Ziliang Zhou*†1, Lijie Zhou*‡1, Fangfang Jiang*, Binghui Zeng*, Changbo Wei*, Wei Zhao*, Dongsheng Yu*
Oncology Research, Vol.25, No.2, pp. 207-214, 2017, DOI:10.3727/096504016X14732772150460
Abstract Previous studies have shown that miR-222 targets the p53 upregulated modulator of apoptosis (PUMA) to
regulate cell biological behavior in some human malignancies. We hypothesized that there was a negative
regulation, which might induce apoptosis, between miR-222 and PUMA in adenoid cystic carcinoma (ACC).
In this study, the expression levels of miR-222 and the PUMA gene after transfection with antisense miR-222
(As-miR-222) were evaluated by RT-PCR and Western blot assays. Cell proliferation and migratory abilities
were detected by CCK-8 and Transwell assays. Cell cycle and apoptosis were analyzed by flow cytometry. Our
results showed that, More >
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