Xiaoqing Jiang^1,2,#, Pengchong Li^1,2,#, Hongchuang Liu^1,2, Wenjie Dong^1,2, Wenjing Liu^1,2, Di Wu^1,2, Jianping Xue^1,2, Fenglan Zhao^1,2,*, Yongbo Duan^1,2,*

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074434 - 30 January 2026

Abstract As its tuberous alkaloids induce valuable pharmacological effects, Pinellia ternata has considerable clinical value. However, its production currently fails to meet its demand. In vitro microtuber culture, combined with salicylic acid (SA) elicitation, may provide an effective alternative to traditional field production. In this study, an in vitro P. ternata microtuber induction system was developed and used to evaluate SA-induced elicitation of alkaloid accumulation. The quality of in vitro microtubers was assessed by total alkaloid measurement, a cytotoxicity assay, and transcriptomic analysis. With or without SA treatment, P. ternata microtuber induction was achieved within 60 d using petiole-derived explants, with… More >

Yun-Im Kang¹, Youn Jung Choi¹, Su Young Lee¹, Young-Ran Lee¹, Ki-Byung Lim^2,3, Yun-Jae Ahn^2,3,*

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.073138 - 30 January 2026

Abstract Cucumber mosaic virus (CMV) threatens lily production by reducing floral quality and enabling carry-over via infected planting stock. To explore tissue-specific host responses, we analyzed a legacy, single-replicate RNA-seq dataset from two cultivars, ‘Cancun’ and ‘Connecticut King’ (CK), profiling leaf (source) and bulb (sink) tissues at 0 and 28 days post-inoculation (dpi), alongside leaf DAS-ELISA. Principal component analysis indicated that tissue identity dominated the transcriptome (PC1 = 47.7%), with CMV treatment driving within-tissue shifts over time. Exploratory Gene Ontology/KEGG summaries and a focused marker panel revealed a consistent split: in leaves, genes linked to jasmonate/WRKY-associated… More >

Kai Gui^1,#, Tianyi Yang^1,#, Chengying Xiong¹, Yue Wang¹, Zhiqiang He¹, Wuxian Li^2,3,*, Min Tang^1,*

Oncology Research, Vol.34, No.1, 2026, DOI:10.32604/or.2025.070143 - 30 December 2025

Abstract Objectives: The mechanism by which specific tumor subsets in colorectal cancer (CRC) use alternative metabolic pathways, particularly those modulated by hypoxia and fructose, to alter the tumor microenvironment (TME) remains unclear. This study aimed to identify these malignant subpopulations and characterize their intercellular signaling networks and spatial organization through an integrative multi-omics approach. Methods: Leveraging bulk datasets, single-cell RNA sequencing, and integrative spatial transcriptomics, we developed a prognostic model based on hypoxia-and fructose metabolism-related genes (HFGs) to delineate tumor cell subpopulations and their intercellular signaling networks. Results: We identified a specific subset of stanniocalcin-2 positive (STC2+)… More > Graphic Abstract

Amber Hassan¹, Badr Hafiz², Taghreed Alsinani³, Rakan Bokhari⁴, Dahlia Mirdad⁵, Awab Tayyib⁵, Alaa Alkhotani⁶, Ahmad Fallata⁷, Iman Mirza⁸, Eyad Faizo^9,10, Saleh Baeesa², Huda Alghefari¹¹, Maher Kurdi^11,*

Oncology Research, Vol.33, No.11, pp. 3293-3325, 2025, DOI:10.32604/or.2025.070031 - 22 October 2025

Abstract Background: Glioblastoma (GBM) remains the most aggressive primary brain tumour in adults, marked by pronounced cellular heterogeneity, diffuse infiltration, and resistance to conventional treatment. In recent years, transcriptomic profiling has provided valuable insights into the molecular mechanisms that govern the progression of glioblastoma. This systematic review aims to synthesise the current literature on dysregulated gene expression in GBM, focusing on gene signatures associated with stemness, immune modulation, extracellular matrix remodelling, metabolic adaptation, and therapeutic resistance. Methods: We conducted a systematic search of PubMed, The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), and the GlioVis… More >

Jing Zhang^1,#, Huifen Qiao^1,#, Shenglong Wang^1,#, Jiawei Yuan¹, Qingsong Ba¹, Gensheng Zhang^1,2,*, Guiping Li^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2701-2718, 2025, DOI:10.32604/phyton.2025.070017 - 30 September 2025

Abstract Wheat stripe rust, a devastating disease caused by the fungal pathogen Puccinia striiformis f. sp. tritici (Pst), poses a significant threat to global wheat production. Growing resistant cultivars is a crucial strategy for wheat stripe rust management. However, the underlying molecular mechanisms of wheat resistance to Pst remain incompletely understood. To unravel these mechanisms, we employed high-throughput RNA sequencing (RNA-Seq) to analyze the transcriptome of the resistant wheat cultivar Mianmai 46 (MM46) at different time points (24, 48, and 96 h) post-inoculation with the Pst race CYR33. The analysis revealed that Pst infection significantly altered the expression of genes… More >

Hafiza Muniba Din Muhammad¹, Safina Naz¹, Zarina Bibi², Mohamed A. A. Ahmed³, Alina-Stefania Stanciu⁴, Riaz Ahmad^5,*

Phyton-International Journal of Experimental Botany, Vol.94, No.9, pp. 2631-2648, 2025, DOI:10.32604/phyton.2025.069474 - 30 September 2025

Abstract The production of crops is badly affected by climate change globally. Mitigation of adverse effects of climate change is in need of time through different management practices such as developing tolerant genetic resources, hormonal applications to boost defense systems, nanoparticles, and balanced fertilization. The nano-hormonal synergy had the potential to mitigate the adverse effects of climate change by modulation of morpho-physiological and biochemical activities. Plant growth, yield, and quality can be enhanced with the supplementation of nano-hormonal interactions. Therefore, the current study explores the synergy between nanoparticles and phytohormonal use. The nanoparticles, even in low… More >

Siyang Ou¹, Liuyan Yang¹, Tingting Yuan¹, Mutong Li¹, Guohui Liao², Wanping Zhang¹, Guangdong Geng^1,*, Suqin Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2441-2460, 2025, DOI:10.32604/phyton.2025.068368 - 29 August 2025

Abstract Chive (Allium ascalonicum L.), a seeding-vernalization-type vegetable, is prone to bolting. To explore the physiological and molecular mechanisms of its bolting, bolting-prone (‘BA’) and bolting-resistant (‘WA’) chives were sampled at the vegetative growth, floral bud differentiation, and bud emergence stages. No bolting was observed in bolting-resistant ‘WA’ on the 130th day after planting, whereas the bolting reached 39.22% in bolting-prone ‘BA’, which was significantly higher than that of ‘WA’. The contents of gibberellins, abscisic acid, and zeatin riboside after floral bud differentiation in ‘WA’ were significantly less than in ‘BA’, whereas the indoleacetic acid content in… More >

Lina Song¹, Caijie Yi¹, Shiwei Zhao¹, Yuxin Peng¹, Zijing Li¹, Yuqiang Zhang ², Hua Zhang¹, Helan Qin¹, Huali Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.8, pp. 2593-2613, 2025, DOI:10.32604/phyton.2025.068133 - 29 August 2025

Abstract Naturally occurring yellow leaf mutants are an important resource for studying pigment content and biosynthesis, as well as related gene expression. In our ongoing cultivation of Rehmannia chingii H. L. Li, we found an off-type yellow plant. The yellowing started with the new leaves and gradually spread downward until the entire plant exhibited a stable shade of yellow. We studied the differences in the chlorophyll and carotenoid content, carotenoid profile, and transcriptome of this yellow-leaf mutant (P2). Compared to the wild-type R. chingii plant (P1), P2 leaves had significantly lower chlorophyll and carotenoid content. LC-MS/MS analysis revealed More >

Yinghui Ye^1,#, Yulou Luo^2,#, Yutian Sun³, Yujie Zhang¹, Jiaxin Lin⁴, Ziling Yang⁵, Anping Xu^6,*, Bei Xue^1,*

Oncology Research, Vol.33, No.6, pp. 1383-1404, 2025, DOI:10.32604/or.2025.062584 - 29 May 2025

Abstract Objectives: The tumorigenic progression of Lung adenocarcinoma (LUAD), the predominant NSCLC subtype, is predominantly driven by co-occurring mutations in KRAS proto-oncogene (KRAS)/Tumor protein p53 (TP53). However, their impact on tumor microenvironment (TME) heterogeneity, particularly neutrophil dynamics, remains poorly understood. This present study aims to elucidate how KRAS/TP53 mutations reprogram the TME and develop a neutrophil-centric prognostic signature for LUAD. Methods: Leveraging single-cell RNA sequencing data and transcriptome data, neutrophil subpopulations were identified using Seurat and CellChat R packages, with trajectory analysis via Monocle2 R package. High-dimensional weighted gene co-expression network analysis (hdWGCNA), univariate Cox regression,… More >

Chieh-Jen Wu^1,#, Yu-He Li^2,#, Hsin-Hung Chen^3,*

BIOCELL, Vol.49, No.5, pp. 857-872, 2025, DOI:10.32604/biocell.2025.064071 - 27 May 2025

Abstract Background: Serum- and glucocorticoid-induced kinase 1 (SGK1) is a member of the serine/threonine kinase family, playing a crucial role in regulating ion channel function, hormone secretion, cellular growth, survival mechanisms, and neuronal activity. SGK1 is implicated in kidney diseases, hypertension, and metabolic syndromes, influencing salt intake, renal growth, and renal potassium (K⁺) excretion during mineralocorticoid overdose. Although SGK1’s renal functions have been explored, comprehensive identification of SGK1-related genes and signaling cascades remains limited. Objectives: This research sought to explore the cellular mechanisms and signaling pathways influenced by SGK1 in rat kidney cells. Methods: NRK-52E cells, derived… More >