WEIYU CHEN^1,2, MUHSIN H. YOUNIS³, ZHONGKUO ZHAO^1,*, WEIBO CAI^3,*

BIOCELL, Vol.46, No.8, pp. 1789-1801, 2022, DOI:10.32604/biocell.2022.018197

Abstract The knowledge of interactions among functional proteins helps researchers understand disease mechanisms and design potential strategies for treatment. As a general approach, the fluorescent and affinity tags were employed for exploring this field by labeling the Protein of Interest (POI). However, the autofluorescence and weak binding strength significantly reduce the accuracy and specificity of these tags. Conversely, HaloTag, a novel self-labeling enzyme (SLE) tag, could quickly form a covalent bond with its ligand, enabling fast and specific labeling of POI. These desirable features greatly increase the accuracy and specificity, making the HaloTag a valuable system for various applications ranging from… More >

Farnaz Nabiya¹, Anchana Devi Chenniappan^1,*, Rajamiriyam Marichamy¹, MubarakAli Davoodbasha^2,3, Jung-Wan Kim^3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 569-582, 2022, DOI:10.32604/phyton.2022.017390

Abstract Endometriosis is a chronic inflammatory disease that occurs due to the presence of endometrial tissue outside the uterine cavity. It affects from 5% to 10% of women of reproductive age. High levels of matrix metalloproteinase (especially MMP-9) have been observed in women suffering from endometriosis. Thus, the aim of this study was to investigate the naturally anti-inflammatory compounds available from an algal source that can target the MMP-9 by various in silico approaches. The target 1L6J (Crystal structure of human matrix metalloproteinase MMP-9) structure was retrieved from the PDB database. Five compounds such as Eckol, Sargafuran, Vitamin E, Docosahexaenoic acid,… More >

PENGFEI ZHANG¹, SHAOPENG WANG^1,2,*

BIOCELL, Vol.45, No.6, pp. 1449-1451, 2021, DOI:10.32604/biocell.2021.017607

Abstract The exosome-mediated response can promote or restrain the diseases by regulating the intracellular pathways, making the exosome become an effective marker for diagnosis and therapeutic control at the single-cell level. However, real-time analysis is hard to be achieved with traditional approaches because the exosomes usually need to be enriched by ultracentrifugation for a measurable signal-to-noise ratio. Recently developed label-free single-molecule imaging approaches may become an real-time quantitative tool for the analysis of single exosomes and related secretion behaviors of single living cells owing to their extreme sensitivity. More >

Dan Tian^1,*, Danni Yu², Chunhui He³

FDMP-Fluid Dynamics & Materials Processing, Vol.17, No.4, pp. 711-720, 2021, DOI:10.32604/fdmp.2021.014290

Abstract In the search for sustainable alternatives to harmful synthetic fibers, an increasing amount of research focuses on biomimicry and natural fibers. Sea silk is an exceptional textile material. It is a kind of natural silk produced using the long silky filaments secreted by a specific bivalve mollusk (Pinna nobilis); now at edge of extinction. This paper suggests a simple but effective way to prepare artificial sea silk from Mytilus edulis. A sea silk solution is prepared using a Mytilus edulis protein, and a polyvinyl alcohol (PVA) solution is mixed with the sea silk solution in order to produce artificial sea… More >

LITING YOU^1,#, FEIFEI NA^2,#, JUAN ZHOU³, LIN JIAO³, YI ZHOU^3,*, BINWU YING^3,*

BIOCELL, Vol.45, No.3, pp. 649-663, 2021, DOI:10.32604/biocell.2021.013978

Abstract Reviews The Dectin-1 cluster comprises seven members: CLEC-12A, CLEC-12B, CLEC-1A, CLEC-7A, CLEC- 2, CLEC-9A and OLR1. These members have been demonstrated to be involved in the tumorigenesis, progression, and metastasis of several cancers. However, little is known about their roles in human lung adenocarcinoma (LUAD). The expression patterns of the Dectin-1 cluster were analyzed via the ONCOMINE and GEPIA databases. We evaluated the prognostic value of the Dectin-1 cluster in patients with LUAD using the Kaplan-Meier plotter and GEPIA. Differential expression was validated with the EMBL-EBI database, and protein expression was analyzed with the HPA database. In addition, protein-protein interaction… More >

CHEN CAI¹, HAO SUN², LIANG HU³, ZHICHAO FAN^1,*

BIOCELL, Vol.45, No.2, pp. 229-257, 2021, DOI:10.32604/biocell.2021.014338

Abstract Integrin molecules are transmembrane αβ heterodimers involved in cell adhesion, trafficking, and signaling. Upon activation, integrins undergo dynamic conformational changes that regulate their affinity to ligands. The physiological functions and activation mechanisms of integrins have been heavily discussed in previous studies and reviews, but the fluorescence imaging techniques –which are powerful tools for biological studies– have not. Here we review the fluorescence labeling methods, imaging techniques, as well as Förster resonance energy transfer assays used to study integrin expression, localization, activation, and functions. More >

Resa Martha^1,2, Mahdi Mubarok^1,2, Wayan Darmawan², Wasrin Syafii², Stéphane Dumarcay¹, Christine Gérardin Charbonnier¹, Philippe Gérardin^1,*

Journal of Renewable Materials, Vol.9, No.3, pp. 399-449, 2021, DOI:10.32604/jrm.2021.014286

Abstract As a tropical archipelagic country, Indonesia’s forests possess high biodiversity, including its wide variety of wood species. Valorisation of biomolecules released from woody plant extracts has been gaining attractive interests since in the middle of 20th century. This paper focuses on a literature review of the potential valorisation of biomolecules released from twenty wood species exploited in Indonesia. It has revealed that depending on the natural origin of the wood species studied and harmonized with the ethnobotanical and ethnomedicinal knowledge, the extractives derived from the woody plants have given valuable heritages in the fields of medicines and pharmacology. The families… More >

MASOUMEH OGHABI^1,2, AVA SAFAROGHLI-AZAR^1,2, ATIEH POURBAGHERI-SIGAROODI^1,2, MOHAMMAD SAYYADI³, MOHSEN HAMIDPOUR¹, MOHAMMAD HOSSEIN MOHAMMADI¹, DAVOOD BASHASH^1,*

BIOCELL, Vol.44, No.2, pp. 183-192, 2020, DOI:10.32604/biocell.2020.08880

Abstract Pathogenesis of chronic myeloid leukemia (CML) has mostly been studied with regard to the oncogenic role of BCR/ABL fusion; however, recent disclosures have declared that the challenges with the treatment of CML patients would not be resolved until the role of other aberrancies is ignored. Given the involvement of cyclin-dependent kinases (CDKs) in the pathogenesis of CML, the present study aimed to investigate the effects of a multi-CDK inhibitor AT7519 on BCR/ABL-harboring CML-derived K562 cells. Our results showed that AT7519 effectively reduced the survival of K562 and induced its anti-proliferative effect through the induction of G2/M arrest due to elevated… More >

GRACIELA CRISTINA CALABRESE*, ROSA WAINSTOK**

BIOCELL, Vol.28, No.3, pp. 251-258, 2004, DOI:10.32604/biocell.2004.28.251

Abstract Endothelial cells, at the cell-cell borders, express PECAM-1, and have been implicated in vascular functions. The monoclonal antibody MEC 13.3 recognizes PECAM-1 molecule from mouse vessels and allows to analyze the ontogeny of mouse endothelium. At the present, little is known about the molecular basis of differentiation pathways of endothelial cells, that enables its morphological heterogeneity. The purpose of this study was to analyze the pattern of PECAM-1 expression, employing monoclonal antibody MEC 13.3, in cellular suspensions obtained from different mouse organs at pre and postnatal stages.
Fluorescence activated cell sorter analysis showed a different profile of the glycoprotein… More >

W. Chen¹, L. Wang¹

Molecular & Cellular Biomechanics, Vol.3, No.4, pp. 203-204, 2006, DOI:10.32604/mcb.2006.003.203

Abstract This article has no abstract. More >