PAOLA TALIA1 , EDUARDO J. GREIZERSTEIN1 , H. ESTEBAN HOPP1,2 , NORMA PANIEGO1 , LIDIA POGGIO2 AND RUTH A. HEINZ1,2*
BIOCELL, Vol.35, No.1, pp. 19-28, 2011, DOI:10.32604/biocell.2011.35.019
Abstract Bacterial artificial chromosome - fluorescence in situ hybridization (BAC-FISH) and cyclingprimed in situ labeling (C-PRINS) techniques were evaluated for integration of physical and genetic maps of
sunflower (Helianthus annuus L.). Single-site SSR markers were selected from three linkage groups of a
high-density sunflower genetic map. This selection was based on previously identified QTL associated to S.
sclerotiorum. These markers were used to select BACs contaning single copy sequences for BAC-FISH
aplication. Blocking of highly dispersed repetitive sunflower sequences reduced unspecific hybridization,
and allowed the detection of specific signals for BACs containing SSR markers HA4222 and HA2600, anchored to LG More >
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