Olha Mykhailenko^1,2,3,#,*, Zigmantas Gudžinskas⁴, Liudas Ivanauskas⁵, Victoriya Georgiyants¹, Chia-Hung Yen^6,7,8, Chung-Fan Hsieh⁹, Riong Seulina Panjaitan⁶, Tsong-Long Hwang^10,11,12,13, Bing-Hung Chen^14,15, Michal Korinek^6,7,8,#,*

Phyton-International Journal of Experimental Botany, Vol.95, No.1, 2026, DOI:10.32604/phyton.2026.074209 - 30 January 2026

Abstract The sustainable sourcing of novel bioactive compounds from natural sources is crucial to the success of the pharmaceutical, food, and cosmetics industries. Iris bucharica Foster (syn. Juno bucharica (Foster) Vved.) is a promising source of novel bioactive molecules, particularly phenolic compounds, which are renowned for their antioxidant properties. In this study, we developed a reliable HPLC-UV-DAD method to identify and quantify phenolic compounds in the leaves and bulbs of I. bucharica, establishing the first set of quality control markers for this species. A total of 21 phenolic compounds were identified in the leaves, with flavonoids isoorientin, guaijaverin, hyperoside, More >

Oleh Koshovyi¹, Amal D. Premarathna², Rando Tuvikene², Valdas Jakštas^3,4, Vaidotas Žvikas³, Yurii Hrytsyk⁵, Lyubov Grytsyk⁵, Andriy Grytsyk⁵, Jaan Liira⁶, Ain Raal^1,*

Phyton-International Journal of Experimental Botany, Vol.94, No.12, pp. 3991-4019, 2025, DOI:10.32604/phyton.2025.074153 - 29 December 2025

Abstract Gilia capitata Sims (Polemoniaceae) is an annual herbaceous plant widely distributed in western North America and cultivated as an ornamental flower. The detailed chemical composition and pharmacological properties of this plant have not been previously reported. This study aimed to investigate the chemical composition and pharmacological activity of aqueous-ethanolic extracts derived from different plant parts (herb, flowers, stems, roots, and fruits). HPLC analysis identified 12 phenolic compounds and 16 amino acids in the aqueous-ethanolic extracts obtained from these plant parts. The biological effects of the extracts were evaluated in vitro, including cytotoxicity, anti-inflammatory response, and wound-healing potential.… More >

Lucija Galić, Zdenko Lončarić, Miroslav Lisjak^*

Phyton-International Journal of Experimental Botany, Vol.94, No.11, pp. 3297-3318, 2025, DOI:10.32604/phyton.2025.072504 - 01 December 2025

Abstract Phenolic compounds represent a broad and structurally diverse class of plant secondary metabolites with importance for both plant biology and human health. This review provides a comprehensive overview of their biosynthesis, chemical diversity, multifaceted functions in plants, roles in the wider ecosystem, and significance in human nutrition and biotechnology. Primarily synthesized via the phenylpropanoid pathway, these compounds encompass major classes such as lignin, flavonoids, and tannins. Within the plant, they perform critical functions including providing structural support (lignin), defending against biotic stresses (e.g., pathogens, herbivores), mediating ecological interactions (pollination, symbiosis, allelopathy), and protecting against abiotic… More >

Ahmed Mahmoud Ismail^1,*, Eman Said Elshewy², Ayman Y. Ahmed³, Hossam M. Darrag⁴

Phyton-International Journal of Experimental Botany, Vol.94, No.10, pp. 3023-3045, 2025, DOI:10.32604/phyton.2025.067444 - 29 October 2025

Abstract Powdery mildew of cucumber (Cucumis sativus L.) is a destructive disease caused by Podosphaera xanthii (Castagne) U.Braun & Shishkoff. This study aimed to investigate the antifungal effect of extracts of Ulva lactuca, Spirulina platensis, and Nostoc muscorum against P. xanthii and to improve the physiological and morphological traits of cucumber under commercial greenhouse conditions. The chemical composition of the individual extracts from U. lactuca, S. platensis, and N. muscorum was analyzed utilizing High-performance Liquid Chromatography (HPLC) and Gas Chromatography/Mass spectrometry (GC/MS). Cucumber plants were sprayed twice with 5% of the crude extracts of U. lactuca, S. platensis, and N. muscorum and their mixture (U. lactuca, S. platensis, and N. muscorum).… More >

Lesya Voytenko^1,*, Mykola Shcherbatiuk¹, Valentyna Vasyuk¹, Kateryna Romanenko¹, Lidiya Babenko¹, Oleksandr Smirnov^2,3, Iryna Kosakivska¹

Phyton-International Journal of Experimental Botany, Vol.94, No.7, pp. 2195-2214, 2025, DOI:10.32604/phyton.2025.067772 - 31 July 2025

Abstract Prolonged lack of rain and high-temperature lead to soil water deficits, inhibiting cereal crop growth in early ontogenesis and reducing grain quality and yield. Rye (Secale cereale L.) is a key grain crop, particularly in regions where wheat cultivation is challenging or unfeasible. To clarify its drought adaptation mechanisms, we analyzed the effects of moderate soil drought on growth, hormonal homeostasis, and the dynamics and distribution of free amino acids and phenolic compounds in rye at early vegetative stages and post-recovery. Drought triggered both general and organ-specific changes in endogenous phytohormones. A nonspecific response involved the… More >

Lucas Leal Lima¹, Angélica Souza Gouveia¹, Analice Martins Duarte¹, Filipe Schitini Salgado², Nathália Silva Oliveira¹, Monique da Silva Bonjour¹, Iana Pedro da Silva Quadros¹, Maria Goreti Almeida Oliveira¹, Flavia Maria Silva Carmo², Elizabeth Pacheco Batista Fontes¹, Humberto Josué de Oliveira Ramos^1,3,*

Phyton-International Journal of Experimental Botany, Vol.94, No.6, pp. 1787-1810, 2025, DOI:10.32604/phyton.2025.064068 - 27 June 2025

Abstract Sound contains mechanical signals that can promote physiological and biochemical changes in plants. Insects produce different sounds in the environment, which may be relevant to plant behavior. Thus, we evaluated whether signaling cascades are regulated differently by ecological sounds and whether they trigger molecular responses following those produced by herbivorous insects. Soybean plants were treated with two different sounds: chewing herbivore and forest ambient. The responses were markedly distinct, indicating that sound signals may also trigger specific cascades. Enzymes involved in oxidative metabolism were responsive to both sounds, while salicylic acid (SA) was responsive only… More >

Wissal Benaffari^1,2,3, Fatima-Ezzahra Soussani^1,2,4, Abderrahim Boutasknit^1,2,5, Salma Toubali^1,2,3, Abir Ben Hassine³, Hala Ben Ahmed³, Rachid Lahlali^7,*, Abdelilah Meddich^1,2,6,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2285-2302, 2024, DOI:10.32604/phyton.2024.055052 - 30 September 2024

Abstract Quinoa (Chenopodium quinoa) has recently gained popularity as a pseudo-cereal cultivated in various countries due to the nutritional and antioxidant benefits of its seeds, and its capacity to persist in water-stressed environments. Our study aimed to assess the effects of native arbuscular mycorrhizal fungi (AMF) and local organic amendments on the metabolic responses and antioxidant activity of quinoa seeds under water-stressed conditions. To this end, quinoa plants were grown in soils inoculated with an indigenous mycorrhizal consortium AMF and amended with two types of compost from horse manure (HM) and green waste (GW) under two water… More >

Yolotzin Apatzingán Palomino-Hermosillo¹, Ángel Elpidio Díaz-Jasso², Rosendo Balois-Morales¹, Verónica Alhelí Ochoa-Jiménez^1,3, Pedro Ulises Bautista-Rosales¹, Guillermo Berumen-Varela^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.7, pp. 1717-1732, 2024, DOI:10.32604/phyton.2024.052216 - 30 July 2024

Abstract Soursop (Annona muricata L.) is a tropical fruit highly valued for its unique flavor, nutritional value, and health-promoting properties. The ripening process of soursop involves complex changes in gene expression and metabolite accumulation, which have been studied using various omics technologies. Transcriptome analysis has provided insights into the regulation of key genes involved in ripening, while metabolic compound analysis has revealed the presence of numerous bioactive compounds with potential health benefits. However, the integration of transcriptome and metabolite compound data has not been extensively explored in soursop. Therefore, in this paper, we present a comprehensive analysis… More >

Ain Raal^1,*, Linda Rusalepp², Tatiana Chiru³, Nicolae Ciobanu³, Kelly Talvistu¹, Michelle Shusta¹, Oleh Koshovyi^1,4, Tõnu Püssa²

Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2965-2979, 2023, DOI:10.32604/phyton.2023.042723 - 24 October 2023

Abstract The fruits of the Sea buckthorn (Hippophae rhamnoides L.) are a popular plant food and a valuable health product. Cultivating plants produces a lot of leaves with fine branches as an unnecessary residue, which needs valorization. The aim of the study was to estimate (by HPLC-MS/MS) the qualitative and quantitative content of polyphenolic compounds in different parts of H. rhamnoides (HR), and to determine the level of antioxidant activity of leaves and fruits (by DPPH, ABTS methods and ferrozine test). Up to 19 compounds were identified in different parts of HR. The fruits are rich in flavonoids, including… More >

Oliver Tusevski^1,*, Marija Todorovska¹, Jasmina Petreska Stanoeva², Marina Stefova², Sonja Gadzovska Simic¹

Phyton-International Journal of Experimental Botany, Vol.92, No.3, pp. 921-941, 2023, DOI:10.32604/phyton.2023.024995 - 29 November 2022

Abstract Three Hypericum perforatum hairy root lines (HR B, HR F and HR H) along with non-transformed roots were analyzed for phenolic compounds composition and in vitro enzyme inhibitory properties. In silico molecular modeling was performed to predict the interactions of the most representative phenolic compounds in HR clones with enzymes related to depression, neurodegeneration and diabetes. Chromatographic analyses revealed that HR clones represent an efficient source of quinic acid and hydroxybenzoic acids, epicatechin and procyanidin derivatives, quercetin and kaempferol glycosides, as well numerous xanthones. In vitro antidepressant activity of HR extracts through monoamine oxidase A (MAO-A) inhibition was attributed… More >