JIANBO ZHOU^1,2,3, FENG WAN^2,4, BIN XIAO⁵, XIN LI¹, CHENG PENG^2,*, FU PENG^1,3,*

Oncology Research, Vol.32, No.5, pp. 943-953, 2024, DOI:10.32604/or.2023.044775

Abstract Breast and lung cancers are the leading causes of mortality and most frequently diagnosed cancers in women and men, respectively, worldwide. Although the antitumor activity of chalcones has been extensively studied, the molecular mechanisms of isoliquiritigenin analog 2', 4', 4-trihydroxychalcone (metochalcone; TEC) against carcinomas remain less well understood. In this study, we found that TEC inhibited cell proliferation of breast cancer BT549 cells and lung cancer A549 cells in a concentration-dependent manner. TEC induced cell cycle arrest in the S-phase, cell migration inhibition in vitro, and reduced tumor growth in vivo. Moreover, transcriptomic analysis revealed that TEC modulated the activity… More > Graphic Abstract

JIYU WANG^1,#, LIUYING YI^2,#, KEKE HUANG¹, YANGYANG WANG¹, HUIPING WANG¹, ZHIMIN ZHAI^1,*

BIOCELL, Vol.48, No.3, pp. 453-462, 2024, DOI:10.32604/biocell.2024.047871

Abstract Background: The primary cause of treatment failure in patients with refractory or relapsed B-cell non-Hodgkin lymphoma (r/r B-NHL) is resistance to current therapies, and therapy-induced senescence (TIS) stands out as a crucial mechanism contributing to tumor drug resistance. Here, we analyzed SENEX/Rho GTPase Activating Protein 18 (ARHGAP18) expression and prognostic significance in doxorubicin-induced B-NHL-TIS model and r/r B-NHL patients, investigating its target in B-NHL cell senescence and the effect of combining specific inhibitors on apoptosis resistance in B-NHL-TIS cells. Methods: Raji cells were transfected with the human SENEX shRNA recombinant lentiviral vector (Sh-SENEX) and the empty vector negative (NC) to… More >

XUEGANG YANG^1,#, XIANHONG XIANG^2,3,#, GUOHUI XU¹, SHI ZHOU³, TIANZHI AN^3,4,*, ZHI HUANG^3,4,*

Oncology Research, Vol.32, No.1, pp. 213-226, 2024, DOI:10.32604/or.2023.030768

Abstract Hepatocellular carcinoma (HCC), a common malignancy worldwide, still lacks effective clinical treatment. The study aimed to investigate the oncogenes that affect the progression of HCC and their possible mechanisms. In our study, we initially confirmed a higher level of PRDX2 in the bile of HCC patients compared to those with choledocholithiasis by 2-DE, LC-MS, and ELISA. Subsequently, we demonstrated the high expression of peroxiredoxin 2 (PRDX2) in HCC based on the TCGA database and clinical sample analysis. Furthermore, PRDX2 overexpression enhanced the viability of HCC cells. And PRDX2 silencing induced senescence of HCC cells. In vivo, knockdown of PRDX2 significantly… More >

Xi Liu^1,2,#,*, Chuxuan Zhao^2,#, Di Wang^3,#, Gen Pan⁴, Xiaonan Ji², Su Gao², Tanxiao Du², Yating Feng², Wenjing Chen²

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2051-2064, 2023, DOI:10.32604/phyton.2023.028196

Abstract Many studies have already shown that dwarfism and moderate delayed leaf senescence positively impact rice yield, but the underlying molecular mechanism of dwarfism and leaf senescence remains largely unknown. Here, using map-based cloning, we identified an allele of DEP2, DDG1, which controls plant height and leaf senescence in rice. The ddg1 mutant displayed dwarfism, short panicles, and delayed leaf senescence. Compared with the wild-type, ddg1 was insensitive to exogenous gibberellins (GA) and brassinolide (BR). DDG1 is expressed in various organs, especially in stems and panicles. Yeast two-hybrid assay, bimolecular fluorescent complementation and luciferase complementation image assay showed that DDG1 interacts… More >

FRANCISCO RAÚL BORZONE^1,*, MARÍA BELÉN GIORELLO¹, LEANDRO MARCELO MARTINEZ², MARÍA CECILIA SANMARTIN^1,3, LEONARDO FELDMAN⁴, FEDERICO DIMASE⁵, EMILIO BATAGELJ⁶, GUSTAVO YANNARELLI³, NORMA ALEJANDRA CHASSEING^1,*

Oncology Research, Vol.31, No.3, pp. 361-374, 2023, DOI:10.32604/or.2023.028104

Abstract Breast cancer is the predominant form of carcinoma among women worldwide, with 70% of advanced patients developing bone metastases, with a high mortality rate. In this sense, the bone marrow (BM) mesenchymal stem/stromal cells (MSCs) are critical for BM/bone homeostasis, and failures in their functionality, transform the BM into a pre-metastatic niche (PMN). We previously found that BM-MSCs from advanced breast cancer patients (BCPs, infiltrative ductal carcinoma, stage III-B) have an abnormal profile. This work aims to study some of the metabolic and molecular mechanisms underlying MSCs shift from a normal to an abnormal profile in this group of patients.… More >

Zhishu Jiang^#, Cong Gan^#, Yulian Liu, Xiaoli Lin, Limei Peng, Yongping Song, Xiaowei Luo, Jie Xu^*

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 665-677, 2023, DOI:10.32604/phyton.2023.025266

Abstract Senescence is the last developmental process in plant, which has an important impact on crop yield and quality. In this study, a stable hereditary early-senescence line BC64 was isolated from the high-generation recombinant inbred lines of 93-11 and Wuyunjing7 (W7). Genetic analysis showed that the premature aging phenotype was controlled by a dominant gene derived from 93-11. By linkage analysis, the gene was primarily mapped in the region between marker B4 and B5 near the centromere of chromosome 4, described as ES(4). Through multiple backcrossing with W7, the near-isogenic line of ES(4) (NIL-ES(4)) was obtained. Compared with wild-type W7, NIL-ES(4)… More >

NADEZHDA PALKINA, EKATERINA SERGEEVA, TATIANA RUKSHA^*

Oncology Research, Vol.29, No.6, pp. 393-400, 2021, DOI:10.32604/or.2022.025816

Abstract Melanoma is one of the most aggressive types of malignant tumors, commonly affecting young individuals. The treatment of metastatic tumors remains obscure due to the resistance of tumor cells to drugs mediated by various mechanisms. The acquisition of a resistant phenotype is associated with both genetic and epigenetic alterations in cancer cells. Therefore, the current study aimed to investigate whether microRNA (miR)-204-5p could promote alterations in the cell cycle and apoptosis of dacarbazine (DTIC)-treated melanoma cells. Quantitative real time PCR showed that transfection of DTIC-treated SK-MEL-2 melanoma cells with miR-204-5p mimics significantly upregulated miR-204-5p. However, flow cytometric analysis revealed that… More >

Xuesong Wang^1,#, Zhihong Wang^1,2,#, Qiuling Yang¹, Sheng Peng³, Mijun Peng^1,*

Journal of Renewable Materials, Vol.10, No.12, pp. 3131-3145, 2022, DOI:10.32604/jrm.2022.020204

Abstract To evaluate the potential anti-aging ability of Eucommia ulmoides, four characteristic components (chlorogenic acid, geniposidic acid, aucubin, quercetin) were selected to assess their effects on H2O2-induced oxidative damage model of human umbilical vein endothelial cell (HUVEC). Oxidative damage indexes, inflammatory factors, cell cycle, cell apoptosis, cell senescence, and their related proteins were analyzed by methyl thiazolyl tetrazolium (MTT) assay, enzyme-linked immunosorbent assay (ELISA), propidium iodide (PI) staining, annexin V-FITC/ PI double staining, SA β-galactosidase staining, and western blotting (WB). The results showed that H2O2- induced cell growth inhibition rate decreased as supplementation with characteristic components when compared to H2O2 group.… More >

DANILA BOBKOV^1,2,3,*, ANASTASIA POLYANSKAYA¹, ANASTASIA MUSORINA¹, GALINA POLJANSKAYA¹

BIOCELL, Vol.46, No.9, pp. 2053-2058, 2022, DOI:10.32604/biocell.2022.019469

Abstract All non-immortalized mesenchymal stem cells have a limited proliferative potential, that is, replicative senescence (RS) is an integral characteristic of the life of all mesenchymal stem cells (MSCs). It is known that one of the important signs of RS is a decrease of cell motility, and that violations of migration processes contribute to the deterioration of tissue regeneration. Therefore, the characterization of the properties of the cell line associated with RS is a prerequisite for the effective use of MSCs in restorative medicine. One of the key proteins regulating cell motility is the small GTPase RhoA. The main purpose of… More >

LINGHUI YU^#, RONG SHEN^#, YANAN GUO, YANXUAN GUO, CHEN LI, YANFENG SONG^*, DEGUI WANG^*

BIOCELL, Vol.45, No.3, pp. 761-772, 2021, DOI:10.32604/biocell.2021.014602

Abstract Various factors can induce cell degeneration by altering the phenotype and metabolism of cells. Mitochondria play an essential role in cellular homeostasis and function, rendering aging processes highly associated with mitochondrial function and status. Herein, we describe an aging-prone phenotype of murine skin cells caused by depletion of Rad6B (Ube2b), an E2 ubiquitin-conjugating enzyme. In this study, using Masson’s trichrome, we showed that loss of Rad6B causes physiological structure changes in mouse skin with age. In addition, a combination of western blotting experiments, transmission electron microscopy and employment of immunofluorescence staining revealed that depletion of Rad6B was characterized by an… More >