Ajith Rajapaksha1,2, Michael Fink1, Brian A. Todd1
Molecular & Cellular Biomechanics, Vol.12, No.3, pp. 215-230, 2015, DOI:10.3970/mcb.2015.012.215
Abstract Delivery of therapeutic agents to the eye requires efficient transport through cellular and extracellular barriers. We evaluated the rate of diffusive transport in excised porcine corneal stroma using fluorescently labeled dextran molecules with hydrodynamic radii ranging from 1.3 to 34 nm. Fluorescence correlation spectroscopy (FCS) was used to measure diffusion coefficients of dextran molecules in the excised porcine corneal stroma. The preferential sensitivity of FCS to diffusion along two dimensions was used to differentially probe diffusion along the directions parallel to and perpendicular to the collagen lamellae of the corneal stroma. In order to develop… More >
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