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  • Open Access

    ARTICLE

    The Cotton GhWRKY91 Gene Negatively Regulates Root Elongation in Overexpressed Transgenic Arabidopsis thaliana

    Yueying Liu#, Yuqing Wang#, Lijiao Gu*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2937-2946, 2023, DOI:10.32604/phyton.2023.043306 - 24 October 2023

    Abstract WRKY transcription factors play important roles in plant growth, development, and stress responses. Our previous research has shown that the GhWRKY91 gene can delay age-, abscisic acid (ABA)-, and drought-induced leaf senescence when overexpressed in transgenic Arabidopsis plants. To explore in more depth the biological functions of the GhWRKY91 gene, we further observed the root growth of overexpressing transgenic Arabidopsis thaliana under ABA and drought treatment. In this study, we transplanted the germinated seeds of wild-type (WT) and three transgenic lines (OE-12, OE-13 and OE-20) to 1/2 MS solid medium containing ABA and different concentrations of mannitol (simulated… More >

  • Open Access

    ARTICLE

    Overexpression of the LcPIN2 and LtPIN2 Gene in Arabidopsis thaliana Promotes Root Elongation

    Zijian Cao#, Guoxia Xue#, Lingfeng Hu, Haoxian Qu, Shuang Liang, Jisen Shi, Jinhui Chen*, Zhaodong Hao*

    Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2383-2397, 2023, DOI:10.32604/phyton.2023.029845 - 25 June 2023

    Abstract The auxin polar transporter, PIN-FORMED 2 (PIN2) plays an important role in root development. However, it remains unclear whether PIN2 genes form two Liriodendron species, L. chinense (LcPIN2) and L. tulipifera (LtPIN2), are both involved in root development and whether and to what extent these two genes diverge in function. Here, we cloned and overexpressed LcPIN2 and LtPIN2 in Arabidopsis thaliana wild-type (WT) and Atpin2 mutant. Phylogenetic and sequence analysis showed a small degree of differentiation between these two Liriodendron PIN2 genes. Tissue-specific gene expression analysis indicated that both Liriodendron PIN2 genes were highly expressed in roots, implying a potential role in root… More >

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