Lu Zhu, Qiuyue Ma, Shushun Li, Jing Wen, Kunyuan Yan, Qianzhong Li*
Phyton-International Journal of Experimental Botany, Vol.89, No.2, pp. 385-403, 2020, DOI:10.32604/phyton.2020.09259
- 22 April 2020
Abstract Quantitative real-time reverse transcriptase PCR (qRT-PCR) technology has been extensively used to estimate gene expression levels, and the selection of appropriate reference genes for qRT-PCR analysis is critically important
for obtaining authentic normalized data. Acer palmatum is an important colorful
leaf ornamental tree species, and reference genes suitable for normalization of the
qRT-PCR data obtained from this species have not been investigated. In this
study, the expression stability of ten candidate reference genes, namely, Actin3,
Actin6, Actin9, EF1α, PP2A, SAMDC, TIP41, TUBα, TUBβ and UBQ10, in
two distinct tissues (leaves and roots) of A. palmatum under four different abiotic
stress conditions (cold,… More >
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