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  • Open Access

    Analysis of Subcellular Localization and Pathogenicity of Plum Bark Necrosis Stem-Pitting Associated Virus Protein P6

    Yuanyuan Li1,#, Jinze Mu2,#, Qingliang Li1, Huabing Liu3, Xuefeng Yuan2,*, Deya Wang1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2079-2085, 2023, DOI:10.32604/phyton.2023.028237 - 29 May 2023

    Abstract Infection of plum bark necrosis stem pitting associated virus (PBNSPaV) has been reported in many Prunus species in several countries, causing significant economic losses. The very small proteins encoded by plant viruses are often overlooked due to their short sequences and uncertain significance. However, numerous studies have indicated that they might play important roles in the pathogenesis of virus infection. The role of small hydrophobic protein P6, encoded by the open reading frame 2 of PBNSPaV, has not been well explored. In this study, we amplified the P6 fragment from a PBNSPaV isolate by RT-PCR using specific… More >

  • Open Access

    ARTICLE

    Characterization and Pathogenicity of Pseudopestalotiopsis vietnamensis Causing Gray Blight of Wuyi Rock Tea (Camellia sinensis) in China and Specific Mechanisms of Disease Infection

    Guangheng Wu1,#,*, Lu Rui2,3,#, Xiang Lu4, Libo Han2, Gan Lv1, Xianyu Fu5, Jinxian Liu5, Nong Zhou3, Chuanhai Zhang1

    Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 131-147, 2023, DOI:10.32604/phyton.2022.021919 - 06 September 2022

    Abstract Gray blight disease (GBD) causes significant losses in tea production in China. Although genes and biological processes involved in resistance to fungal disease in tea plants have been identified, specific mechanisms of the GBD infection process remain unknown. In this study, morphological and multi-gene (TEF-TUB-ITS) phylogenetic characteristics were used to identify isolate CLBB1 of Pseudopestalotiopsis vietnamensis. Pathogenicity tests confirmed that isolate CLBB1 from tea leaves caused GBD in the susceptible tea cultivar Wuyi Rock (Camellia sinensis var. sinensis cv. Shuixian). Spores began to germinate 24 h after infection (hai), and after 48 h, elongated fungal hyphae formed from a single More >

  • Open Access

    ARTICLE

    UVI_02019870, a Puptive Effector from Ustilaginoidea virens, Interacts with a Chloroplastic-Like Protein OsCPL1

    Shuai Li, Jianming Zhou, Shibo Xiang, Songhong Wei*

    Phyton-International Journal of Experimental Botany, Vol.89, No.3, pp. 681-690, 2020, DOI:10.32604/phyton.2020.010671 - 22 June 2020

    Abstract Ustilaginoidea virens, which causes rice false smut (RFS), is one of the most detrimental rice fungal diseases and poses a severe threat to rice production and quality. Effectors in U. virens often act as a group of essential virulence factors that play crucial roles in the interaction between host and the pathogen. Thus, the functions of individual effectors in U. virens need to be further explored. Here, we found a small secreted hypersensitive response-inducing protein UVI_02019870 was highly conserved in fungi. Furthermore, we performed Y2H and BiFC assay to demonstrated UVI_02019870 interacted with OsCPL1, which was predicted as More >

  • Open Access

    ARTICLE

    First report of Phytopythium vexans causing the “Avocado sadness” in Michoacan, Mexico

    Hernández Pérez A1, E Cerna Chávez2, JC Delgado Ortiz3, M Beltrán Beache4, LM Tapia Vargas5, YM Ochoa Fuentes2

    Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 11-13, 2019, DOI:10.32604/phyton.2019.04608

    Abstract Mexico is the main producer, consumer and exporter of avocado in the world, being Michoacan the main producer state contributing more than 80% of the national production. There are phytopathogens that decimate the production causing the death of the tree. Root samples were collected in avocado trees that showed the characteristic symptomatology of the disease known as avocado sadness, the sampling was carried out in four of the main avocado producing towns, in the state of Michoacan, Mexico. The isolation consisted in sowing root tissue in Petri dishes with V8®-PARPH culture medium, subsequently they were identified More >

  • Open Access

    ARTICLE

    First morphogenetic identification of Fusarium solani isolated from orange fruit in Egypt

    Abd-Elsalam KA1,2, K Youssef1,2, H Almoammar3

    Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 128-131, 2015, DOI:10.32604/phyton.2015.84.128

    Abstract Losses due to postharvest decay may occur at any time during postharvest handling, from harvest to consumption affecting the produce quality and quantity. Accurate identification of the pathogen causing postharvest disease is essential to the selection of an appropriate disease control approach. Nine isolates of Fusarium recovered from orange fruit were identified as Fusarium solani. The fungus is involved with fruit decay. The obtained cultures were purified and grown on potato-dextrose agar (PDA), malt yeast agar (MYA), and Czapek's nutrient media (CNM) under light for identification. A pathogenicity test was carried out to fulfil Koch's postulates. More >

  • Open Access

    ARTICLE

    Growth rate and pathogenicity of isolates of Diaporthe phaseolorum var. caulivora

    Grijalba P1 y A del C Ridao2

    Phyton-International Journal of Experimental Botany, Vol.83, pp. 325-332, 2014, DOI:10.32604/phyton.2014.83.325

    Abstract Diaporthe phaseolorum var. caulivora is the major causal agent of stem canker in soybean (CTS) in Argentina. It has appeared mainly in the Southern Pampeana sub-region, but also in warm areas, and it has been observed with different levels of incidence, severity and virulence. The objective of this work was to study the growth rate in vitro and the pathogenicity of isolates from soybean plants at different temperatures. Twenty isolates from Buenos Aires Province were selected. The daily growth rate was measured in nine of them and in two reference isolates in potato dextrose agar (APD), under… More >

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