Enyan Chen^1,#, Jingyu Jia^1,3,#, Jiaju Sun¹, Jie Wang², Xinxin Chen¹, Xin Li^1,3,4,*

Phyton-International Journal of Experimental Botany, Vol.93, No.11, pp. 2837-2856, 2024, DOI:10.32604/phyton.2024.057932 - 30 November 2024

Abstract During post-harvest storage of Cucumis sativus fruit, the application of trypsin treatment could increase flavonoid compound levels and reduce oxidative damage. To investigate the mechanism of trypsin-induced flavonoid biosynthesis in C. sativus, we conducted a combined analysis of transcriptomics and widely targeted metabolomics. One hundred and seventy-five significantly different metabolites were obtained from metabolomics data. The kyoto encyclopedia of genes and genomes (KEGG) functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis pathway. By combining the results of the weighted gene co-expression network analysis (WGCNA) with the 130 upregulated phenylpropanoid metabolites, 22… More >

Bairu Li¹, Jingyu Jia¹, Hemin Wang¹, Jiaju Sun¹, Enyan Chen¹, Xin Li^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.93, No.5, pp. 885-900, 2024, DOI:10.32604/phyton.2024.050450 - 28 May 2024

Abstract Trypsin, a novel superoxide scavenger, significantly enhances the storage quality of Hylocereus undatus (H. undatus). To elucidate the preservation mechanism of trypsin on H. undatus, a widely targeted metabolomic analysis, and transcriptomics analysis were conducted. Firstly, a total of 453 metabolites were identified, with organic acids and their derivatives constituting the largest proportion (25%). Amino acids and their metabolites, prominent among organic acids, were further analyzed. Among them, 73 metabolites were associated with amino acids, and 37 exhibited significant differences. The most enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway was arginine biosynthesis (map00220), with polyamine metabolites… More >

JIANHUA LI^1,#, YAOGANG ZHANG^1,2,#, TAO ZHANG³, MEIYUAN TIAN¹, JING HOU¹, DENGLIANG HUANG¹, YAN CHENG^1,3, ZHU MAN^1,3, XIAOMING SU^1,3, ZHIQIN LI¹, SIXIAN TONG¹, XUAN ZHANG^1,3, JUN DENG^1,3, YUN DONG^1,3, YANYAN MA^1,2,3,*

BIOCELL, Vol.44, No.2, pp. 209-215, 2020, DOI:10.32604/biocell.2020.08941 - 27 May 2020

Abstract The aim of this study was to find a way to efficiently separate neuronal cells from the cerebral cortex of adult rats, providing a reference method for rapid acquisition of neuronal cells from the adult rat brain. Fifteen SD rats were randomly divided into three groups, with five SD rats in each group. Then, neuron cells were isolated from the adult rat cerebral cortex by the grinding method, the trypsin method, and the collagenase II method, respectively. The expression of anti-NeuN in the neurons of each group was analyzed by flow cytometry. The acquisition rates More >

Sinagawa-García SR¹, A Gutiérrez-Díez¹, A Mora-Olivo², MC Juárez-Aragón², JA Torres-Castillo²

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 358-367, 2015, DOI:10.32604/phyton.2015.84.358

Abstract A general biochemical description of an aqueous extract obtained from Ibervillea sonorae root was reported; furthermore, a histological description for the root is included. A bromatological analysis was carried out, and it indicated that the study nutritional variables were similar to those reported in other edible tuberous roots including sweet potatoe, mandioca, bean yam and arracacha. For the first time, the presence of an active proteinase at alkaline pH and the inhibitory activity of trypsin were reported for this root. Several medical and bioactive properties have been attributed to the wereke root. Nevertheless, deeper studies are More >

Q Wang^*, YP Zheng^∗,†, HJ Niu^∗,‡

Molecular & Cellular Biomechanics, Vol.7, No.1, pp. 45-58, 2010, DOI:10.3970/mcb.2010.007.045

Abstract This study aims to obtain osmosis-induced swelling strains of normal and proteoglycan (PG) depleted articular cartilage using an ultrasound system and to investigate the changes in its mechanical properties due to the PG depletion using a layered triphasic model. The swelling strains of 20 cylindrical cartilage-bone samples collected from different bovine patellae were induced by decreasing the concentration of bath saline and monitored by the ultrasound system. The samples were subsequently digested by a trypsin solution for approximately 20 min to deplete proteoglycans, and the swelling behaviors of the digested samples were measured again. The… More >

KIVANÇ ERGEN^*, MUHAMMET BEKTAŞ^**, SINA GÖKÇE^**, RÜSTEM NURTEN^**

BIOCELL, Vol.31, No.1, pp. 61-66, 2007, DOI:10.32604/biocell.2007.31.061

Abstract Eukaryotic elongation factor 2 (eEF-2) can undergo ADP-ribosylation in the absence of diphtheria toxin. The binding of free ADP-ribose and endogenous transferase-dependent ADP-ribosylation were distinct reactions for eEF-2, as indicated by different findings. Incubation of eEF-2 tryptic fragment 32/33 kDa (32F) with NAD was ADP-ribosylated and gave rise to the covalent binding of ADP-ribose to eEF-2. 32F was revealed to be at the C-terminal by Edman degradation sequence analysis.
In our study, the elution of 32F from SDS-PAGE was ADP-ribosylated both in the presence and absence of diphtheria toxin. These results suggest that endogenous ADP-ribosylation More >