng Zang¹, Jianpeng Si¹, Ying Yang¹, Kaiping Shen¹, Tingting Xia¹, Qiyu Tan¹, Bangli Wu¹, Yun Guo¹, Wei Wang², Qin Liang³

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 701-717, 2021, DOI:10.32604/phyton.2021.014740

Abstract Arbuscular mycorrhizae (AM) fungi affect nutrient uptake for host plants, while it is unclear how AM fungi interacting with soil litter affect plant growth and nutrient utilization through mycorrhizal networks in karst soil of deficient nutrients beyond the rhizosphere. An experiment was conducted in a microcosm composed of a planting compartment for Cinnamomum camphora seedlings with or without Glomus mosseae fungus (M⁺ vs. M⁻ ) and an adjacent litter compartment containing or not containing additional litter material of Arthraxon hispidus (L⁺ vs. L⁻ ), where the compartments are connected either by nylon mesh of 20 μm or 0.45 μm which… More >

Lijuan Kong^1,2, Haiye Yu^1,2, Zhaojia Piao^1,2, Meichen Chen^1,2, Jingmin Dang¹, Lei Zhang^1,2, Yuanyuan Sui^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1015-1030, 2021, DOI:10.32604/phyton.2021.014190

Abstract Haze is mainly caused by the suspended particulate matters in the air, of which the particulate matters pollution harms leaf vegetables. In this paper, oilseed rapes at four different growing periods were investigated in a simulated particulate pollution environment. In combination of hyper-spectral technology and micro examination, the response of hyper-spectral characteristics of the leaf to particulate matters was investigated in-depth. The hyperspectral, chlorophyll content, net photosynthetic rate and stomatal conductance of leaf were obtained. The deposition and adsorption of particulate matters on the leaf were observed by Environmental Scanning Electron Microscope (ESEM). Normalized difference vegetation index (NDVI), modified red… More >

Lin Zhang^1,2,*, Yiting Zou¹, Zhong Bian¹, Dong Xie¹, Hadi Yeilaghi¹, Xiaolei Fan^1,2, Dongsheng Zhao^1,2, Qiaoquan Liu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 789-802, 2021, DOI:10.32604/phyton.2021.014880

Abstract Rice panicle is the sink organ where assimilation product accumulates, and its morphology determines the rice yield. Panicle length has been suggested as a yield-related trait, but the genetic factor for its control is still limited. In this study, we carried out fine-mapping of qPL8, a QTL identified for panicle length in our previous work. Near isogenic line (NIL) with qPL8 exhibited elongated panicle without obvious effect on other panicle elements. With five key recombinants from NIL population, the locus was finally narrowed down to a 278-kb region, where 44 genes are annotated. By comparing the genomic sequence of two… More >

Gang Wei^#, Hongxia Yang^#, Jingwen Wu, Zixian Xiong, Nan Wang^*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 803-813, 2021, DOI:10.32604/phyton.2021.014505

Abstract There is a positive correlation between fertility and yield, and the decrease of fertility is bound to a greatly reduced crop yield. Male sterile mutants can be used in hybrid rice. Therefore, rice male sterility has an important value in research and application, and the study of related mutants is also very vital. The mutant ms10 (male sterile 10) reported in this study was induced by ethyl methane sulfonate (EMS) in the indica maintainer line Xinong 1B. There was no significant difference between the ms10 and wild type in the vegetative growth stage. However, in the reproductive growth stage, ms10… More >

Shahida Akter, Israt Jahan, Md. Amir Hossain^*, Mohammad Anwar Hossain^*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 949-970, 2021, DOI:10.32604/phyton.2021.014411

Abstract Drought susceptibility and low genetic variability are the major constraints of lentil (Lens culinaris Medik.) production worldwide. Development of an efficient pre-field drought phenotyping technique and identification of diversified drought tolerant lentil genotype(s) are therefore vital and necessary. Two separate experiments were conducted using thirty diverse lentil genotypes to isolate drought tolerant genotype(s) as well as to assess their diversity. In both of the experiments, significant (p ≤ 0.01) variation in genotype (G), treatment (T) and G X T was observed for most of the studied traits. In experiment I, genotypes were examined for drought tolerance at the seedlings stage… More >

Shiyang Li^1,#, Xue-Ao Liu^2,#, Lizi Zhao², Huiqing Huang³, Bei Li², Zhizhong Song², Meixia Liang², Hongxia Zhang^2,3, Limin Wang^2,*, Shenglin Zhou^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 761-772, 2021, DOI:10.32604/phyton.2021.016314

Abstract Seven in absentia (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a seven in absentia gene IbSINA5 was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that IbSINA5 was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm and nucleus.… More >

Abdulkhaliq Alshoaibi^*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 731-745, 2021, DOI:10.32604/phyton.2021.015476

Abstract Seeds of three Saudi pearl millet cultivars (Pennisetum spicatum) from three regions (Madinah, Khulais and Jaizan) were inoculated with arbuscular mycorrhizal fungus Glomus mosseae obtained from the Agriculture Research Center of Giza, Egypt to enhance their salt tolerance. Five different NaCl concentrations (0, 30, 60, 90, and 120 mM) were used for treating cultivars with and without mycorrhiza. Growth rates, chlorophyll content, chlorophyll fluorescence (Fv/Fm), proline content and gas exchange were measured to determine the effect of salinity on these cultivars. The results indicated that compared to cultivars without mycorrhiza, all cultivars with mycorrhiza had enhanced growth and physiological parameters… More >