TING LAN, SHENG QIN, XIAOJIN GONG, PING ZHENG, JIAXIN YAN, YANG LIU^*

BIOCELL, Vol.45, No.5, pp. 1313-1319, 2021, DOI:10.32604/biocell.2021.015923

Abstract Invasive stratified mucin-producing carcinoma (ISMC) is a recently described histologic variant of high-risk human papillomavirus (HPV)-associated endocervical adenocarcinoma, as the putative invasive counterpart of the stratified mucin-producing intraepithelial lesion (SMILE). ISMC can display variable architectural patterns and usually coexists with other more conventional types of HPV-associated carcinomas, which makes diagnosis and differential diagnosis of ISMC is difficult for pathologists. Moreover, the prognosis of ISMC is still controversial. We analyzed 6 ISMCs with detailed pathological and clinical information. Intraepithelial lesion, including 1 high-grade squamous intraepithelial lesion and 1 SMILE, was found. Various architectures were observed (including nest, glandular, solid, trabecular, and… More >

SULAIMAN A. AL YOUSEF^*

BIOCELL, Vol.45, No.5, pp. 1403-1411, 2021, DOI:10.32604/biocell.2021.015450

Abstract Seeds from soybean collected from different commercial markets were surveyed for seed-borne fungi. Ninetyeight fungal colonies were isolated all over three monthly isolations constituting twenty-two fungal species. The isolated fungi were belonging to the genera: Alternaria, Aspergillus, Cephalosporium, Chaetomium, Cladosporium, Colletotrichum, Curvularia, Fusarium, Macrophomina, Mucor, Penicillium, Rhizoctonia, Rhizopus, Sclerotium, Stemphylium, and Verticillium. Assay of the antifungal activity of four ethanolic extracts of clove, cinnamon, garlic, and mint was carried out against the most common fungal species (Aspergillus flavus, A. niger, Fusarium solani, F. oxysporium, and F. moniliforme), which significantly reduced the growth of tested fungi. Clove extract recorded the highest… More >

GAGANJOT GUPTA¹, DEEPAK BANSAL², ANSHULA SHARMA¹, TAWSEEF AHMAD¹, ATUL SACHDEV², AJAZ AHMAD³, HAMED A. EL-SEREHY⁴, BALJINDER KAUR^1,*

BIOCELL, Vol.45, No.5, pp. 1299-1311, 2021, DOI:10.32604/biocell.2021.015411

Abstract Helicobacter pylori infection has been significantly linked to Peptic Ulcer Disease and Gastric Cancer. Metabolomic fingerprinting may offer a principal way of early diagnosis and to understand the molecular mechanism of H. pylori-induced pathogenicity. The rationale of the study is to explore the underlying distinct metabolic mechanisms of H. pylori-induced PUD and to identify potential biomarkers for disease diagnosis and associated risks using Gas chromatography/mass spectrometry. GC/MS-based analytical method was used to compare metabolic profiles of healthy controls (N = 20) and peptic ulcer patients (N = 45). Acquired metabolomic data were analyzed by constructing a diagnostic model using principal… More >

ATTALLA F. EL-KOTT^1,2,*, AYMAN E. EL-KENAWY³, EMAN R. ELBEALY⁴, ALI S. ALSHEHRI¹, HEBA S. KHALIFA², MASHAEL MOHAMMED BIN-MEFERIJ⁵, EHAB E. MASSOUD^6,7,8, AMIRA M. ALRAMLAWY⁹

BIOCELL, Vol.45, No.5, pp. 1337-1353, 2021, DOI:10.32604/biocell.2021.015464

Abstract This study examined if the anti-tumorigenesis effect of Exendin-4 in HT29 and HCT116 colorectal cancer (CRC) involves modulation of SIRT1 and Akt/GSR3K/β-catenin/NF-κB axis. HT29 and HCT116 cells were treated either with increasing levels of Exendin-4 (0.0-200 µM) or with Exendin-4 (at its IC₅₀) in the presence or absence of EX-527 (10 µM/a selective SIRT1 inhibitor) or Exendin-4 (9-39) amide (E (9-39) A) (1 µM/an Exendin-4 antagonist). In a dose-dependent manner, Exendin-4 inhibited cell survival, but enhanced levels of lactate dehydrogenase (LDH) and single-stranded DNA (ssDNA) in both HT29 and HCT116. In both cell lines and at it has an IC₅₀… More >

BARKAT ALI KHAN¹, FAZAL KARIM¹, MUHAMMAD KHALID KHAN^1,*, FAHEEM HAIDER¹, SADIQULLAH KHAN²

BIOCELL, Vol.45, No.5, pp. 1273-1285, 2021, DOI:10.32604/biocell.2021.015323

Abstract The fourth most predominant overwhelming type of trauma is burn injuries worldwide. Ideal wound healing dressings help in the wound healing process in a lower time with less pain. Commonly used dry wound dressing, like absorbent gauze or absorbent cotton, possess limited therapeutic effects and require repeated use, which further exaggerates patients’ suffering. In contrast, hydrogels films present a promising alternative to improve healing by guaranteeing a moisture balance at the wound site. The aim of the current study was to synthesize Tamarix aphylla (T. aphylla) extract-loaded hydrogel film with Na-CMC and pectin and to study their wound healing properties.… More >

MOHAMMAD EL-METWALLY^1,*, MAMDOUH ABDEL-MOGIB², MANAL ELFEDAWY², GAAD SOHSAH², AHMED REZK³, MAHMOUD MOUSTAFA⁴, MOHAMED SHAABAN^5,6

BIOCELL, Vol.45, No.5, pp. 1263-1271, 2021, DOI:10.32604/biocell.2021.013198

Abstract Microorganisms are a huge mine of bioactive metabolites, and actinomycetes are one of the very active groups in this area. In this article, we are concerned about the full taxonomical characterization of Streptomyces lividans AM, isolated from Egyptian soil. This isolate produced three new bioactive metabolites, namely: 1-Nona-decanoyl,4-oleyl disuccinate (1), filoboletic acid; (9Z,11E)-8,13-dihydroxy octadeca-9,11-dienoic acid (2), and sitosteryl-3β-D-glucoside (3). Extensive 1D and 2D NMR and HR-mass spectrometry were used to elucidate the structures of the three compounds. Moreover, ten known compounds were also identified. The antimicrobial activity of the producing organism and newly reported compounds (1–3) was investigated against a… More >

NARAYANAN NAMPOOTHIRI V. P.¹, VIGNESH SUNDARARAJAN¹, PALLAVI DAN¹, G. DEVANAND VENKATASUBBU^2,*, SAHABUDEEN SHEIK MOHIDEEN^1,*

BIOCELL, Vol.45, No.5, pp. 1251-1262, 2021, DOI:10.32604/biocell.2021.015090

Abstract Alzheimer’s disease (AD) is one of the most common forms of dementia. Cognitive dysfunction and memory loss are the two main clinical symptoms of AD. Drosophila melanogaster models of AD, which are based on overexpression of human amyloid β (Aβ) or human tau (hTau) protein, have been used to study the mechanism underlying AD and to screen potential therapeutic compounds. Drugs that are currently available for AD provide only symptomatic relief. Huge unmet medical needs exists to slow, stop, or reverse the progression of AD. Thymoquinone (TQ) is an active ingredient isolated from Nigella sativa (NS) and possesses various pharmacological… More >

HAZAEL RAMIRO CEJA-GALVEZ¹, ERANDIS DHENI TORRES-SÁNCHEZ¹, JUAN HERIBERTO TORRES-JASSO², EMMANUEL REYESURIBE¹, JOEL SALAZAR-FLORES^1,*

BIOCELL, Vol.45, No.5, pp. 1241-1250, 2021, DOI:10.32604/biocell.2021.015771

Abstract Paraoxonase-1 (PON-1) is an enzyme that hydrolyzes organophosphate pesticides. The presence of polymorphisms in PON-1 (L55M and Q192R) decreases its enzyme activity and increases the risk of central nervous system (CNS) toxicity in occupationally exposed farmers, leading to chronic degenerative diseases and death. We studied 103 farmers in the region of Cienega Jalisco, Mexico, which were exposed mainly to organophosphate pesticides. We used serum and plasma samples to assay PON-1 activity and perform polymorphism analysis (L55M and Q192R) using qPCR and TaqMan probes, respectively. For both polymorphisms, there was high percentage of heterozygosity (55 LL = 0.19, LM = 0.75,… More >

CHEN-LIANG TSAI¹, YU-HUEI LIN², CHIH-YING CHANGCHIEN³, CHIH-FENG CHIAN^1,#,*, CHI-HUEI CHIANG^4,#,*

BIOCELL, Vol.45, No.5, pp. 1201-1211, 2021, DOI:10.32604/biocell.2021.014279

Abstract Primary lung graft dysfunction could significantly attribute to ischemia-reperfusion lung injury (IRLI) during transplantation surgery. β2-adrenergic agonists were one of the bronchodilators that had been well-established in the management of asthma and chronic obstructive pulmonary disease (COPD) with anti-inflammatory potency. By applying the model of isolated rat lung, we evaluated the efficacy of short-acting β2-agonist inhalation to ameliorate ischemia-reperfusion damage. The experiment protocol was 180 min of global ischemia and then reperfusion for 60 min. In the β2-agonist inhalation group, aerosolized albuterol was administrated prior ischemia procedure. Increased weight ratios of wet to dry lung and microvascular permeability were characterized… More >

JOE R. DELANEY^*

BIOCELL, Vol.45, No.5, pp. 1167-1170, 2021, DOI:10.32604/biocell.2021.017296

Abstract Single-cell sequencing data has transformed the understanding of biological heterogeneity. While many flavors of single-cell sequencing have been developed, single-cell RNA sequencing (scRNA-seq) is currently the most prolific form in published literature. Bioinformatic analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene counts. One biologically relevant variable is readily inferred from scRNA-seq gene count tables regardless of individual gene representation within single cells: aneuploidy. Since hundreds of genes are present on chromosome arms, high-quality inferences of aneuploidy can be made… More >