Yan Li^*, Shan Ji^†, Li-Ye Fu^*, Tao Jiang^*, Di Wu^*, Fan-Dong Meng^*

Oncology Research, Vol.25, No.5, pp. 721-731, 2017, DOI:10.3727/096504016X14772375848616

Abstract Cyclin-dependent kinase inhibitor 3 (CDKN3) has been reported to promote tumorigenesis. Since it is unclear whether CDKN3 participates in the development of human gastric cancer, this study assessed the association between CDKN3 expression and cell biological function and demonstrated the clinical significance and prognosis of CDKN3 in human gastric cancer. In this study, we found that CDKN3 showed a high expression in 35 paired human gastric cancer tissues and was correlated with poor patient survival, AJCC clinical staging, and recurrence. Silencing of CDKN3 in human gastric cancer cells can significantly reduce proliferation, migration, invasion, and More >

Feng Shuai^*, Bo Wang^†, Shuxiao Dong^‡

Oncology Research, Vol.26, No.8, pp. 1295-1305, 2018, DOI:10.3727/096504018X15172747209020

Abstract Among all of the miRNAs, miR-204 has gained considerable attention in the field of cancer research. This study aimed to reveal the detailed functions and the underlying mechanism of miR-204 in colorectal cancer (CRC) cells. The expressions of miR-204 in CRC tumor tissues and cell lines were monitored. Expressions of miR-204 and CXCL8 in Caco-2 and HT-29 cells were altered by transfection, and then cell viability, apoptosis, migration, invasion, EMT-related protein expression, and PI3K/AKT/mTOR pathway protein expression were assessed. We found that miR-204 was expressed at low levels in CRC tumor tissues and cell lines… More >

I Wayan Sumardika^*†, Chen Youyi^*, Eisaku Kondo^‡, Yusuke Inoue^§, I Made Winarsa Ruma^*†, Hitoshi Murata^*, Rie Kinoshita^*, Ken-Ichi Yamamoto^*, Shuta Tomida^¶, Kazuhiko Shien^#, Hiroki Sato^#, Akira Yamauchi^**, Junichiro Futami^††, Endy Widya Putranto^‡‡, Toshihiko Hibino^§§, Shinichi Toyooka^¶#¶¶, Masahiro Nishibori^##, Masakiyo Sakaguchi^*

Oncology Research, Vol.26, No.3, pp. 431-444, 2018, DOI:10.3727/096504017X15031557924123

Abstract We previously identified novel S100A8/A9 receptors, extracellular matrix metalloproteinase inducer (EMMPRIN), melanoma cell adhesion molecule (MCAM), activated leukocyte cell adhesion molecule (ALCAM), and neuroplastin (NPTN) β, that are critically involved in S100A8/A9-mediated cancer metastasis and inflammation when expressed at high levels. However, little is known about the presence of any cancerspecific mechanism(s) that modifies these receptors, further inducing upregulation at protein levels without any transcriptional regulation. Expression levels of glycosyltransferase-encoding genes were examined by a PCRbased profiling array followed by confirmation with quantitative real-time PCR. Cell migration and invasion were assessed using a Boyden chamber.… More >

TAO LIU¹, LIMIN ZHOU¹, LIANBO ZHANG², XIN GUAN³, YI DONG^1,*

Oncology Research, Vol.29, No.3, pp. 189-200, 2021, DOI:10.32604/or.2022.03573

Abstract Many studies have illustrated the significance of long noncoding RNAs in oncogenesis and promotion of breast cancer (BC). However, the biological roles of CCDC183 antisense RNA 1 (CCDC183-AS1) in BC have rarely been characterized. Thus, we explored whether CCDC183-AS1 is involved in the malignancy of BC and elucidated the possible underlying mechanisms. Our data confirmed elevated CCDC183-AS1 expression in BC, which was associated with poor clinical outcomes. Functionally, knocking down CCDC183-AS1 hampered cell proliferation, colony formation, migration, and invasion in BC. Additionally, the absence of CCDC183-AS1 restrained tumor growth in vivo. Mechanistically, CCDC183-AS1 executed as a More >

Feng Shuai^*, Bo Wang^†, Shuxiao Dong^‡

Oncology Research, Vol.28, No.9, pp. 975-975, 2020, DOI:10.3727/096504021X16297182567090

Abstract This article has no abstract. More >

Çağrı Öner^1,* and Ertuğrul Çolak²

Oncologie, Vol.23, No.3, pp. 393-407, 2021, DOI:10.32604/Oncologie.2021.016958

Abstract piRNAs are novel members of small non-coding RNAs and have an impact on genetic and epigenetic mechanisms of cells. It was aimed to investigate the role of piR-651 on MCF-7 benign breast cancer cells by focusing on molecular characteristics. Anti-piR-651 was transfected and effects of piR-651 on proliferation, adhesion, and motility of MCF-7 cells were detected after the 24th, 48th, and 72nd hour. Gene expressions of piR-651, Ki-67, MMP-2, ERα, HIF-1α, and hTERT were determined by using RT-PCR. piR-651 inhibition caused the decrease of proliferation, adhesion (p < 0.001), and motility of MCF-7 cells. The efficiency… More >

TIECHENG SUN^1,#, YANDONG ZHANG^1,#, JIANHUA LI^3,#, HONG YU¹, LINGLI SONG¹, XINPING SUN¹, XI CHEN^2,*, LI TIAN^1,2,*

BIOCELL, Vol.45, No.3, pp. 705-710, 2021, DOI:10.32604/biocell.2021.014694

Abstract This study aims to investigate the protective effects of peroxiredoxin 6 on the total motility and progressive motility of human spermatozoa. Semen samples with normal parameters were collected from 23 males and supplemented with different concentrations of peroxiredoxin 6. All the semen samples were measured according to the WHO 5th manual, and the motile spermatozoa were extracted using IVF fertilization medium supplemented with different peroxiredoxin 6 concentrations. Total motility and progressive motility were observed at different timepoints of culture at room temperature. After peroxiredoxin 6 supplementation, all groups had a significant increase in total motility… More >

TIECHENG SUN^1,#, LUYANG CHENG^2,#, JING MA^3,#, SHANJIE ZHOU¹, YANDONG ZHANG¹, WENDI ZHOU⁴, SHOULONG DENG⁵, SHUSONG WANG^3,*, TIAN LI^1,6,*

BIOCELL, Vol.44, No.3, pp. 323-327, 2020, DOI:10.32604/biocell.2020.011890

Abstract Sperm cryopreservation is useful in assisted reproductive technology and male fertility preservation. However, freezing and thawing significantly reduces the total and progressive motility of human spermatozoa. In the present study, we explored the effects of peroxiredoxin 6 (PRDX6) on total and progressive motility of human spermatozoa after cryopreservation. Semen samples of 20 males with normal parameters were collected and frozen in media supplemented with different concentrations of PRDX6 (0 mM, 10⁻⁵ mM, 10⁻⁷ mM, and 10⁻⁹ mM, respectively). Postthaw total and progressive motility of sperms were measured. The results showed that in comparison with 0 mM, the More >

RANDI ZUKAS¹, ALEX J. CHANG¹, MARIAN RICE², AMY L. SPRINGER^1*

BIOCELL, Vol.36, No.3, pp. 133-142, 2012, DOI:10.32604/biocell.2012.36.133

Abstract Trypanosoma brucei is a protozoan flagellate that causes African sleeping sickness. Flagellar function in this organism is critical for life cycle progression and pathogenesis, however the regulation of flagellar motility is not well understood. The flagellar axoneme produces a complex beat through the precisely coordinated firing of many proteins, including multiple dynein motors. These motors are found in the inner arm and outer arm complexes. We are studying one of the inner arm dynein motors in the T. brucei flagellum: dynein-f. RNAi knockdown of genes for two components of dynein-f: DNAH10, the α heavy chain,… More >

ROBERTO YUNES, PEDRO FERNÁNDEZ, GUSTAVO F. DONCEL, ANÍBAL A. ACOSTA

BIOCELL, Vol.29, No.3, pp. 287-293, 2005, DOI:10.32604/biocell.2005.29.287

Abstract Our objective was to determine the effect of phosphodiesterase (PDE) inhibition on: 1) tyrosine phosphorylation of human spermatozoa at the tail level; and 2) sperm motion parameters and hyperactivated motility. The study was conducted with normozoospermic and asthenozoospermic samples incubated under in vitro capacitating conditions. The main outcome measures were computer-assisted sperm motion analysis and fluorescent immunodetection of phosphotyrosine-containing proteins. Pentoxifylline (PTX) was used as PDE inhibitor because of its wide use in the clinic. PTX-treatment significantly increased sperm velocity, hyperactivated motility and tyrosine-phosphorylation, both in normo and asthenozoospermic samples. Tyrosine-phosphorylation of tail proteins was highly More >