Xuexin Gao^*, Xuezhen Gao^†, Chao Li^*, Yukun Zhang^*, Lei Gao^‡

Oncology Research, Vol.24, No.5, pp. 337-343, 2016, DOI:10.3727/096504016X14666990347671

Abstract Lung cancer remains a critical health concern worldwide. Long noncoding RNAs with ultraconserved elements have recently been implicated in human tumorigenesis. The present study investigated the role of ultraconserved element 338 (uc.338) in the regulation of cell proliferation and metastasis in human lung cancer. Our data showed that the expression of uc.338 in lung cancer was remarkably increased in vivo and in vitro. Depletion of uc.338 with specific siRNA interference retarded the cell proliferative rate in lung cancer cell lines NCI-H929 and H1688. Furthermore, knockdown of uc.338 caused cell cycle arrest in the G₀/G₁ phase in More >

Hai Liu^*, Xuanxuan Wang^*, Aihua Huang^†, Huaping Gao^‡, Yikan Sun^§, Tingting Jiang^*, Liming Shi^*, Xianjie Wu^¶, Qinghua Dong^#, Xiaonan Sun^*

Oncology Research, Vol.27, No.1, pp. 29-38, 2019, DOI:10.3727/096504018X15179694020751

Abstract Artemis is a key protein of NHEJ (nonhomologous end joining), which is the major pathway for the repair of IR-induced DSBs in mammalian cells. However, the expression of Artemis in tumors and the influence of silencing Artemis on tumor sensitivity to radiation have not been investigated fully. In this study, we investigated how the expression levels of Artemis may affect the treatment outcome of radiotherapy and chemotherapy in colorectal cancer cells. First, we found that the expression of Artemis is strong in some human rectal cancer samples, being higher than in adjacent normal tissues using… More >

SUSHMITHA SRIRAMULU¹, SARUBALA MALAYAPERUMAL¹, SUMAN K. NANDY², ANTARA BANERJEE¹, MUSTHAFA MOHAMED ESSA^3,4, SARAVANABABU CHIDAMBARAM⁵, M. WALID QORONFLEH^6,7, SURAJIT PATHAK^1,*

BIOCELL, Vol.45, No.3, pp. 563-576, 2021, DOI:10.32604/biocell.2021.014756

Abstract Colon cancer is the third major cause of cancer deaths, accounting for about 8% in terms of mortality globally. The present study aims to explore the effect of silencing Astrocyte Elevated Gene-1 (AEG-1), a metastasis mediating factor, and how it interacts with Exostosin-1 (EXT-1) protein to inhibit the proliferative and invasive potential in colon cancer cells. Forward siRNA transfection was performed using AEG-1 siRNA in SW480 and SW620 colon cancer cell lines, and the expression levels of mRNA and protein were analyzed by Real-time PCR and Immunofluorescence. A simple bioinformatics approach was carried out to… More >

HUIFANG CHEN^1,2, XIAOYING ZHOU², ZONGHONG LONG², XIANGLONG TANG², HONG LI^2,*

BIOCELL, Vol.44, No.3, pp. 371-379, 2020, DOI:10.32604/biocell.2020.08893

Abstract UQCRC1 is one of the 10 mitochondrial complex III subunits, this protein has a role in energy metabolism, myocardial protection, and neurological diseases. The upstream mechanism of the UQCRC1 protective effect on cardiomyocytes is currently unavailable. In order to explore the upstream molecules of UQCRC1 and elucidate the protective mechanism of UQCRC1 on cardiomyocytes in more detail, we focused on the nuclease-sensitive elementbinding protein 1 (YB-1). We hypothesized YB-1 acts as an upstream regulatory molecule of UQCRC1. This study found that YB-1 RNAi significantly reduces the expression of the UQCRC1 protein level (p < 0.05) and More >

Heya QIAN^1,§, Yan YAN^2,§, Zhengjie SHEN¹, Lixian XU¹, Yun ZUO¹, Tao ZHU^3,*, Yanan CHEN^1,*

BIOCELL, Vol.43, No.4, pp. 321-326, 2019, DOI:10.32604/biocell.2019.07992

Abstract The present study aims to explore the effects of p53 and its target gene Rap2B on the autophagy of U2OS cells. U2OS cells were treated with siRNA against p53, Rap2B, and PLCε. Relative expressions of p53, Rap2B, and PLCε were determined using quantitative polymerase chain reaction (qPCR) and Western blotting, respectively. Levels of IP3 in the cells were determined using Enzyme-linked Immunosorbent Assay (ELISA). Levels of Ca²⁺ were detected using Flow cytometry. Fluorescence microscopy was used to observe the autophagy of cells. Knockdown of p53 significantly decreased the expressions of Rap2B protein. Additionally, knockdown of p53 More >

Shu Q. Liu^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 5-7, 2019, DOI:10.32604/mcb.2019.06960

Abstract This article has no abstract. More >