Shaoyi Ruan^1,2,3,#, Haowen Luo^1,2,3,#, Xiaoyan Zeng^1,2,3,#, Runhao Wen^1,2,3, Feida Wu^1,2,3, Xiangru Tang^1,2.*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1273-1283, 2021, DOI:10.32604/phyton.2021.015610

Abstract Vermicompost is an organic fertilizer contains multiple nutrient elements. However, the application of vermicompost in fragrant rice production is rarely reported. In order to study the effects of vermicompost application on fragrant rice (Oryza sativa L.) seedling performances, present study was conducted with two fragrant rice cultivars and four vermicompost rate treatments (0 (CK), 2.5 (Wo1), 5.0 (Wo2) and 10.0 (Wo3) g kg⁻¹ ). The results showed that vermicompost treatments significantly increased dry weight of fragrant rice seedling by 8.31–32.56% compared with CK. 21.10–59.13% higher net photosynthetic rates and 10.66–59.16% higher chlorophyll contents (chlorophyll a, chlorophyll b and total chlorophyll)… More >

Md. Ashraful Alam¹, Milan Skalicky², Muhammad Rezaul Kabir¹, Md. Monwar Hossain¹, Md. Abdul Hakim¹, Md. Siddikun Nabi Mandal¹, Rabiul Islam³, Md. Babul Anwar³, Akbar Hossain^1,*, Fahmy Hassan⁴, Amaal Mohammadein⁴, Muhammad Aamir Iqbal⁵, Marian Brestic^2,6, Mohammad Anwar Hossain⁷, Khalid Rehman Hakeem⁸, Ayman EL Sabagh^9,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1301-1320, 2021, DOI:10.32604/phyton.2021.016015

Abstract Globally among biotic stresses, diseases like blight, rust and blast constitute prime constraints for reducing wheat productivity especially in Bangladesh. For sustainable productivity, the development of disease-resistant lines and high yielding varieties is vital and necessary. This study was conducted using 122 advanced breeding lines of wheat including 21 varieties developed by Bangladesh Wheat and Maize Research Institute (BAMRI) with aims to identify genotypes having high yield potential and resistance to leaf blight, leaf rust and blast diseases. These genotypes were evaluated for resistance against leaf blight and leaf rust at Dinajpur and wheat blast at Jashore under field condition.… More >

Guoli Deng, Songbei Ying, Sasa Jing, Jun Zhou, Shiyan Lu, Ping Li^*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1089-1101, 2021, DOI:10.32604/phyton.2021.016217

Abstract Ambient temperature induces the hypocotyl elongation of seedling, called as thermomorphogenesis. It has been reported that the bHLH transcriptional factor PIF7 acts as the critical component to modulate plant thermomorphogenesis, but the underlying mechanism remains elusive. The phytohormone abscisic acid (ABA) suppresses the hypocotyl elongation under high temperature (HT) stress. As the ABI5 binding protein, AFP2 acts as the negative factor to control ABA signaling. In this study, we first identified AFP2 as the interaction protein of PIF7 in vitro and in vivo. Phenotype analysis revealed that overexpressing AFP2 reduced the hypocotyl elongation, while loss-of-function afp2 mutant showed longer hypocotyl… More >

Wenjie Wei, Yulan Hu, Wenjuan Yang, Xiaoli Li, Jiali Wei, Xiangyang Hu, Ping Li^*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1075-1087, 2021, DOI:10.32604/phyton.2021.016134

Abstract Seed germination or dormancy status is strictly controlled by endogenous phytohormone and exogenous environment signals. Abscisic acid (ABA) is the important phytohormone to suppress seed germination. Ambient high temperature (HT) also suppressed seed germination, or called as secondary seed dormancy, through upregulating ABI5, the essential component of ABA signal pathway. Previous result shows that appropriate nitric oxide (NO) breaks seed dormancy through triggering S-nitrosoglutathion reductase (GSNOR1)-dependent S-nitrosylation modification of ABI5 protein, subsequently inducing the degradation of ABI5. Here we found that HT induced the degradation of GSNOR1 protein and reduced its activity, thus accumulated more reactive nitrogen species (RNS) to… More >

Stefan M. Kolašinac¹, Zora P. Dajić Stevanović^1,*, Sofija N. Kilibarda¹,  Aleksandar Ž. Kostić^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1041-1062, 2021, DOI:10.32604/phyton.2021.015996

Abstract Carotenoids represent a large group of mainly red, orange, and yellow natural metabolites mainly involved in regulation of many metabolic processes. Carotenoids are beneficial for human health. Current study describes the importance, chemical composition and functioning of carotenoids. It is well known that carotenoids support pigments acting in light absorbance mechanisms during photosynthesis, and are known to protect the chlorophyll molecules from oxidative stress and reactive oxygen species (ROS) damage. Carotenoids are involved in signaling processes in plants, responses to environmental stresses, pollination, germination and reproduction, and development regulation. As nutrients of strong antioxidant activity that is primarily linked to… More >

Min Yan¹, Han Wang¹, Shaohua Liu¹, Ye Xu¹, Zizhuo Nie¹, Yifeng Zhou², Li Li^2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1293-1300, 2021, DOI:10.32604/phyton.2021.015601

Abstract Rho-related GTPase from plants (ROP) proteins play an essential role in plant stress resistance. In this study, the full-length GlROP6 gene was cloned based on G. littoralis transcriptome sequencing data acquired in response to salt stress. The protein sequence, conserved domains, secondary structure, three-dimensional structure, phylogenetic relationships, and expression pattern of the GlROP6 gene were systematically analysed. Our results showed that the full-length GlROP6 gene had an open reading frame of 606 bp, which encoded 201 amino acid residues with a relative molecular weight of 22.23463 kDa and a theoretical isoelectric point of 9.06. Amino acid sequence analyses indicated that… More >

Linjun Cai^1,#, Junhua Liu^2,#, Han Yun¹, Dan Du¹, Xiaolong Zhong¹, Zhenlin Yang¹, Xianchun Sang¹, Changwei Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1103-1117, 2021, DOI:10.32604/phyton.2021.015532

Abstract Leaf color mutants are ideal materials for studying many plant physiological and metabolic processes such as photosynthesis, photomorphogenesis, hormone physiology and disease resistance. In this study, the genetically stable yellow-green leaf mutant ygl16 was identified from mutated “Xinong 1B”. Compared with the wild type, the pigment concentration and photosynthetic capacity of the ygl16 decreased significantly. The ultrastructural observation showed that the distribution of thylakoid lamellae was irregular in ygl16 chloroplasts, and the grana and matrix lamellae were blurred and loose in varied degrees, and the chloroplast structure was disordered, while the osmiophilic corpuscles increased. The results of the genetic analysis… More >

Shuo Qu¹, Yaolei Jiao², Lamboro Abraham², Piwu Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1177-1192, 2021, DOI:10.32604/phyton.2021.015206

Abstract The fatty acid dehydrogenase gene plays an important role in regulating the oleic acid content in soybean. Genome-wide association study screened out soybean oleic acid related gene Gm15G117700. A fragment size of 693bp was obtained by PCR amplification of the gene and, it was connected by seamless cloning technology to the pMD18T cloning vector. Based on the gene sequence cloned, bioinformatic analysis of gene protein was performed. The overexpression vector of Gm15G117700 and the CRISPR/Cas9 gene editing vector were constructed. The positive plants were obtained by Agrobacterium-mediated transformation of soybean cotyledon nodes and T₂ plants were identified by conventional PCR,… More >

Menghua Xiao¹, Yuanyuan Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1131-1146, 2021, DOI:10.32604/phyton.2021.015093

Abstract The flooding caused by heavy rainfall in rice irrigation area and the drought caused by the drop of groundwater level are the research focus in the field of irrigation and drainage. Based on the comparative experiment and farmland water level control technology, this paper studied the average soil temperature under different soil layers (TM), the daily temperature change (TDC), the photosynthetic accumulation of single leaf and canopy in rice, and response of photothermal energy to rice root characteristics and growth factors in the paddy field under drought conditions. The results showed that the peak soil temperature under drought treatment was… More >

Simei Yao^1,2, Yu Jin^2,3, Limin Zhang², Ningfei Lei^1,*, Wei Xue^2,*, Jinsong Chen³, Qian Zhang², Feihai Yu^1,2

Phyton-International Journal of Experimental Botany, Vol.90, No.4, pp. 1259-1271, 2021, DOI:10.32604/phyton.2021.014968

Abstract Soil nutrients are commonly heterogeneously distributed and earthworms are one of the most common soil organisms. While effects of both soil nutrient heterogeneity and earthworms have been well studied, their interactive effect on plant community productivity has rarely been tested. In a greenhouse experiment, we constructed experimental plant communities by sowing seed mixtures of four grasses, two legumes and two forbs in either a heterogeneous soil consisting of low and high nutrient soil patches or a homogeneous soil where the low and high nutrient soil patches were evenly mixed. The earthworm Eisenia fetida was either added to these soils or… More >