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  • Open Access

    ARTICLE

    Linkage Mapping and QTL Analysis of Isoflavones Composition in Soybean Seeds

    Songnan Yang1, Miao Zhang1, Rongrong Yao1, Liangyu Chen1, Weixuan Cong1, Dan Yao2, Jian Zhang1,3, Jun Zhang1,*, Xueying Li1,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2209-2225, 2024, DOI:10.32604/phyton.2024.055046 - 30 September 2024

    Abstract The high isoflavones content of soybeans is an important breeding goal due to the demonstrated benefits of isoflavones to human health and their association with plant resistance. In this study, quantitative trait loci (QTL) mapping for soybean isoflavone aglycones, including daidzin, glycerin, and genistin, and total isoflavones content was performed in a population of 178 F2:6 recombinant inbred lines (RILs) which was generated from a cross between varieties Jinong 17 and Jinong 18. A genetic linkage map covering 1248 cM was constructed using the simple sequence repeat (SSR) molecular markers. The results revealed 22 isoflavone-related QTLs,… More >

  • Open Access

    ARTICLE

    The whole-genome survey of Acer griseum, its polymorphic simple sequence repeats development and application

    XIAO-JUN ZHOU1,*, YU-WEI TIAN1, RUI-HAN LI2

    BIOCELL, Vol.47, No.8, pp. 1907-1913, 2023, DOI:10.32604/biocell.2023.030191 - 28 August 2023

    Abstract Background: Acer griseum Pax is an endangered species endemic to China with both ornamental and economic value. However, the lack of information on its genome size and characteristics hinders further work at the genome level. Methods: This paper applied bioinformatics methods to predict the characteristics and patterns of the A. griseum genome, providing an important basis for formulating its whole-genome sequencing scheme. This study also characterized the simple sequence repeats (SSRs) of A. griseum, laying the foundation for the development and application of genome-wide SSR markers. In this study, PE150 sequencing was performed by the BGI MGISEQ platform,… More >

  • Open Access

    ARTICLE

    Quantitative Trait Loci (QTL) Mapping and Marker Analysis of Fatty Acids in Peanut

    Xiao Han, Songnan Yang, Xueying Li, Qiulin Wu, Yongyi Xing, Jun Zhang*, Fenglou Ling*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2577-2589, 2023, DOI:10.32604/phyton.2023.029440 - 28 July 2023

    Abstract Peanut, with high oil content, has been a major oil and food crop globally. The compositions of the fatty acids are the common factors in determining the oil quality. In the present study, an F2 segregated population with 140 individuals derived from the cross of Weihua8 (a cultivar) and 12L49 (a line with high oleic acid concentration) was used to construct a genetic map and conduct QTL mapping analysis. A total of 103 polymorphic SSR primers were utilized for genotyping the RILs and finally generating the SSR loci. Within the 103 SSR loci, a genetic… More >

  • Open Access

    ARTICLE

    Molecular characterization of sweet potato (Ipomoea batatas [L.] Lam) germplasms for desirable traits by using simple sequence repeats markers

    KHANDAKAR ABU MD MOSTAFIZAR RAHMAN1,2, ABDUL SHUKOR JURAIMI2,*, MD. REZWAN MOLLA3, MUHAMMAD ASYRAF MD HATTA4, ZULKEFLY BIN SULAIMAN5, SHAMIMA SULTANA6, AHMED GABER7, BENUKAR BISWAS8, AKBAR HOSSAIN9,*

    BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135 - 26 September 2022

    Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information… More >

  • Open Access

    ARTICLE

    Development and characterization of Simple Sequence Repeat (SSR) markers from the genomic sequence of sweet potato [Ipomoea batatas L. (Lam)]

    HANNA AMOANIMAA-DEDE, JIACHENG ZHANG, CHUNTAO SU, HONGBO ZHU*

    BIOCELL, Vol.45, No.4, pp. 1095-1105, 2021, DOI:10.32604/biocell.2021.015053 - 22 April 2021

    Abstract Sweet potato is a multifunctional root crop with many essential nutrients and bioactive compounds. Due to its genetic complexity and lack of genomic resources, efficient genetic studies and cultivar development lag far behind other major crops. Simple sequence repeats (SSRs) offer an effective molecular marker technology for molecular-based breeding and for locating important loci in crop plants, but only a few have previously been developed in sweet potato. To further explore new SSR markers and accelerate their use in sweet potato genetic studies, genome-wide characterization and development of SSR markers were performed using the recently… More >

  • Open Access

    ARTICLE

    Characterization of full-length transcriptome and mechanisms of sugar accumulation in Annona squamosa fruit

    REN FANG#, WEIXIONG HUANG#, JINYAN YAO, XING LONG, JI ZHANG, SHUANGYUN ZHOU, BIAO DENG, WENZHONG TANG, ZHENYU AN*

    BIOCELL, Vol.44, No.4, pp. 737-750, 2020, DOI:10.32604/biocell.2020.012933 - 24 December 2020

    Abstract Annona squamosa is a multipurpose fruit tree employed in nutritional, medicinal, and industrial fields. Its fruit is significantly enriched in sugars, making it an excellent species to study sugar accumulation in fruit. However, the scarcity of genomic resources hinders genetic studies in this species. This study aimed at generating large-scale genomic resources in A. squamosa and deciphering the molecular basis of its high sugar content. Herein, we sequenced and characterized the full-length transcriptome of A. squamosa fruit using PacBio Iso-seq. In addition, we analyzed the changes in sugar content over five fruit growth and ripening stages, and we… More >

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