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  • Open Access

    ARTICLE

    Micropropagation of Daylily (Hemerocallis fulva) from Crown-Tip Explants and Assessment of Somaclonal Variation of in Vitro-Propagated Plants Using SCoT Markers

    Esraa E. Shalan1, Said S. Soliman1, Ahmed A. Mahmoud1, Jameel M. Al-Khayri2,*, Salha M. ALshamrani3, Fatmah A. Safhi4, Areej S. Jalal4, Diaa Abd El-Moneim5, Abdallah A. Hassanin1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2183-2196, 2023, DOI:10.32604/phyton.2023.028537 - 29 May 2023

    Abstract Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication. In this research, nine multiplication media (MM) augmented with different concentrations of 6-benzyl adenine (BA), Kinetin (Kin), and Thidiazuron (TDZ), Three rooting media (RM) supplemented with three levels of α-naphthalene acetic acid (NAA) and three types of soil mixtures (v/v); Coco peat/Vermiculite/Sand (CVS), Peat moss/Perlite/Sand (PPS) and Peat moss/Perlite (PP) were used in the micropropagation protocol of daylily plants. MM2 showed the maximum shoot length and the number of leaves, while MM9… More >

  • Open Access

    ARTICLE

    Vital Parameters Assessments of Starvation Tolerance of in vitro Populus alba Culture

    Mohamed Fathy Ahmed1, Eman Zakaria Ahmed2, Salman Aloufi3, Mohammed Alqurashi3, Amal Alyamani3, Eman Fayad3, Eman Tawfik Hussien2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 889-902, 2022, DOI:10.32604/phyton.2022.018600 - 09 December 2021

    Abstract Populus alba is a large woody deciduous plant. The plant has been introduced to shooting, then multiplication of rooting on Murashige and Skoog (MS) medium. This work was designed to estimate the effect of two factors (low levels of 1-Naphthaleneacetic acid NAA and sucrose) on P. alba response resulting in 6 treatments compared to the control, with twelve measured responses. There was a significant difference in some measurements in morphology, like plantlets fresh-weight, shoot-, root-length, and leaf number. In the physiological measurements, there were significant differences in all the measured parameters. The low concentrations of sucrose and… More >

  • Open Access

    ARTICLE

    Stability of protein patterns in coffee seedlings regenerated by somatic embryogenesis

    Menéndez-Yuffá1 A, L Ríos-Bolívar2

    Phyton-International Journal of Experimental Botany, Vol.77, pp. 49-64, 2008, DOI:10.32604/phyton.2008.77.049

    Abstract Regeneration and multiplication of coffee (Coffea arabica L.) through somatic embryogenesis can be efficiently achieved through different methods. However, it is very important to analyze the progenies obtained by these methods in order to establish their genetic stability. The objectives of this research were (1) to determine the protein electrophoretic patterns in leaves of coffee vitroplants which were regenerated through somatic embryogenesis, and (2) to compare these patterns with those of the explant donor plants. The protein patterns of some coffee genotypes were initially determined to detect possible differences among them. Each analyzed genotype showed a More >

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