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  • Formononetin Inhibits Non-Small Cell Lung Cancer Proliferation via Regulation of mir-27a-3p through p53 Pathway
  • Abstract Objective: The aim of the present study was to investigate the anti-tumor effects of formononetin on human nonsmall cell lung cancer (NSCLC) and its potential molecular mechanism. Methods: A549 cells were treated with different concentrations of formononetin, then detected the cell proliferation, apoptosis and the expression of HIPK2 respectively by MTT assay, flow cytometry analysis and RT-qPCR. Then the interaction between miR- 27a-3p and its target gene HIPK2 was verified through luciferase reporter assay. The expression of miR-27a- 3p, HIPK2, and p53 was detected after being treated with different formononetin concentrations by RT-qPCR and western blot. Results: The results showed…
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  • Mir-612 Inhibits Proliferation and Invasion of Urothelial Carcinoma of Bladder Cells through Activating Hippo Pathway via Targeting PMEPA1
  • Abstract Urothelial carcinoma of bladder (UCB) is a common urological malignancy in the world, but its progression mechanism remains unclear. MiR-612 was found as an anti-tumor factor in multiple types of cancer, while few studies have revealed its functions in UCB cells. Based on this, UCB cells such as HTB-9 and HTB-4, and normal urothelial of bladder cells such as SV-Huc1, were used as subjects in this study. Western blot, qRTPCR, CCK-8 assay and transwell assay were used to assess functions of miR-612 in UCB cells. The database, miRWalk, was used to search for potential targets of miR-612, and dual-luciferase reporter…
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  • HBx Downregulates miR-422a Expression via Activation of FOXG1/Q1/E1 in HepG2 Cells
  • Abstract microRNA-422a (miR-422a) is downregulated in many hematopoietic tumors and solid tumors including hepatocellular carcinoma. We previously demonstrated that hepatitis B virus X protein (HBx) downregulated expression of miR-422a in HCC cell line HepG2 in vitro. However, we explore the mechanisms underlying this action. Forkhead box proteins (FOX) G1/Q1/E1 are known to negatively regulate miR-422a expression, and this prompted us to determine whether HBx suppresses miR-422a expression via activation of FOXG1/Q1/E1. The relationship between FOXG1/Q1/E1 and miR-422a in HepG2 cells stably expressing HBx was assessed with qRT-PCR. The correlation between HBx and FOXG1/Q1/E1 was determined with qRT-PCR and western blot in…
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  • A Nomogram for Predicting Lateral Lymph Node Metastasis in Cases of Papillary Thyroid Micro-Carcinoma with Suspected Lymph Node Metastasis
  • Abstract The elevation for lateral lymph node metastasis (LLNM) plays an important role in therapeutic decision-making for thyroid carcinoma. A reliable forecasting model for LLNM in patients with papillary thyroid micro-carcinoma (PTMC) is needed, using clinicopathological characteristics. A total of 576 PTMC patients with suspicious lateral cervical lymph node (II, III, IV or V region) metastasis and known clinicopathological variables were randomly collected at Shenzhen Second People’s Hospital. Cervical lymph node status of every patient was assessed by ultrasonography (US). The patients in this cohort study underwent thyroidectomy and lateral neck lymph node dissection. Univariate analysis and logistic regression analysis were…
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  • Effect of Cluster Nursing Mode Combined with Blood Pressure Regulation on Surgical Tolerance of Patients with Esophageal Cancer and Hypertension
  • Abstract Objective: To explore the effect of the cluster nursing mode combined with blood pressure regulation on the surgical tolerance of patients with esophageal cancer and hypertension. Methods: The clinical data of 86 patients with esophageal cancer and hypertension treated in our hospital (February 2016–February 2017) were retrospectively analyzed. The patients were randomly split into research group and reference group, with 43 cases in each group. The reference group received routine nursing, while the research group received cluster nursing combined with blood pressure regulation. The SBP, DBP and heart rates at D1 (at admission), D2 (2 h before surgery) and D3…
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  • Alteration of Ornithine Metabolic Pathway in Colon Cancer and Multivariate Data Modelling for Cancer Diagnosis
  • Abstract It is noteworthy that colon cancer is the fourth place in new cases and the fifth in mortalities according to global cancer statistics 2018. Tumorigenesis displays specific correlation with metabolic alterations. A variety of metabolites, including ornithine (Orn), are related to colon cancer according to sources of disease metabolic information retrieval in human metabolome database. The metabolic regulation of Orn pathway is a key link in the survival of cancer cells. In this study, the plasma Orn levels in colon cancer patients and healthy participants were measured by liquid chromatography tandem mass spectrometry, and the metabolic disturbances of Orn in…
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  • Growth and Invasion of 3D Spheroid Tumor of HeLa and CasKi Cervical Cancer Cells
  • Abstract Spheroids are generally self-assembled cells with the ability to generate their extracellular matrix, including the complex cell-matrix and the cell-cell interactions that resemble the functional characteristics of the corresponding tissue in vivo. The study aimed to develop a three-dimensional (3D) spheroid system for the cervical cancer cell lines, HeLa (HPV18), CaSki (HPV16), SiHa (HPV16), C33A (non-HPV), HT3 (non-HPV) as well as to identify its biological activity in the extracellular form. For the formation of the cervical cancer spheroids, the liquid overlay approach was applied, followed by embedding to the bovine collagen I matrix. Spheroid formation using the liquid overlay approach…
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