Dmytro A. Kiriziy¹, Oksana V. Dubrovna¹, Oksana G. Sokolovska-Sergiienko¹, Alina S. Holoboroda¹, Victor V. Rohach^1,2, Oleg O. Stasik^1,*

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.075371 - 30 January 2026

Abstract Water scarcity severely constrains the genetic potential of wheat yield worldwide. Proline is among the most versatile stress-related metabolites in plants, and targeting genes involved in proline synthesis and degradation represents a promising strategy for developing drought-tolerant wheat genotypes. This study evaluates the performance of the photosynthetic apparatus in transgenic wheat line with RNAi-mediated suppression of proline dehydrogenase (ProDH) and in the original (wild-type) genotype, under both drought and recovery conditions. Drought was induced at the flowering stage by lowering soil moisture to 30% field capacity for 7 days, compared with 70% field capacity in… More >

Shiyang Li^1,#, Xue-Ao Liu^2,#, Lizi Zhao², Huiqing Huang³, Bei Li², Zhizhong Song², Meixia Liang², Hongxia Zhang^2,3, Limin Wang^2,*, Shenglin Zhou^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 761-772, 2021, DOI:10.32604/phyton.2021.016314 - 30 March 2021

Abstract Seven in absentia (SINA) family proteins play a central role in plant growth, development and resistance to abiotic stress. However, their biological function in plant response to cold stress is still largely unknown. In this work, a seven in absentia gene IbSINA5 was isolated from sweet potato. Quantitative real-time polymerase chain reaction (qRT-PCR) analyses demonstrated that IbSINA5 was ubiquitously expressed in various tissues and organs of sweet potato, with a predominant expression in fibrous roots, and was remarkably induced by cold, drought and salt stresses. Subcellular localization assays revealed that IbSINA5-GFP fusion protein was mainly localized in cytoplasm… More >

BEEMNET MENGESHA KASSAHUN^1,#, BEUM-CHANG KANG^2,#, SU-JI BAE², YE JIN NAM¹, GRETEL FONSECA MUNDO¹, GA-HUI KANG¹, KYOUNGOOK KIM³, JEUNG-SUL HAN^1,4,*

BIOCELL, Vol.45, No.1, pp. 199-215, 2021, DOI:10.32604/biocell.2021.012353 - 26 January 2021

Abstract Solanum lycopersicum ‘Heinz 1706’ is a pioneer model cultivar for tomato research, whose whole genome sequence valuable for genomics studies is available. Nevertheless, a genetic transformation procedure for this cultivar has not yet been reported. Meanwhile, various genome editing technologies such as transfection of clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) ribonucleoprotein complexes into cells are in the limelight. Utilizing the Cas9-expressing genotype possessing a reference genome can simplify the verification of an off-target effect, resolve the economic cost of Cas9 endonuclease preparation, and avoid the complex assembly process together with single-guide RNA (sgRNA) in… More >