Qian Tao, Guiqi Han, Bujin Ma, Hongmei Jia, Can Zhao, Wenshang Li, Zhuyun Yan^*

Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1297-1310, 2024, DOI:10.32604/phyton.2024.051923

Abstract Fritillaria cirrhosa D. Don (Liliaceae) is an endangered perennial bulbous plant and its dry bulb is a valuable medicinal material with antitussive and expectorant effects. Nevertheless, lack of resources and expensive prices make it difficult to meet clinical needs. This study presents a regeneration system aimed at overcoming the challenge of inadequate supply in F. cirrhosa, focusing on: (1) callus induction, (2) bulblets and adventitious bud induction, and (3) artificial seed production. Callus development was achieved in 84.93% on Murashige and Skoog (MS) medium fortified with 1.0 mg·L picloram. The optimal medium for callus differentiation into regenerated… More >

Mir Abdullatif Yahya¹, Fatma Tunali¹, Dilek Killi^2,*, Atalay Sökmen²

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 427-444, 2024, DOI:10.32604/phyton.2024.046271

Abstract An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller, a medicinal aromatic plant that is a prominent source of volatile organic compounds (VOCs). Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators (PGRs), and the growth parameters of the nodal segments were examined. Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine (BAP). The superior hormonal concentration of Murashige and Skoog (MS) media for the proliferation of shoots from callus cultures (39.33%) was 5 mg/L of 2-Isopentenyl adenine (2iP), and the favorable media for the growth… More >

Xuan Sun^1,2, Xue Wang¹, Bijaya Sharma Subedi³, Yin Jiang^1,2, Di Wang^1,2, Rongxin Gou^1,2, Guojun Zhang², Wenting Xu^4,*, Zunzheng Wei^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.8, pp. 2413-2428, 2023, DOI:10.32604/phyton.2023.029667

Abstract The calla lily (Zantedeschia spreng.) is a bulbous flower native to the tropical regions of Africa. Calla lily has gained significant popularity in the international market owing to its intricate morphology and prolonged flowering duration. Despite such advantages, for two sub-groups of calla lily, known as group Zantedeschia and group Aestivae, there are challenges in terms of hybrid production due to the ‘plastome-genome incompatibility’ therebetween. Tissue culture is a fundamental biotechnological tool used in gene editing research, with a focus on disease resistance and flower color in calla lily breeding programs. The present review provides a brief… More >

Zongmin Mou^1,2,3, Fei Ye⁴, Fangchen Shen¹, Dake Zhao^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2331-2348, 2022, DOI:10.32604/phyton.2022.021273

Abstract Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation and environment disturbance. This study was conducted to provide propagation methods through in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L⁻¹ 6-benzylaminopurine (BAP), 0.1 mg L⁻¹ 1-napthaleneacetic acid (NAA), and 30 g L⁻¹ sucrose. Three bacteria (Pantoea agglomerans, Erwinia persicina, and Pseudomonas tolaasii) would be responsible for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the… More >

Jiaji Zhang¹, Xiaofei Long¹, Yuhao Weng¹, Tielong Cheng², Jisen Shi¹, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 571-582, 2021, DOI:10.32604/phyton.2021.014191

Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N⁶ -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA₃). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L^–1 BA, 0.05 mg L^–1 NAA and… More >

MARÍA LAURA FONTANA, LUIS AMADO MROGINSKI, HEBE YOLANDA REY*

BIOCELL, Vol.33, No.3, pp. 179-186, 2009, DOI:10.32604/biocell.2009.33.179

Abstract With the aim of developing an efficient plant regeneration protocol, leaflet explants of three accessions of Arachis villosa Benth. (S2866, S2867 and L97) were cultured on basic Murashige and Skoog medium supplemented with different combinations of plant growth regulators: α-naphthalenacetic acid, indole-3-butyric acid, 6-benzylaminopurine, kinetin and thidiazuron. The accession L97 was the only one able to differentiate buds through indirect organogenesis. The most suitable combination for bud regeneration was the basic medium added with 13.62 μM thidiazuron and 4.44 μM 6-benzylaminopurine. These results show the important role of the genotype in morphogenetic responses and the organogenetic effect of More >

E. KOCH¹, B.C. WINIK², A. CASTRO-VAZQUEZ^1,3,*

BIOCELL, Vol.33, No.1, pp. 49-65, 2009, DOI:10.32604/biocell.2009.33.049

Abstract Development of Pomacea canaliculata from the gastrula stage until the first day after hatching is described. Trochophore embryos are developed after gastrulation, showing the prototroch as a crown of ciliated orange-brownish cells. However, no true veliger embryos are formed, since the prototroch does not fully develop into a velum. Afterward, the connection between the fore- and midgut is permeated and the midgut becomes full of the pink-reddish albumen, which is stored into a central archenteron’s lake, from where it is accumulated into the large cells forming the midgut wall (“giant cells”). Electron microscopy of giant cells… More >

S.K. VILA*, H.Y. REY, L.A. MROGINSKI

BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l^-1 benzylaminopurine (BAP) + 0.1mg.l^-1 kinetin (KIN) + 3 mg.l^-1 adenine sulphate (ADS).
The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the More >

Li RS¹, YJ Tao², FJ Liu², X Hu², QL Xu², KY Li²

Phyton-International Journal of Experimental Botany, Vol.85, pp. 87-93, 2016, DOI:10.32604/phyton.2016.85.087

Abstract The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants (axillary… More >

Guan Z-J^1,3,4*, S-B Lu^2*,Y-L Huo⁵, B Guo³, Z-P Guan⁶, Y-H Wei³

Phyton-International Journal of Experimental Botany, Vol.84, No.2, pp. 351-357, 2015, DOI:10.32604/phyton.2015.84.351

Abstract Leaf explants of the HBsAg-transgenic cherry tomato (Solanum lycopersicum) mutant were cultured on Murashige and Skoog (MS) basal medium, supplemented with 1.0 mg/L 6-BA and 0.05 mg/L IAA for callus induction, to clarify the physiological and biochemical characteristics of morphogenesis development. Therefore, the physiological and biochemical changes during the development of organogenic shoots and somatic embryos in the mutant were studied. Superoxide dismutase (SOD) activities of the mutant had only one peak value on the 21st day. Peroxidase (POD) activities of the mutant declined less sharply since the explants were cultured. IAA oxidase activity of… More >