NING AN^1,#, HEQING PENG^2,#, MIN HOU³, DUOFENG SU², LIU WANG⁴, XIAOGANG SHEN^5,*, MING ZHANG^1,*

Oncology Research, Vol.31, No.3, pp. 307-316, 2023, DOI:10.32604/or.2023.028564

Abstract Zinc-finger proteins play different roles in cancer; however, the function of zinc-finger protein ZNF575 in cancer remains unclear. In the present study, we aimed to determine the function and expression of ZNF575 in colorectal cancer. Proliferation assay, colony formation assay, and tumor model in mice were used to investigate the function of ZNF575 after ectopic expression of ZNF575 in colorectal cancer (CRC) cells. RNA sequencing, ChIP, and luciferase assays were used to investigate the mechanism behind ZNF575 regulation of CRC cell growth. The expression of ZNF575 was determined by IHC staining in 150 pairs of malignant CRC tissues, followed by… More > Graphic Abstract

Muhammad Jan^1,*, Muhammad Anwar-Ul-Haq², Talha Javed³, Sadam Hussain^4,*, Ilyas Ahmad⁵, Muhammad Ashraf Sumrah⁶, Javed Iqbal⁷, Babar Hussain Babar⁸, Aqsa Hafeez⁹, Muhammad Aslam⁵, Muhammad Tahir Akbar¹⁰, Marjan Aziz⁶, Khadiga Alharbi¹¹, Izhar Ullah¹²

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1361-1375, 2023, DOI:10.32604/phyton.2023.026620

Abstract Abiotic stresses are among the major limiting factors for plant growth and crop productivity. Among these, salinity is one of the major risk factors for plant growth and development in arid to semi-arid regions. Cultivation of salt tolerant crop genotypes is one of the imperative approaches to meet the food demand for increasing population. The current experiment was carried out to access the performance of different rice genotypes under salinity stress and Zinc (Zn) sources. Four rice genotypes were grown in a pot experiment and were exposed to salinity stress (7 dS m⁻¹), and Zn (15 mg kg⁻¹ soil) was… More >

WEI SHI^1,2,3,#, JIANXIA LIN^1,2,3,#, RONG JIN^1,2,3, XIANJING XIE^1,2,3, YAN LIANG^1,2,3,*

BIOCELL, Vol.47, No.4, pp. 869-877, 2023, DOI:10.32604/biocell.2023.026037

Abstract Background: LncRNA DLX6-AS1 has been uncovered to exert effects on various cancers. Nevertheless, the impacts of DLX6-AS1 on endometrial cancer (EC) development remained obscure. The study explored the influence of DLX6-AS1 on EC progression via the microRNA (miR)-374a-3p/zinc-finger protein (ZFX) axis.Methods: EC cell lines were collected and DLX6-AS1, miR-374a-3p, and ZFX levels in EC cell lines were detected. The EC cells were transfected with DLX6-AS1, miR-374a-3p, and ZFX constructs to examine the biological functions of EC cells. The xenograft model was established for detecting tumor growth. Rescue experiments were conducted to verify the interaction of DLX6-AS1, miR-374a-3p, and ZFX in… More > Graphic Abstract

Xue Huang^1,*, Zeyu Chen¹, Ruizhao Yang¹, Guangzhu Feng²

Journal of Renewable Materials, Vol.11, No.5, pp. 2301-2319, 2023, DOI:10.32604/jrm.2023.025888

Abstract 1,3-Dioleoyl-2-palmitoylglycerol (OPO) has been a hotspot of functional oils research in recent years, but due to the high cost of sn-1,3 specific lipase in enzymatic synthesis and the lack of biocatalyst stability, large-scale industrial application is difficult. In this study, the prepared magnetic ZnFe₂O₄ was functionalized with dopamine to obtain ZnFe₂O₄@PDA, and the nano-biocatalyst ZnFe₂O₄@PDA@RML was prepared by immobilizing sn-1,3 specific lipase of Rhizomucor miehei lipase (RML) via a cross-linking method. The existence of RML on ZnFe₂O₄@PDA was confirmed by XRD, FTIR, SEM, and TEM. This strategy proved to be simple and effective because the lipase immobilized on magnetic nanoparticles… More > Graphic Abstract

Muhammad Zafar¹, Siraj Ahmed¹, Muhammad Kashif Munir¹, Nawal Zafar¹, Muhammad Saqib¹, Muhammad Aleem Sarwar², Saba Iqbal¹, Baber Ali³, Naveed Akhtar¹, Basharat Ali^4,*, Sadam Hussain^5,*, Muhammad Saeed⁶, Mohammad Khalid Al-Sadoon⁷, Aneela Gulnaz⁸

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 983-999, 2023, DOI:10.32604/phyton.2023.025813

Abstract Deficiencies of essential vitamins, iron (Fe), and zinc (Zn) affect over one-half of the world’s population. A significant progress has been made to control micronutrient deficiencies through supplementation, but new approaches are needed, especially to reach the rural poor. Agronomic biofortification of pulses with Zn, Fe, and boron (B) offers a pragmatic solution to combat hidden hunger instead of food fortification and supplementation. Moreover, it also has positive effects on crop production as well. Therefore, we conducted three separate field experiments for two consecutive years to evaluate the impact of soil and foliar application of the aforementioned nutrients on the… More >

Hengjiao Gao¹, Yuqing Xiong^1,*, Kaifeng Zhang¹, Shengzhu Cao¹, Mingtai Hu¹, Yi Li¹, Ping Zhang², Xiaoli Liu³

Journal of Renewable Materials, Vol.11, No.4, pp. 1715-1729, 2023, DOI:10.32604/jrm.2022.024023

Abstract The interface properties of Fe(101)/zinc silicate modified by organo-siloxane (KH-570) was studied by using the method of molecular dynamics simulation. By calculating the temperature and energy fluctuation of equilibrium state, equilibrium concentration distribution, MSD of layer and different groups, and interaction energy of two interface models, the influencing mechanism on the interface properties of adding organosiloxane into coating system was studied at the atomic scale. It shows that the temperature and energy of interface oscillated in a small range and it was exited in a state of dynamic equilibrium within the initial simulation stage (t < 20 ps). It can… More > Graphic Abstract

SEHRISH ABBAS¹, BUSHRA UZAIR^1,*, MAISRA AZHAR BUTT¹, FARID MENAA², BARKAT A. KHAN^3,*

BIOCELL, Vol.47, No.2, pp. 245-260, 2023, DOI:10.32604/biocell.2023.025311

Abstract The association of gut microflora and human health is being increasingly recognized, and the impact of gut microflora on the host is well characterized, including the body’s energy metabolism and immune system maintenance. Several human diseases, including metabolic, autoimmune, obesity, hypothyroidism, and intestinal disorders, are closely associated with gut dysbiosis. Inorganic nanoparticles (NPs) are extensively utilized in numerous fields due to their distinctive, attractive physicochemical properties. Estimation of the potential impacts of NPs, with a high number of microorganisms inside the human body (microbiota) and its genomes (microbiome), represents one of the most important aspects of nano-toxicology. This review article… More > Graphic Abstract

Sushuang Liu^1,2,#, Yanmin Liu^1,2,#, Chundong Liu¹, Xingwang Yu^2,3, Hao Ma^2,4,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 577-590, 2023, DOI:10.32604/phyton.2022.023738

Abstract Drought is a major abiotic stress limiting agricultural crops production worldwide. In our study, we isolated a novel C2H2-type zinc finger protein gene ZF2 from chickpea. ZF2 consisted of 232 amino acids with two QALGGH motifs in Cys2/His2 zinc finger domain. Transient expression analysis of ZF2:GFP fusion protein showed that ZF2 was a nuclear localized protein. In the yeast assay system, the full-length of ZF2 did not show transcriptional activation. Expression of ZF2 gene was enhanced by treatments of several abiotic stresses and phytohormones. The promoter region of ZF2 contained multiple stress- and hormone-related cis-elements. Overexpression of ZF2 in Arabidopsis… More >

Bo Xia^*, Lei Wang^†, Li Feng^*, Baofang Tian^*, Yuanjie Tan^‡, Baoyin Du^*

Oncology Research, Vol.27, No.1, pp. 89-98, 2019, DOI:10.3727/096504018X15199511344806

Abstract Osteosarcoma is the most common primary malignant bone tumor in children and adolescents. This study aimed to explore the effects of long noncoding RNA CAT104 and microRNA-381 (miR-381) on osteosarcoma cell proliferation, migration, invasion, and apoptosis, as well as the underlying potential mechanism. We found that CAT104 was highly expressed in osteosarcoma MG63 and OS-732 cells. Knockdown of CAT104 significantly inhibited OS-732 cell proliferation, migration, and invasion, but promoted cell apoptosis. CAT104 regulated the expression of miR-381, and miR-381 participated in the effects of CAT104 on OS-732 cells. Zinc finger E-box-binding homeobox 1 (ZEB1) was a direct target gene of… More >

Cong Ye^*, Yubo Hu^†, Junrong Wang^*

Oncology Research, Vol.27, No.2, pp. 183-192, 2019, DOI:10.3727/096504018X15201124340860

Abstract A large number of microRNAs (miRNAs) are aberrantly expressed in cervical cancer and play crucial roles in the onset and progression of cervical cancer by acting as either an oncogene or a tumor suppressor. Therefore, investigation of the expression, biological roles, and underlying mechanisms of miRNAs in cervical cancer might provide valuable therapeutic targets in the treatment for patients with this disease. In this study, miRNA- 377 (miR-377) was downregulated in cervical cancer tissues and cell lines. Decreased miR-377 expression was strongly correlated with the International Federation of Gynecology and Obstetrics (FIGO) stage, lymph node metastasis, and distant metastasis in… More >