Alfaro-Corres Arnoldo Enrique1,#, Lecona-Guzman Carlos Alberto1,#, Gutiérrez-Miceli Federico Antonio1, Martínez-Esteves Manuel2, Silverio-Gómez María del Carmen3, Medina-Lara María de Fátima2, Ruiz-Lau Nancy4,*
Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 629-643, 2023, DOI:10.32604/phyton.2022.023970
- 12 October 2022
Abstract The effect of water deficit was determined on both in vitro and soil seedling as well as in cells in suspension of Agave americana L. In order to do the establishment of cells, the formation of callus was induced; for it two auxins were evaluated: 2,4-dichlorophenoxyacetic acid (2,4-D) and 4-mino-3,5,6-trichloropicolinic acid (picloram) at three concentrations (0.25, 0.5 and 0.75 mg L−1) in three explants (leaf, root and meristems) cultured in MS semisolid medium. The callogenesis response was related to the type and section of the explant, as well as the regulator used, and a cell suspension was established… More >