Qian Tao, Guiqi Han, Bujin Ma, Hongmei Jia, Can Zhao, Wenshang Li, Zhuyun Yan^*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1297-1310, 2024, DOI:10.32604/phyton.2024.051923

Abstract Fritillaria cirrhosa D. Don (Liliaceae) is an endangered perennial bulbous plant and its dry bulb is a valuable medicinal material with antitussive and expectorant effects. Nevertheless, lack of resources and expensive prices make it difficult to meet clinical needs. This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F. cirrhosa, focusing on: (1) callus induction, (2) bulblets and adventitious bud induction, and (3) artificial seed production. Callus development was achieved in 84.93% on Murashige and Skoog (MS) medium fortified with 1.0 mg·L picloram. The optimal medium for callus differentiation into regenerated… More >

Yuan Tian, Lulu Zhi, Ping Li^*, Xiangyang Hu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1101-1116, 2024, DOI:10.32604/phyton.2024.050220

Abstract Seed viability is an essential feature for genetic resource conservation as well as sustainable crop production. Long-term storage induces seed viability deterioration or seed aging, accompanied by the accumulation of toxic reactive oxygen species (ROS) to suppress seed germination. Controlled deterioration treatment (CDT) is a general approach for mimicking seed aging. The transcription factor ANAC089 was previously reported to modulate seed primary germination. In this study, we evaluated the ability of ANAC089 to control seed viability during aging. Compared with that in the wild-type line, the mutation of ANAC089 significantly increased HO, thereby reducing seed viability after… More >

Md. Parvez Anwar^*, Masuma Akhter, Sharmin Aktar, Sinthia Afsana Kheya, A. K. M. Mominul Islam^*, Sabina Yeasmin, Ahmed Khairul Hasan, Md. Harun Or Rashid

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1159-1177, 2024, DOI:10.32604/phyton.2024.049073

Abstract Seed priming is a pre-germinated technique that can enhance seed germination percentage, faster and synchronized germination, better seedling growth, and yield under stress conditions. To ascertain the most effective seed priming method that would ensure the potential yield of wheat in Bangladesh, two experiments were carried out from December 2021 to March 2022 at the Department of Agronomy, Bangladesh Agricultural University. Two wheat varieties namely BARI Gom-28 and BWMRI Gom-1 were subjected to a range of priming chemicals in both lab and pot tests. These compounds included the following: control (no priming), hydropriming (distilled water),… More >

Xin Zou¹, Jieyu Zhang¹, Ting Cheng¹, Yangyang Guo¹, Xiao Han¹, Han Liu¹, Yuxing Qin¹, Jie Li², Dabing Xiang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.4, pp. 699-712, 2024, DOI:10.32604/phyton.2024.048469

Abstract To mitigate the wastage of seed resources and reduce the usage of pesticides and fertilizers, seed coating agents have gained popularity. This study employs single-factor and multi-index orthogonal experimental design methods to investigate the seed coating formula and physical properties of Tartary buckwheat. The specific effects of each component on Tartary buckwheat seed germination are analyzed. The findings reveal that the seed coating agent formulated with 1.5% polyvinyl alcohol, 0.15% sodium alginate, 0.2% op-10, 0.1% polyacrylamide, 8% colorant, 3% ammonium sulfate, 1% potassium dihydrogen phosphate, and 0.15% carbendazim exhibits the most effective coating. It demonstrates… More >

Wenqing Xiao^1,#, Yuhang Liu^2,#, Yuxin He¹, Qiaoguang Li^1,*, Yongquan Li^3,*

Journal of Renewable Materials, Vol.12, No.3, pp. 615-628, 2024, DOI:10.32604/jrm.2023.046780

Abstract Plasticizers are essential additives in the processing of polyvinyl chloride (PVC), with phthalate plasticizers being widely used. However, these conventional plasticizers have been shown to be harmful to human health and environmentally unfriendly, necessitating the exploration of eco-friendly bio-based alternatives. In this study, Camellia oleifera seed oil, a specialty resource in China, was utilized as a raw material and reacted with 4,4′-Methylenebis(N,N-diglycidylaniline) (AG-80) to synthesize Phenyl Camellia seed Oil Ester (PCSOE). PCSOE was employed as a plasticizer to prepare modified PVC films with varying concentrations, with the conventional plasticizer dioctyl phthalate (DOP) serving as a control.… More >

Haoxue Wu^#, Xiaohan Zhang^§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321

Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression… More >

Rui Zhang¹, Taixiang Chen¹, Zhenjiang Chen¹, Hao Chen¹, Xuekai Wei¹, Malik Kamran¹, Chunjie Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 567-580, 2024, DOI:10.32604/phyton.2024.047485

Abstract In a greenhouse experiment, the effects of soil microorganisms and extracts of Achnatherum inebrians on the seed germination and seedling growth of Elymus nutans were studied. The results showed that both the extracts from aboveground and belowground parts of A. inebrians significantly inhibited the germination rate, germination potential, germination index, vigor index, seedling height, root length, and fresh weight of E. nutans, but increased malondialdehyde content, catalase, peroxidase and superoxide dismutase activity of E. nutans seedlings (p < 0.05). The allelopathy of aqueous extracts of the aboveground parts of A. inebrians was stronger than that of the precipitates. Aqueous extracts of the… More >

Mir Abdullatif Yahya¹, Fatma Tunali¹, Dilek Killi^2,*, Atalay Sökmen²

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 427-444, 2024, DOI:10.32604/phyton.2024.046271

Abstract An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller, a medicinal aromatic plant that is a prominent source of volatile organic compounds (VOCs). Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators (PGRs), and the growth parameters of the nodal segments were examined. Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine (BAP). The superior hormonal concentration of Murashige and Skoog (MS) media for the proliferation of shoots from callus cultures (39.33%) was 5 mg/L of 2-Isopentenyl adenine (2iP), and the favorable media for the growth… More >

Perla Patricia Ochoa-García¹, Jaime Sánchez-Salas², Ricardo Trejo-Calzada¹, Jesús Josafath Quezada-Rivera², Fabián García-González^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 371-386, 2024, DOI:10.32604/phyton.2024.048338

Abstract The genus Argemone L. (Papaveraceae) is found widely distributed in Mexico’s Chihuahuan Desert (CD). Some species of this genus are of phytochemical or ethnobotanical interest. They are inedible plants considered as scrubs. To date they have not been broadly studied; thus, their ecology is, to our knowledge, unknown. The present work was centered around carrying out a morphometric analysis and the determination of minerals in the soil and seeds of the wild populations of Argemone at sites belonging to two ecoregions of the CD in Mexico. In April 2021 and April 2022, seeds of Argemone spp., and… More >

Yong Zhao^2,#, Lili Li^1,#, Rui Luo¹, Mu Peng¹, Tongtong Jiang¹, Mingda Yin¹, Yanpeng Wen¹, Zihan Wang¹, Fenglan Huang^1,4,5,6,7,*, Fanjuan Meng^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.12, pp. 3237-3250, 2023, DOI:10.32604/phyton.2023.031495

Abstract Castor, scientifically known as Ricinus communis L., is among the top ten oil crops globally. It is considered a renewable resource and is commonly referred to as ‘green oil’. Castor seeds contain castor oil as their main component, which is predominantly composed of ricinoleic acid. This study utilized RNAi technology to silence the NPC6 gene in NO.2129 castor, resulting in the creation of mutant plants L1 and L2. The weight of 100 dry seed kernels from L1 and L2 exceeds that from NO.2129. The crude fat and ricinoleic acid levels of L1 and L2 were higher than… More >