MAHER KURDI^1,*, ALAA ALKHOTANI², ABDULRAHMAN SABBAGH³, EYAD FAIZO⁴, AHMED I. LARY⁵, AHMED K. BAMAGA⁶, MAJID ALMANSOURI⁷, BADR HAFIZ⁸, THAMER ALSHARIF⁹, SALEH BAEESA⁸

Oncology Research, Vol.32, No.6, pp. 1037-1045, 2024, DOI:10.32604/or.2024.051112 - 23 May 2024

Abstract Background: The dysregulation of Isocitrate dehydrogenase (IDH) and the subsequent production of 2-Hydroxyglutrate (2HG) may alter the expression of epigenetic proteins in Grade 4 astrocytoma. The interplay mechanism between IDH, O-6-methylguanine-DNA methyltransferase (MGMT)-promoter methylation, and protein methyltransferase proteins-5 (PRMT5) activity, with tumor progression has never been described. Methods: A retrospective cohort of 34 patients with G4 astrocytoma is classified into IDH-mutant and IDH-wildtype tumors. Both groups were tested for MGMT-promoter methylation and PRMT5 through methylation-specific and gene expression PCR analysis. Inter-cohort statistical significance was evaluated. Results: Both IDH-mutant WHO grade 4 astrocytomas (n = 22, 64.7%) and IDH-wildtype… More > Graphic Abstract

Haoxue Wu^#, Xiaohan Zhang^§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321 - 28 March 2024

Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression… More >

Jialei Guo^1,2, Fengzhen Li², Guomin Shi³, Weimin Zhao², Tao He^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 343-353, 2024, DOI:10.32604/phyton.2024.047429 - 27 February 2024

Abstract Anthocyanins, ubiquitous in the Compositae family, are regulated by MYB (v-myb avian myeloblastosis viral oncogene homolog), playing an important role in anthocyanin synthesis. In this study, we analyzed the regulation pathway in which the MYB protein of subgroup 6 promotes dihydroflavonol reductase (DFR) expression in Compositae, and validated this law in Saussurea medusa through yeast one-hybrid experiments. Our results showed that MYB and DFR underwent purification selection, DFR promoter analysis revealed the presence of MYB binding site (GAGTTGAATGG) and bHLH binding site (CANNTG) at the sense strand of 84–116 nucleotide residues from the start codon. These two More >

Ji-Yang Zuo^1,2, Huan-Xin Chen^1,2, Zhi-Gang Liu^1,2, Qin Yang^1,2, Guo-Wei He^1,2,*

Congenital Heart Disease, Vol.18, No.1, pp. 7-21, 2023, DOI:10.32604/chd.2022.025451 - 09 January 2023

Abstract Background: Atrial septal defect (ASD) is one of the common congenital heart diseases. The MYH6 gene has a critical role in cardiac development but the role of MYH6 promoter variants in patients with ASD has not been explored. Methods: In 613 subjects including 320 ASD patients, we investigated the MYH6 gene promoter variants and verified the effect on gene expression by using cellular functional experiments and bioinformatics analysis. Results: Eleven variants were identified in the MYH6 gene promoter, of which four variants were found only in ASD patients, and two variants (g.3434G>C and g.4524C>T) were identified for the first… More > Graphic Abstract

YOULIN TUO, XUBAO LIU^*

BIOCELL, Vol.47, No.2, pp. 319-328, 2023, DOI:10.32604/biocell.2023.025250 - 18 November 2022

Abstract Retinoic acid receptor responder 3 (RARRES3) has been characterized as a tumor suppressor in multiple types of cancer. This study aimed to examine the expression profile of RARRES3 across the PAM50 subtypes of breast cancer. The DNA methylation status of RARRES3 was checked in the basal-like subtype, and the underlying mechanisms of its dysregulation were explored. RNA-sequencing (seq) and methylation data from The Cancer Genome Atlas were used for in-silico analysis. Basal-like representative SUM149 and MDA-MB-468 cell lines were used for in vitro and in vivo studies. Compared to tumor-adjacent normal tissues, only the basal-like tumor tissues had… More >

López-Valenzuela BE^1,2, Armenta-Bojórquez AD³, Hernández-Verdugo S⁴, Apodaca- Sánchez MA¹, Samaniego-Gaxiola JA⁵, Valdez-Ortiz A⁶

Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 37-46, 2019, DOI:10.32604/phyton.2019.04621

Abstract Microbes that are beneficial to plants are used to enhance the crop growth, yield and are alternatives to chemical fertilizers. Trichoderma and Bacillus are the predominant plant growth-promoting fungi and bacteria. The objective of this study was select, characterize, and evaluate isolates of Trichoderma spp. and Bacillus spp. native from the northern region of Sinaloa, Mexico, and assess their effect on growth promotion in maize (Zea mays L.). In greenhouse conditions, four Trichoderma isolates and twenty Bacillus isolates, as well as two controls, were tested in a completely randomized design with three replicates. We selected… More >

Hong TIAN^{1, 2}, Peiyu CONG³, Ruiqun QI⁴, Xinghua GAO⁵, Xin LIU⁶, Hongsheng LIU⁷, Fengping SHAN^1,*

BIOCELL, Vol.41, No.1, pp. 27-32, 2017, DOI:10.32604/biocell.2017.41.027

Abstract Glioma is one of the lethal central nervous system tumors. The infiltrative and invasive growth nature makes it difficult to identify the boundary between glioma and the normal tissues, resulting in inevitable recurrence after surgery operation. Gliomas do not metastasize, so to prevent the residual tumor from proliferating or invading is a key challenge. Previous report indicated that hypotaurine could facilitate glioma invasion and suppress demethylases’ activities. Using a hypotaurine synthesis deficient U251 cell line, we proved that the cells invasion ability was impaired. Gene expression profile analysis exhibited that knocking down one of… More >

Robles-Hernández L, J Hernández-Huerta, AC González-Franco, OA Hernández-Rodríguez, A Núñez-Barrios, R Pérez-Leal

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 253-261, 2015, DOI:10.32604/phyton.2015.84.253

Abstract Chili pepper is one of the main crops of economic importance in Mexico, and Fusarium wilting is a disease that limits its production. In addition, the inappropriate use of agrochemicals in farming activities generate environmental and health problems. Therefore, in this study the effectiveness of Streptomyces sp PRIO41 was evaluated as a (1) biocontrol agent of Fusarium spp and (2) plant growth promoter bacteria. Assays of pathogenicity and virulence of Fusarium spp. in jalapeño pepper seeds, and interactions of these pathogens with Streptomyces PRIO41 were evaluated under two nutritional conditions. In the greenhouse, the effectiveness of Streptomyces sp.… More >

Hernández-Castillo FD¹, RH Lira-Saldivar², G Gallegos-Morales¹, M Hernández-Suárez¹, S Solis-Gaona²

Phyton-International Journal of Experimental Botany, Vol.83, pp. 49-55, 2014, DOI:10.32604/phyton.2014.83.049

Abstract One of the most severe phytosanitary problems that face chili pepper producers in Mexico, and in many other parts of the world, is the disease known as "secadera" or wilting, caused by diverse pathogens. These patogens are mainly controlled with synthetic pesticides, thus causing a severe ecological impact, toxicity to humans, generation of plant resistance to fungicides, and increments of production costs. Because of this, it rises the need of finding more environmentally friendly options. We evaluated rhizospheric bacteria as a possible biological control of pepper wilt. We used three bacterial strains belonging to the Bacillus More >

VALERIA C. DENNINGHOFF^1,2*, GEORGINA P. OSSANI¹, ANA M. UCEDA¹, MARIA A. AVAGNINA², BORIS ELSNER², ALBERTO J. MONSERRAT¹

BIOCELL, Vol.34, No.2, pp. 65-70, 2010, DOI:10.32604/biocell.2010.34.065

Abstract Weanling Sprague-Dawley rats were fed on a choline-deficient diet with hydrogenated vegetable oil and corn oil as lipids develop acute renal failure. Pathogenesis of the latter is controversial and an ischemic mechanism has been proposed. Arachidonic acid derivatives are involved in the regulation of vascular tonus. Vasospasm could be due to an increase in tromboxane A2-mediated vasoconstriction or to a decrease in prostacyclin-induced vasodilatation. Enzymes involved in the synthesis of both compounds are tromboxane A2- and prostacyclin-synthase respectively. The aim of this study was to identify the variable number tandem repeats (VNTR) in the promoter More >