Riwa Mahai¹, Rongpeng Liu¹, Xiaolang Du¹, Zejing Mu¹, Xiaoyun Wang^1,*, Jun Yuan^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1203-1219, 2024, DOI:10.32604/phyton.2024.051559 - 27 June 2024

Abstract Rosaceae represents a vast and complex group of species, with its classification being intricate and contentious. The taxonomic placement of many species within this family has been a subject of ongoing debate. The study utilized the Illumina platform to sequence 19 plant species from 10 genera in the Rosaceae. The cp genomes, varying in size from 153,366 to 159,895 bp, followed the typical quadripartite organization consisting of a large single-copy (LSC) region (84,545 to 87,883 bp), a small single-copy (SSC) region (18,174 to 19,259 bp), and a pair of inverted repeat (IR) regions (25,310 to… More >

Shugufta Parveen¹, Fayaz A. Mohiddin^2,*, M. Ashraf Bhat³, Zahoor Ahmed Baba⁴, Fehim Jeelani⁵, M. Anwar Bhat⁶, Sajad Un Nabi⁷, Burhan Hamid², Saba Bandey⁸, Farhanaz Rasool⁹, Zakir Amin¹, Ibrahim Al-Ashkar^10,*, Muhammad Adnan¹¹, Ayman El Sabagh¹²

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3021-3041, 2023, DOI:10.32604/phyton.2023.030921 - 24 October 2023

Abstract One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL⁻¹) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM… More >

XIAOJUN ZHOU^1,*, MENGXUE LIU¹, LINLIN SONG²

BIOCELL, Vol.47, No.3, pp. 657-668, 2023, DOI:10.32604/biocell.2023.026781 - 03 January 2023

Abstract Azalea is a general designation of Rhododendron in the Ericaceae family. Rhododendron not only has high ornamental value but also has application value in ecological protection, medicine, and scientific research. In this study, we used Illumina and PacBio sequencing to assemble and annotate the entire chloroplast genomes (cp genomes) of four Rhododendron species. The chloroplast genomes of R. concinnum, R. henanense subsp. lingbaoense, R. micranthum, and R. simsii were assembled into 207,236, 208,015, 207,233, and 206,912 bp, respectively. All chloroplast genomes contain eight rRNA genes, with either 88 or 89 protein-coding genes. The four cp genomes were compared and analyzed by More >

Qianqian Li¹, Qi Zhao^1,2,*, Xinzhuan Yao¹, Baohui Zhang¹, Litang Lu^1,2,*

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2163-2189, 2022, DOI:10.32604/phyton.2022.021530 - 30 May 2022

Abstract The cellulose synthase gene superfamily, including Cellulose synthase A (CesA) and cellulose synthase-like (Csl) gene families, is responsible for the synthesis of cellulose and hemicellulose, respectively. The CesA/Csl genes are vital for abiotic stress resistance and shoot tenderness regulation of tea plants (Camellia sinensis). However, the CesA/Csl gene family has not been extensively studied in tea plants. Here, we identified 53 CsCesA/Csl genes in tea plants. These genes were grouped into five subfamilies (CsCesA, CsCslB, CsCslD, CsCslE, CsCslG) based on the phylogenetic relationships with Arabidopsis and rice. The analysis of chromosome distribution, gene structure, protein domain and motif revealed that most genes in CsCesA,… More >

Sadia Nadir^1,2,3, Sehroon Khan¹, Dhanushka N. Wanasinghe^1,2, Saowaluck Tibpromma^1,2, Shahid Iqbal^1,2, Jianchu Xu^1,2,*, Peter Mortimer^1,2,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1031-1039, 2021, DOI:10.32604/phyton.2021.012075 - 30 March 2021

Abstract During a survey of soil fungi in Yunnan Province, several isolates of Aspergillus were obtained. Based on morphology and molecular analyses of internal transcribed spacer regions and intervening 5.8S nrRNA gene (ITS), β-tubulin and calmodulin (CaM) genes sequences, two isolates were identified as Aspergillus vadensis (section Nigri). A phylogenetic tree, detailed descriptions, illustrations and scanned electron microscopy morphology are provided for the new isolates. This is the first record of A. vadensis from China. More >

Thatsanee Luangharn^1,2,3,4, Samantha C. Karunarathna^1,3, Peter E. Mortimer^1,4,*, Kevin D. Hyde^3,5, Jianchu Xu^1,3,4,*

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 743-764, 2020, DOI:10.32604/phyton.2020.09690 - 22 June 2020

Abstract Ganoderma is a genus of medicinally and economically important mushrooms in the family Ganodermataceae. Ganoderma species are popular medicinal mushrooms and their health benefits are well-documented. Ganoderma is a cosmopolitan genus that is widely distributed in both tropical and temperate regions. This genus is characterized by its unique laccate or non-laccate species with double-walled basidiospores. Here, we report on eight collections of G. gibbosum collected during surveys in Kunming, Yunnan Province, China. The specimens are described and illustrated based on macro- and micro-morphological characteristics. Total DNA of the eight G. gibbosum strains were extracted using the Biospin Fungal More >

Jutamart Monkai¹, Ruvishika S. Jayawardena^1,2, Sajeewa S. N. Maharachchikumbura^3,*, Kevin D. Hyde¹

Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 765-777, 2020, DOI:10.32604/phyton.2020.09720 - 22 June 2020

Abstract We isolated an interesting fungus from dead leaves of Magnolia liliifera collected from Chiang Mai, Thailand. The novel strain is related to Plectosphaerellaceae based on the morphology of its asexual morph and the analysis of sequence data. Phylogenetic analyses using a combined gene analysis of LSU and ITS sequence data showed that this strain is clustered in the same clade with Furcasterigmium furcatum with high statistical support. The new strains produced the asexual morph in culture which is morphologically similar to F. furcatum. Thus, we identified this strain as the sexual morph of F. furcatum. This is the first More >

Farshid GOLSHANI1, Barat Ali FAKHERI¹, Mahmood SOLOUKI¹, Nafiseh MAHDINEZHAD¹, Majid Reza KIANI FERIZ²

BIOCELL, Vol.43, No.3, pp. 225-232, 2019, DOI:10.32604/biocell.2019.07547

Abstract ITS (Internal Transcription Spacer of nuclear ribosomal DNA) regions method was applied to identify and study the phylogenetic relationships existing between 41 individuals of Crocus, including 8 species collected in Iran (4 crop and 8 wild individuals) and 16 species (2 crop and 27 wild individuals) already registered in GenBank (NCBI). PCR amplifications were performed using the primer designed on the nucleotide sequence of nuclear region (ITS). A total of 12 sequences were obtained and registered in NCBI database. In particular, 4 of these sequences were not already present in the scientific library. Nucleotide… More >

ISRAEL A. VEGA^*1,2, FEDERICO A. DELLAGNOLA¹, JORGE A. HURST³, MARTÍN S. GODOY¹ AND ALFREDO CASTRO-VAZQUEZ^1,2

BIOCELL, Vol.36, No.2, pp. 47-55, 2012, DOI:10.32604/biocell.2012.36.047

Abstract Pigments present in the brown-greenish C morph of an intracellular endosymbiont of Pomacea canaliculata were investigated. Acetone extracts of the endosymbiotic corpuscles showed an absorption spectrum similar to that of chlorophylls. Three fractions obtained from silica gel column chromatography of the acetone extracts (C_I , C_II and C_III ), were studied by positive ion fast atom bombardment-mass spectrometry (FAB–MS) and hydrogen-nuclear magnetic resonance (H-NMR). Results indicated the presence of (1) a sterol in the yellow colored C_I fraction; (2) a mixture of pheophorbides a and b in the major green fraction, C_II; and (3) a modified pheophorbide a in the More >

Deng JB¹, CB Ding¹, L Zhang¹, YH Zhou², RW Yang¹

Phyton-International Journal of Experimental Botany, Vol.80, pp. 181-188, 2011, DOI:10.32604/phyton.2011.80.181

Abstract Four isozymes, superoxide dismutase (SOD), polyphenol oxidase (PPO), malate dehydrogenase (MDH) and cytochrome oxidase (COD) were studied for identification of six herbal species (Curcuma L.). All the 37 study specimens produced a total of 168 polymorphism isozyme bands. The genetic distance coefficients (GS) varied from 0.08 to 0.54. The dendrogram, obtained according to the polymorphism isozyme bands by the UPGMA method with the software NTSYS–pc2.1, contributed to improve the resolution of phylogeny. From the dendrogram, it was possible to differentiate between the wild and cultivated specimens of C. longa, and within C. sichuanensis species. More >