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  • Enhanced osteogenic differentiation of human periodontal ligament stem cells by suberoylanilide hydroxamic acid
  • Abstract Periodontitis is a type of chronic inflammation in the gingival tissue caused by infectious bacteria colonizing the surface of the teeth, leading to the destruction of tooth-supporting tissues and loss of alveolar bone. Suberoylanilide hydroxamic acid (SAHA), a class of histone deacetylase (HDAC) inhibitor, has the potential to stimulate osteoblast differentiation by acetylating histone proteins, and thus suppressing the expression of adipogenic and chondrogenic genes. However, the effect of SAHA on the differentiation of human periodontal ligament stem cells (hPDLSCs) is yet to be elucidated. Herein, we investigated the effects of SAHA on in vitro proliferation and differentiation of hPDLSCs…
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  • Construction of castor functional markers fingerprint and analysis of genetic diversity
  • Abstract In order to provide a molecular basis for selecting good hybrid combinations for the identification of castor bean germplasm resources, fingerprint and genetic diversity analysis of 52 castor bean materials from 12 regions in 5 countries were constructed by using the Functinal Markers (FMs) associated with fatty acid metabolism-related genes. A total of 72 alleles were amplified by 29 pairs of FMs with an average of 2.483 per marker and the polymorphic information content was 0.103–0.695. Shannon’s information index (I), observed heterozygosity (Ho) and expected heterozygosity (He) were 0.699, 0.188 and 0.436 respectively. The clustering results indicated that the castor…
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  • YB-1 downregulation attenuates UQCRC1 protein expression level in H9C2 cells and decreases the mitochondrial membrane potential
  • Abstract UQCRC1 is one of the 10 mitochondrial complex III subunits, this protein has a role in energy metabolism, myocardial protection, and neurological diseases. The upstream mechanism of the UQCRC1 protective effect on cardiomyocytes is currently unavailable. In order to explore the upstream molecules of UQCRC1 and elucidate the protective mechanism of UQCRC1 on cardiomyocytes in more detail, we focused on the nuclease-sensitive elementbinding protein 1 (YB-1). We hypothesized YB-1 acts as an upstream regulatory molecule of UQCRC1. This study found that YB-1 RNAi significantly reduces the expression of the UQCRC1 protein level (p < 0.05) and obviously decreases the mitochondrial…
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  • Effect of structure and function of paraoxonase-1 (PON-1) on organophosphate pesticides metabolism
  • Abstract Paraoxonase-1 (PON1) is an important enzyme in various pathologies such as pesticide poisoning, diabetes, atherosclerosis, neuronal disorders, and cancer, due to its multifunctional activity since it acts on different metabolites. However, one of its main functions is the hydrolysis of organophosphate (OP) compounds from pesticides that cause fatal poisoning at the level of the central nervous system (CNS). The objective of this review was to investigate whether the structure, genetics, and function of PON1 affect the metabolism of organophosphate pesticides or other abnormalities. Information was selected from articles in the database PubMed–NCBI (https://www.ncbi.nlm.nih.gov/ pubmed/) with a publication date between 2011…
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  • Piceatannol attenuates streptozotocin-induced type 1 diabetes in mice
  • Abstract As a natural analog of resveratrol, piceatannol (Pic) exhibits good antioxidant and anti-inflammatory activities in different disease models. However, the role of Pic in type 1 diabetes mouse model has not been reported yet. In this study, we investigated the in vivo effect of Pic in streptozotocin (STZ)-induced type 1 diabetic mice. Mice were injected with STZ to establish the type 1 diabetes mellitus (T1DM) model. After stable hyperglycemia was achieved, mice were then orally treated with Pic (40 mg/kg b.w., i.g.) for 30 days. The results indicated that Pic supplementation efficiently alleviated the typical symptoms associated with T1DM, including…
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  • The role of HBD-2, HBD-3, and calprotectin in the relationship between chronic periodontitis and atherosclerosis
  • Abstract This study was carried out to compare individuals diagnosed with atherosclerosis and periodontal periodontitis based on the degree of change in the human beta-defensins (HBD) HBD-2, HBD-3, and calprotectin. Atherosclerosis is the most frequently observed cardiovascular disease. Dental and periodontal infections are known to provide a considerable basis for atheroma plaque formation. The study group consists of a total number of 40 subjects, with 20 patients diagnosed with atherosclerosis and chronic periodontitis and 20 systemically healthy patients diagnosed with chronic periodontitis. Clinical periodontal and blood parameters and HBD-2, HBD-3, and calprotectin biomarkers in the gingival crevicular fluid were measured. In…
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  • Human umbilical cord blood mesenchymal stem cells conditioned media inhibits hypoxia-induced apoptosis in H9c2 cells by activation of the survival protein Akt
  • Abstract This work aimed to study the beneficial role of human umbilical cord blood-derived mesenchymal stem cellconditioned medium (MSC-CM) in hypoxia-induced apoptosis in H9c2 cardiomyoblasts, in which the serine/heroine kinases (Akt) pathway would be involved. For this, CM was collected by culturing MSCs in serum-free DMEM medium for 24 h, and paracrine factors were analyzed by protein chip. H9c2 cells were divided into the following groups: control group, hypoxia group, MSC-CM intervention group (CM group), MSC-CM + Akt phosphorylation inhibitor (LY294002) group (LY group). Apoptosis of the H9c2 cells was tested with chromatin dye Hoechst 33342 and FITC-conjugated Annexin V apoptosis…
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  • Effect of peroxiredoxin 6 on total and progressive motility of human spermatozoa after cryopreservation
  • Abstract Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation. However, freezing and thawing significantly reduces the total and progressive motility of human spermatozoa. In the present study, we explored the effects of peroxiredoxin 6 (PRDX6) on total and progressive motility of human spermatozoa after cryopreservation. Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6 (0 mM, 10−5 mM, 10−7 mM, and 10−9 mM, respectively). Postthaw total and progressive motility of sperms were measured. The results showed that in comparison with 0 mM, the concentrations of 10−5
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  • Decreased serum HMGB1 associated with M2 macrophage polarization and patients with calcific aortic valve disease
  • Abstract Except for the standard aortic valve replacement, no effective medical treatment is available to prevent or delay calcific aortic valve disease (CAVD) progression. Recently, macrophages and high-mobility group box 1 (HMGB1) are the most intriguing candidates in various inflammatory disorders. However, the association between serum HMGB1, CAVD, and macrophage polarization remains unclear. Therefore, we examined whether the level of serum HMGB1 is clinically associated with aortic valve calcification and whether HMGB1 treatment can promote macrophage differentiation toward M1 or M2 phenotype. This experimental study included 19 CAVD patients and 20 healthy controls whose serum HMGB1 levels were examined by ELISA…
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