Hui Yao¹, Liqun Wang³, Jia Guo¹, Weimin Liu⁴, Jingjing Li¹, Yingxiao Wang², Linhong Deng^1,*, Mingxing Ouyang^1,2,3,*

Molecular & Cellular Biomechanics, Vol.17, No.3, pp. 101-111, 2020, DOI:10.32604/mcb.2020.09595 - 01 July 2020

Abstract Prostate cancer is the most common cancer among men beyond 50 years old, and ranked the second in mortality. The level of Prostate-specific antigen (PSA) in serum has been a routine biomarker for clinical assessment of the cancer development, which is detected mostly by antibody-based immunoassays. The proteolytic activity of PSA also has important functions. Here a genetically encoded biosensor based on fluorescence resonance energy transfer (FRET) technology was developed to measure PSA activity. In vitro assay showed that the biosensor containing a substrate peptide ‘RLSSYYSGAG’ had 400% FRET change in response to 1 µg/ml… More >

Jun-Ying Wang^*, Xin Jin^*, Xiao-Feng Li^†

Oncology Research, Vol.26, No.1, pp. 95-101, 2018, DOI:10.3727/096504017X14920318811695

Abstract TMPRSS3 belongs to the large type II transmembrane serine protease (TTSP) family, which plays an important role in the development and progression of tumors. However, the function of TMPRSS3 in nasopharyngeal carcinoma (NPC) remains unclear. The present study aimed to examine the impact of TMPRSS3 on the proliferation, migration, and invasion of NPC cells and their potential mechanisms. Our results demonstrated that the expression of TMPRSS3 was obviously upregulated in human NPC tissues and cell lines. Knockdown of TMPRSS3 expression significantly suppressed the proliferation and tumorigenicity of NPC cells in vitro and in vivo. Furthermore, More >