Huiru Deng#, Huan Tian#, Liuyan Yang, Siyang Ou, Hui Wang, Guangdong Geng*, Suqin Zhang*
Phyton-International Journal of Experimental Botany, Vol.93, No.12, pp. 3273-3291, 2024, DOI:10.32604/phyton.2024.058638
- 31 December 2024
Abstract Hot pepper (Capsicum annuum var. conoides) is a significant vegetable that is widely cultivated around the world. Currently, global climate change has caused frequent severe weather events, and waterlogging stress harms the pepper industry by affecting the planting period, growth conditions, and disease susceptibility. The gene CaABI3/VP1-1 could improve pepper waterlogging tolerance. In order to explore the upstream regulatory mechanism of CaABI3/VP1-1, a high-quality standardized yeast hybrid library was successfully constructed for yeast one-, two-, and three-hybrid screening using pepper ‘ZHC2’ as the experimental material, with a library recombinant efficiency of up to 100%. The length of inserted… More >
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