Gaoyin Wu^1,#, Yundou Pang^1,#, Ya Tan¹, Zhongcheng Peng¹, Shuanggui Geng¹, Guanglin Tao², Qiuying Li¹, Shuang Wang¹, Lihong Zhang¹, Quanyan Tian¹, Yingliang Liu^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.10, pp. 2597-2611, 2024, DOI:10.32604/phyton.2024.056875 - 30 October 2024

Abstract To establish an efficient regeneration method for the rare and endangered plant Mussaenda anomala to address problems regarding its reproductive obstacles and scarce populations. In this study, the terminal buds, axillary buds, stem segments with two axillary buds, stem segments with two axillary buds and one terminal bud, and leaves of M. anomala were used as explants. The effects of different explants and disinfection methods, plant growth regulators and substrates on plant regeneration were explored. The following results were obtained: (1) The terminal bud was a suitable explant for M. anomala tissue culture, and the disinfection method utilized… More >

Shijie Lin¹, Zimo Wang¹, Hongbo Zhu², Conghui Wang³, Hongfeng Wang², Dawei Zhang¹, Tianbing Gou¹, Guangdao Bao¹, Ye Luo¹, Huaijiang He¹, Zhonghui Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 277-289, 2024, DOI:10.32604/phyton.2024.046989 - 27 February 2024

Abstract Tilia amurensis is an economically valuable broadleaf tree species in Northeast China. The production of high-quality T. amurensis varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for in vitro propagation of T. amurensis to alleviate a shortage of high-quality T. amurensis seedlings. Here, we established a rapid in vitro propagation system for T. amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture medium for… More >

Yajun Li^1,#, Yumeng Cui^1,#, Bingjie Wang¹, Yue Li¹, Mengmeng Zhang², Silan Dai¹, He Huang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 869-888, 2022, DOI:10.32604/phyton.2022.018659 - 09 December 2021

Abstract Genetic transformation is one of the key steps in the molecular breeding of chrysanthemum, which relies on an optimal regeneration and transformation system. However, the regeneration system of different chrysanthemum cultivars varies, and the regeneration time of most cultivars is long. To screen cultivars with highly efficient regeneration, leaves and shoot tip thin cell layers (tTCL) from eight chrysanthemum cultivars with different flower colors and flower types were cultured on Murashige and Skoog media (MS) supplemented with 1.0–5.0 mg L⁻¹ 6-benzylaminopurine (6-BA) and 0.1–1.0 mg L⁻¹ α-naphthaleneacetic (NAA). The results showed that the most efficient regeneration… More >

Shidan Chi¹, Tao Luan^1,*, Yan Liang², Xundong Hu², Yan Gao^3,*

FDMP-Fluid Dynamics & Materials Processing, Vol.17, No.1, pp. 71-80, 2021, DOI:10.32604/fdmp.2021.013178 - 09 February 2021

Abstract Improving the primary steam parameters is one of the most direct ways to improve the cycle efficiency of a power generation system. In the present study, the typical problem connected to the excessively high superheat degree of extraction steam in an ultra-supercritical (USC) double-reheat unit is considered. Using a 1000 MW power plant as an example, two systems (case 1 and case 2) are proposed, both working in combination with a regenerative steam turbine. The thermal performances of these two systems are compared with that of the original system through a heat balance method and More >

Si ran Wang^#, Jia Yan^#, Bu er Ha, Yu’e Bai^*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 595-604, 2021, DOI:10.32604/phyton.2021.013693 - 07 February 2021

Abstract Hydrangea bretschneideri Dipp is a highly popular ornamental plant for garden decoration. Genetic engineering technology has been successfully used in many plant species, but it is limited in Hydrangea. Here we established an efficient regeneration system by using stem segments as explants for the first time. In our study, the plant growth regulators (PGRs) were evaluated at the different regeneration processes, including axillary shoots regeneration and root induction. We found that the optimal concentration for axillary buds’ induction was 2.0 mgL⁻¹ 6-BA and 0.5 mgL⁻¹ 1 IAA, its highest induction rate was 70%. Moreover, the highest axillary shoots More >