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  • Open Access

    ARTICLE

    Phytohormones Accumulation and Distribution in Shoots and Roots of Haploid, Diploid and Tetraploid Barley Seedlings Derived from Microspore Culture

    Longhua Zhou1,2,#, Ting He1,2,#, Jing Li1,2,3, Guimei Guo1,2, Yingbo Li1,2, Hongwei Xu1,2, Runhong Gao1,2, Linli Huang1,2, Yifei Wang1,2, Ruiju Lu1,2, Zhiwei Chen1,2,*, Chenghong Liu1,2,*

    Phyton-International Journal of Experimental Botany, Vol.91, No.7, pp. 1419-1428, 2022, DOI:10.32604/phyton.2022.019912

    Abstract Phytohormones play important roles in plant growth and development, and polyploids are thought to be an important method for plant breeding. However, the relationship between ploidy and phytohormone is still unclear. In this study, barley at three ploidy levels were produced by microspore culture. Therefore, we further analyzed the phytohormone content in the shoots and roots of the three kinds of barley materials to study the effect of ploidy on phytohormones accumulation and distribution. The results showed that Abscisic acid (ABA), gibberellin (GA), jasmonic acid (JA), auxin (IAA), salicylic acid (SA) and cytokinin (CTK) were successfully determined in shoots and… More >

  • Open Access

    ARTICLE

    Cytogenetical Changes among Polyembryonic (PEm) and Non-PEm Maize Plants

    A. A. Román-Calzoncit1, F. Ramírez-Godina2,*, J. Sánchez-Laureano2, A. C. Flores-Gallegos1, J. Espinoza-Velázquez2, R. Rodríguez-Herrera1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 87-97, 2021, DOI:10.32604/phyton.2020.013880

    Abstract Polyembryony in maize (PEm) contributes to improving the nutritional properties of the grain, as well as an increase in yield, since it generates multiple plants per seed, opening the possibility of developing new varieties. However, it is unknown whether polyembryony in maize is the product of chromosomal abnormalities. Based on the above, in this research a cytogenetic study was proposed to verify if chromosomal abnormalities are related to the maize polyembryony. For a meiotic study, maize genotypes with variable proportions of polyembryony (PEm), from the UA-IMM-BAP population and non-PEm (monoembryonic) maize were used, while for a mitosis analysis, 30 families… More >

  • Open Access

    ARTICLE

    Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho & Hiern) Stapf (Gramineae)

    KARINA SAYURI UTSUNOMIYA*, MARIA SUELY PAGLIARINI*, CACILDA BORGES DO VALLE**

    BIOCELL, Vol.29, No.3, pp. 295-301, 2005, DOI:10.32604/biocell.2005.29.295

    Abstract The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho & Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x… More >

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