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  • Open Access

    ARTICLE

    Genetic Diversity, Population Structure, and Genome-Wide Association Study of Seven Agronomic Traits in 273 Diverse Upload Cotton Accessions

    Yajun Liang1,2,#, Juyun Zheng1,#, Junduo Wang1,#, Zhaolong Gong1, Zhiqiang Li3, Ling Min4, Zeliang Zhang2, Zhiwei Sang2, Yanying Qu2, Xueyuan Li1,*, Quanjia Chen2,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.12, pp. 3345-3357, 2023, DOI:10.32604/phyton.2023.028755 - 28 December 2023

    Abstract Upland cotton (Gossypium hirsutum) is the most important plant producing natural fibers for the textile industry. In this study, we first investigated the phenotypic variation of seven agronomic traits of 273 diverse cotton accessions in the years 2017 and 2018, which were from 18 geographical regions. We found large variations among the traits in different geographical regions and only half of the traits in either years 2017 or 2018 followed a normal distribution. We then genotyped the collection with 81,612 high quality SNPs. Phylogenetic tree and population structure revealed a diverse genetic structure of the core… More >

  • Open Access

    ARTICLE

    Genetic Diversity and Population Structure Analysis of Barley Landraces from Shanghai Region Using Genotyping-by-Sequencing

    Luli Li1,2, Nigel G. Halford3, Huihui Wang4, Yingjie Zong1, Zhenzhu Guo1, Ruiju Lu1, Chenghong Liu1, Zhiwei Chen1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1275-1287, 2023, DOI:10.32604/phyton.2023.026946 - 06 January 2023

    Abstract Barley (Hordeum vulgare L.) is an important economic crop for food, feed and industrial raw materials. In the present research, 112 barley landraces from the Shanghai region were genotyped using genotyping-by-sequencing (GBS), and the genetic diversity and population structure were analyzed. The results showed that 210,268 Single Nucleotide Polymorphisms (SNPs) were present in total, and the average poly-morphism information content (PIC) was 0.1642. Genetic diversity and population structure analyses suggested that these barley landraces were differentiated and could be divided into three sub-groups, with morphological traits of row-type and adherence of the hulls the main distinguishing More >

  • Open Access

    ARTICLE

    Population Structure Analysis and Genome-Wide Association Study of Tea (Camellia sinensis (L.) K untze) Germplasm in Qiannan, China, Based on SLAFSeq Technology

    Fen Zhang1, Weili Tian1, Lu Cen1, Litang Lv2, Xiaofang Zeng1, Yulu Chen1, Yichen Zhao2,*, Yan Li1,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 791-809, 2022, DOI:10.32604/phyton.2022.018104 - 09 December 2021

    Abstract Duyun Maojian tea is a famous tea in China. In this study, the specific-locus amplified fragment (SLAF) sequencing method was used to analyze the population structure and conduct a genome-wide association study (GWAS) of 2 leaf traits of 123 tea plants in Qiannan, China. A total of 462,019 SLAF tags and 11,362,041 single-nucleotide polymorphism (SNP) loci were obtained. The results of phylogenetic tree analysis, cluster analysis, and principal component analysis showed that 123 tea germplasms were clustered into three groups, and the heterozygosity rates for Groups I, II, and II were 0.206, 0.224, and 0.34,… More >

  • Open Access

    ARTICLE

    Diversity Assessment of an Endemic Carpinus oblongifolia (Betulaceae) Using Specific-Locus Amplified Fragment Sequencing and Implications for Conservation

    Sumei Li, Qing Wang, Peng Wang, Shuan Wang, Rutong Yang, Ya Li*

    Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 617-632, 2022, DOI:10.32604/phyton.2022.017741 - 26 October 2021

    Abstract Carpinus oblongifolia is an endemic species and the extant wild populations show a fragmentation distribution in the Baohua Mountain of Jiangsu Province in eastern China. Understanding of genetic diversity plays an important role in C. oblongifolia survival and sustainable development. The wild C. oblongifolia population was artificially divided into four subpopulations according to the microhabitats, and another two subpopulations were constructed by progeny seedlings cultivated with the mature seeds. Then, the leaf buds of 80 individuals from six subpopulations were sampled to develop single nucleotide polymorphisms (SNPs) using specific-locus amplified fragment sequencing (SLAF-seq). Based on these SNPs, we… More >

  • Open Access

    ARTICLE

    Development of new cowpea (Vigna unguiculata) mutant genotypes, analysis of their agromorphological variation, genetic diversity and population structure

    MADE DIOUF1, SARA DIALLO1, FRANÇOIS ABAYE BADIANE1,2, OUMAR DIACK1, DIAGA DIOUF1,*

    BIOCELL, Vol.45, No.2, pp. 345-362, 2021, DOI:10.32604/biocell.2021.013706 - 19 February 2021

    Abstract Cowpea is one of the most important legume grains in the sub-Saharan region of Africa used for human consumption and animal feed, but its production is hampered by biotic and abiotic constraints raising the need to broaden its genetic basis. For this purpose, seeds of two cowpea varieties Melakh and Yacine were irradiated with 300 and 340 Gy of gamma-ray, respectively. The developed mutant populations were agromorphologically characterized from M5 to M7, while the genetic diversity of the latter was evaluated using 13 ISSR markers. Based on the agromorphological characterization, variation of flower color, pod… More >

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