Lu Guo^*, Duankai Chen^†, Xing Yin^‡, Qingfeng Shu^†

Oncology Research, Vol.27, No.4, pp. 487-494, 2019, DOI:10.3727/096504018X15323394008784

Abstract GSK-3 is a versatile protein kinase participating in many reactions. Currently, there is insufficient understanding of its influence on breast cancer (BC). In order to explore its influence on migration and invasion in BC, we investigated its expression in BC cell lines using qRT-PCR and Western blot (WB). Immunohistochemistry (IHC) was used to examine the potential of GSK-3 to predict clinical outcome in BC patients. GSK-3 knockdown was achieved using an shRNA plasmid vector in T47D cells. Our research explored the biological reactions and downstream pathways involved. We found excessive GSK-3 expression in BC tissues, which was correlated with worse… More >

Jia Liu^*, Ying Zhang^†, Shulan Sun^‡, Guirong Zhang^‡, Ke Jiang,§ Peixin Sun^*, Ye Zhang^*, Bing Yao^*, Rui Sui^*, Yi Chen^*, Xu Guo^*, Tao Tang^*, Ji Shi^*, Haiyang Liang^*, Haozhe Piao^*

Oncology Research, Vol.27, No.4, pp. 475-486, 2019, DOI:10.3727/096504018X15270916676926

Abstract Glioma is the most common malignant tumor of the central nervous system, and it is characterized by high relapse and fatality rates and poor prognosis. Bufalin is one of the main ingredients of Chan-su, a traditional Chinese medicine (TCM) extracted from toad venom. Previous studies revealed that bufalin exerted inhibitory effects on a variety of tumor cells. To demonstrate the inhibitory effect of bufalin on glioma cells and glioma stem-like cells (GSCs) and discuss the underlying mechanism, the proliferation of glioma cells was detected by MTT and colony formation assays following treatment with bufalin. In addition, we investigated whether bufalin… More >

Takashi Saito^*†, Atsuo Okamura^‡, Junichiro Inoue^†, Daisuke Makiura^†, Hisayo Doi^§, Kimikazu Yakushijin^¶, Hiroshi Matsuoka^¶, Yoshitada Sakai^†#, Rei Ono^*

Oncology Research, Vol.27, No.4, pp. 469-474, 2019, DOI:10.3727/096504018X15267574931782

Abstract Chemotherapy-induced peripheral neuropathy (CIPN) frequently occurs in lymphoma patients receiving R-CHOP, a drug combination therapy. Although severe CIPN may lead to reduction and/or discontinuation of the medication, predictive factors of CIPN have not been investigated sufficiently to date. We performed a retrospective exploratory research to determine associations between prevalence of severe CIPN and sociodemographic data, health characteristics, and medical conditions such as anemia at initial diagnosis. Forty patients (indolent lymphoma, n=9; diffuse large B-cell lymphoma; n=31) received R-CHOP therapy from September 2009 to July 2014. The median age of patients was 58 years (range=27–76 years). Statistical analyses were applied to… More >

Liang Wu, Yuefeng Li, Jingye Li, Deliang Ma

Oncology Research, Vol.27, No.4, pp. 459-467, 2019, DOI:10.3727/096504018X15193500663936

Abstract A large number of microRNAs (miRNAs) have been previously demonstrated to be dysregulated in breast cancer (BC), and alterations in miRNA expression may affect the initiation and progression of BC. This study showed that miR-664 expression was obviously reduced in BC tissues and cell lines. Resumption of the expression of miR-664 attenuated the proliferation and invasion of BC cells. The molecular mechanisms underlying the inhibitory effects of BC cell proliferation and invasion by miR-664 were also studied. Insulin receptor substrate 1 (IRS1) was identified as a novel and direct target of miR-664. In addition, siRNAmediated silencing of IRS1 expression mimicked… More >

Guoyun Li, Wei Zhang, Li Gong, Xiaoping Huang

Oncology Research, Vol.27, No.4, pp. 449-458, 2019, DOI:10.3727/096504017X15016337254623

Abstract MicroRNAs, a class of endogenous noncoding RNAs, regulate gene expression at the posttranscriptional level and thus take part in multiple biological processes. An increasing number of miRNAs have been found to be dysregulated in hepatocellular carcinoma (HCC) and are involved in liver tumorigenesis. In this study, miR- 125a-5p was found to be obviously downregulated much more in hepatitis B virus (HBV)-related HCC. To investigate the effects of miR-125a-5p, miR-125a-5p was overexpressed in HepG2.2.15 and HepG3X cells. The findings have indicated that overexpression of miR-125a-5p dramatically inhibited cell proliferation and induced cell apoptosis. Furthermore, overexpression of miR-125a-5p could significantly decrease the… More >

Gaowu Hu, Wei Pen, Ming Wang

Oncology Research, Vol.27, No.4, pp. 439-447, 2019, DOI:10.3727/096504018X15214994641786

Abstract Tripartite motif-containing 14 (TRIM14) is abnormally expressed in several human cancers. However, the function and expression of TRIM14 in human breast cancer are still largely unknown. To understand the biological function of TRIM14 in breast cancer, we measured the expression level of TRIM14. Cell proliferation and cell apoptosis were measured after TRIM14 overexpression or knockdown. Upregulation of TRIM14 was found in human breast cancer specimens and cell lines. Reduction of TRIM14 inhibited cell proliferation but increased cell apoptosis in the BT474 and MDA-MB-231 cell lines. Further study showed that knockdown of TRIM14 upregulated the expression of BAX while downregulating the… More >

Xin Chen^*, Mingzhe Li^†, Hongwei Zhou^*, Li Zhang^*

Oncology Research, Vol.27, No.4, pp. 431-437, 2019, DOI:10.3727/096504018X15201058168730

Abstract MicroRNA-132 (miR-132) has been demonstrated to be a tumor suppressor in several types of tumors. However, the expression and the role of miR-132 in human thyroid cancer are still poorly understood. The aim of the present study was to examine the potential roles and molecular mechanism of miR-132 in thyroid cancer. We found that miR-132 expression levels were significantly downregulated in thyroid cancer tissues and cell lines. Function assays showed that overexpression of miR-132 in TPC1 cells inhibited cell proliferation, migration, and invasion. Forkhead box protein A1 (FOXA1) was identified as a direct target of miR-132 in thyroid cancer cells.… More >

Hai-Tao Li, Dao-Yong Dong, Qiang Liu, Yi-Qin Xu, Langbo Chen

Oncology Research, Vol.27, No.4, pp. 423-429, 2019, DOI:10.3727/096504017X15030178624579

Abstract LACTB, a mitochondrial protein, was ubiquitously expressed in different mammalian tissues, such as liver, heart, and skeletal muscle. It has been shown that LACTB is downexpressed in breast cancers, and it suppresses the proliferation and promotes the apoptosis of breast cancers. However, its role in the progression and prognosis of glioma remains unknown. In this study, we analyzed the clinicopathological features and outcomes of LACTB expression in 98 glioma patients and investigated the effects of LACTB overexpression on the proliferation, invasion, and angiogenesis of glioma cells in vitro. We observed a significant decrease in LACTB expression in glioma, and downexpression… More >

Xuejian Liu^*1, Xia Wu^*1, Yanming Wang^†, Yuhua Li^*, Xiangli Chen^*, Wenchuan Yang^*, Lihua Jiang^*

Oncology Research, Vol.27, No.4, pp. 415-422, 2019, DOI:10.3727/096504018X15155538502359

Abstract Cluster of differentiation 47 (CD47) overexpression is common in various malignancies. This study investigated whether CD47 promotes human glioblastoma invasion and, if so, the underlying mechanisms involved. CD47 expression was found to be stronger in tissues of patients with glioblastoma and in various cancer cell lines than in normal controls. CD47 downregulation via siRNA suppressed invasion in vitro, whereas CD47 overexpression through plasmid transfection exerted the opposite effect. However, overexpression or knocking down of CD47 had no effect on cell proliferation. Moreover, CD47 expression was related to Akt phosphorylation at the cellular molecular level. Suppression of Akt with a specific… More >

Jihong Sun^*†1, Jingjing Li^†1, Zhixian Guo^†, Lu Sun^†‡, Chenghui Juan^§, Yubing Zhou^¶, Hongli Gu^†, Yan Yu^†, Qiuyue Hu^†, Quancheng’ Kan^¶, Zujiang Yu^†

Oncology Research, Vol.27, No.4, pp. 407-417, 2019, DOI:10.3727/096504018X15180451872087

Abstract Most cancers rely disproportionately on glycolysis for energy even in the presence of an adequate oxygen supply, a condition known as “aerobic glycolysis,” or the “Warburg effect.” Pyruvate dehydrogenase E1 subunit (PDHA1) is one of the main factors for the metabolic switch from oxidative phosphorylation (OXPHOS) to aerobic glycolysis and has been suggested to be closely associated with tumorigenesis. Here we observed that the PDHA1 protein was reduced in hepatocellular carcinoma (HCC) specimens by immunohistochemistry and Western blot, which was significantly associated with poor overall survival. To further analyze the function of PDHA1 in cancer cells, PDHA1 was upregulated in… More >