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  • Open Access

    ARTICLE

    Comparative Analysis of the Complete Chloroplast Genome Sequences of Four Origin Plants of Lonicerae Flos (Lonicera; Caprifoliaceae)

    Sisi Liu1, Lisi Zhou2, Jiaoli Huang1, Huijie Zeng1, Zhongquan Qiao1, Yongxin Li1,*, Gang Zhang3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1503-1516, 2022, DOI:10.32604/phyton.2022.020390

    Abstract Lonicerae Flos (LF) derived from the dried flower buds or opening flowers of four Lonicera plants (Lonicera macranthoides, L. hypoglauca, L. confusa, and L. fulvotnetosa), is a popular traditional Chinese medicine. Because the four origin plants are very similar in morphology, it is difficult to control the quality of LF in actual production. Over the past decade, many reports have pointed out the differences among them, including the botanical characteristics and active ingredients. However, there is still a lack of rapid methods that can be applied to the identification of the four origins. In this study, comparative analysis of the… More >

  • Open Access

    ARTICLE

    Effects of Rate and Source Organo-Mineral Material on Forage Yield and Nutritive Value of Barley-Pea Mixed under Arid Conditions

    Walid Soufan*, Nasser A. Al-Suhaibani

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1483-1493, 2022, DOI:10.32604/phyton.2022.020264

    Abstract Cereal-legume intercropping plays a vital role in the subsistence food production system that prevails in the arid regions. It not only provides profitable crop productivity for agricultural communities but also plays an important role in improving soil fertility. Therefore, the present research was conducted to assess the effect of the organic and mineral fertilizers on the forage yield and nutritional value in barley-pea intercropping system. The results revealed that the quality of forage grass is significantly influenced by both organic and inorganic fertilizer. Thus, organic fertilizer application has significantly influenced the dry matter (DM), crude ash (CA), crude protein (CP),… More >

  • Open Access

    ARTICLE

    Bioinformatics Analysis of Disease Resistance Gene PR1 and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials

    Huimin Cui, Shuo Qu, Abraham Lamboro, Yaolei Jiao, Piwu Wang*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1445-1464, 2022, DOI:10.32604/phyton.2022.020010

    Abstract In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering, and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube method. In CryAb-8Like transgenic high-generation T7 receptor soybean, a new material that is resistant to insects and diseases is obtained. For T2 transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed… More >

  • Open Access

    ARTICLE

    Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid, Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

    Longhua Zhou1,2,#, Ting He1,2,#, Jing Li1,2,3, Guimei Guo1,2, Yingbo Li1,2, Hongwei Xu1,2, Runhong Gao1,2, Linli Huang1,2, Yifei Wang1,2, Ruiju Lu1,2, Zhiwei Chen1,2,*, Chenghong Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

    Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

  • Open Access

    REVIEW

    Formation Mechanism and Occurrence Law of Pod Shattering in Soybean: A Review

    Jinfeng Liu1, Yuzhe Zhang1, Yushi Jiang1, Hongji Sun1, Ruijie Duan1, Jing Qu2, Dan Yao1, Siyan Liu2,*, Shuyan Guan2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1327-1340, 2022, DOI:10.32604/phyton.2022.019870

    Abstract Seed shattering refers to the phenomenon in which the pods split along the abdominal and back sutures before the crop is received, so that the seeds are spread. Seed shattering is vital to the reproduction of their offspring in wild plants, but it is also the main cause of crop yield loss reason. Pod-explosion resistance is a complex process of physical and physiological and biochemical reactions. Soybean seed shattering phenomenon is widespread, which severely restricts the development of soybean industry. Seed shattering (pod cracking or fruit dropping) is essential for the reproduction of its offspring in wild plants, but it… More >

  • Open Access

    ARTICLE

    Discovering Candidate Chromosomal Regions Linked to Kernel Size-Related Traits via QTL Mapping and Bulked Sample Analysis in Maize

    Hameed Gul1, Mengya Qian1, Mohammad G. Arabzai1,2, Tianhui Huang1, Qiannan Ma1, Fangyu Xing1, Wan Cao1, Tingting Liu1, Hong Duan1, Qianlin Xiao1,*, Zhizhai Liu1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1429-1443, 2022, DOI:10.32604/phyton.2022.019842

    Abstract Kernel size-related traits, including kernel length, kernel width, and kernel thickness, are critical components in determining yield and kernel quality in maize (Zea mays L.). Dissecting the phenotypic characteristics of these traits, and discovering the candidate chromosomal regions for these traits, are of potential importance for maize yield and quality improvement. In this study, a total of 139 F2:3 family lines derived from EHel and B73, a distinct line with extremely low ear height (EHel), was used for phenotyping and QTL mapping of three kernel size-related traits, including 10-kernel length (KL), 10-kernel width (KWid), and 10-kernel thickness (KT). The results… More >

  • Open Access

    ARTICLE

    Effects of Chemical Products on Fire Blight Suppression, and Fruit Production and Quality in Apple (Malus domestica Borkh.) cv. Golden Glory

    Álvaro Rodríguez-Peña1, Ana C. Gonzalez-Franco1, Jared Hernández-Huerta1, Nora A. Salas-Salazar1, Dámaris L. Ojeda-Barrios1, Esteban Sánchez2, Loreto Robles-Hernández1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1341-1351, 2022, DOI:10.32604/phyton.2022.019728

    Abstract Antibiotics are widely used in fire blight management programs, yet there are no studies that demonstrate the evaluation of their efficacy in Mexico. Therefore, the present study was conducted to investigate the effects of the active ingredients in five commercial products (Kasumin® 2L, Agrygent Plus®, Agricultural Terramycin®, Agrimicin® 100, and Actigard®) on fire blight suppression, and fruit yield and quality of apple (Malus domestica Borkh.) cv. Golden Glory. The experiment was conducted in a commercial orchard using a completely randomized block design, with six treatments: (1) Oxytetracycline [Ox], 110 mg L−1; (2) Kasugamycin [Kas], 4.7 mL L−1; (3) Oxytetracycline + Gentamicin [Ox… More >

  • Open Access

    ARTICLE

    Comparative Transcriptome Analysis Reveals Different Mechanisms of Adaptation to Environment among Three Species of Saussurea DC.

    Weimin Zhao1, Fengzhen Li1, Guomin Shi3, Jialei Guo1, Guifang He1, Tao He2,3,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1517-1528, 2022, DOI:10.32604/phyton.2022.019630

    Abstract Saussurea medusa, Saussurea hypsipeta and Saussurea obvallata are typical alpine snowline plants growing in the Qinghai-Tibet plateau. They are characterized by a specialized morphology. S. medusa and S. hypsipeta have very dense trichomes on whole plant, whereas S. obvallata has transparent bracts covered inflorescences. The different forms reflect their adaptation to cold environments. To investigate the different mechanisms of adaptation of these species to cold temperatures, transcriptome sequencing was performed in three species of Saussurea DC. A total of 116394 137237 and 113879 Unigenes were identified from S. medusa, S. hypsipeta and S. obvallata, respectively. Of these, 55909 (48.03%), 65519… More >

  • Open Access

    ARTICLE

    Organic Materials Could Improve the Phytoremediation Efficiency of Soil Potentially Hazardous Metal by Sedum alfredii Hance

    Peifan Wang1,#, Shengting Rao1,#, Jia Fang2, Yongqiang Lv2, Anying Zhao2, Zhengqian Ye2, Weijun Fu1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1529-1542, 2022, DOI:10.32604/phyton.2022.019368

    Abstract Soil potentially hazardous metal (PHM) is continually attracting public attention worldwide, due to its highly toxic properties and potentially huge damage to human being through food chain. Phytoremediation is an effective and eco-friendly way in remediation technology. A pot experiment was carried out to investigate the effect of different organic materials (biogas residue (BR), mushroom residue (MR), and bamboo-shoot shell (BS)) application on phytoremediation of two PHM-contaminated soils (Fuyang soil as ‘heavily-polluted soil’ and Wenzhou soil as ‘moderately-polluted soil’, respectively) by Sedum alfrecdii Hance. The results indicated: 1) for moderately-polluted soil, the 5% BR treatment had the strongest activation to… More >

  • Open Access

    ARTICLE

    Cloning of the Soybean sHSP26 Gene and Analysis of Its Drought Resistance

    Siyan Liu1,*, Jinfeng Liu1, Yuzhe Zhang1, Yushi Jiang1, Shaowang Hu1, Andi Shi1, Qiyao Cong1, Shuyan Guan2,*, Jing Qu2, Yao Dan1

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1465-1482, 2022, DOI:10.32604/phyton.2022.018836

    Abstract Exploring the molecular mechanism of soybean response to drought stress, providing a basis for genetic improvement and breeding of heat-resistant varieties, relying on the transcriptome sequencing data of unpollinated ovary at the seven-leaf stage of soybean Jinong 18(JN18) and Jinong 18 mutant (JB18) soybeans, using reverse transcription, one gene in the sHSP family was cloned using PCR (RT-PCR) and it was named sHSP26. In this experiment, the soybean sHSP26 gene was successfully cloned by RT-PCR, the protein encoded by the sHSP26 gene was analyzed by bioinformatics, and the sHSP26 gene overexpression vector and CRISPR/Cas9 gene-editing vector were constructed. The positive… More >

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