A. A. Román-Calzoncit¹, F. Ramírez-Godina^2,*, J. Sánchez-Laureano², A. C. Flores-Gallegos¹, J. Espinoza-Velázquez², R. Rodríguez-Herrera^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 87-97, 2021, DOI:10.32604/phyton.2020.013880 - 20 November 2020

Abstract Polyembryony in maize (PEm) contributes to improving the nutritional properties of the grain, as well as an increase in yield, since it generates multiple plants per seed, opening the possibility of developing new varieties. However, it is unknown whether polyembryony in maize is the product of chromosomal abnormalities. Based on the above, in this research a cytogenetic study was proposed to verify if chromosomal abnormalities are related to the maize polyembryony. For a meiotic study, maize genotypes with variable proportions of polyembryony (PEm), from the UA-IMM-BAP population and non-PEm (monoembryonic) maize were used, while for… More >

Qin Peng¹, Shaoying Lu¹, Shu Chien¹, Yingxiao Wang^1,*

Molecular & Cellular Biomechanics, Vol.16, Suppl.2, pp. 83-83, 2019, DOI:10.32604/mcb.2019.07385

Abstract The dramatic re-organization of chromatin during mitosis is perhaps one of the most fundamental of all cell processes [1,2]. It remains unclear how epigenetic histone modifications, despite their crucial roles in regulating chromatin architectures, are dynamically coordinated with chromatin reorganization in controlling this process. Mechanical cues have also been shown to play important roles in modulating gene expressions and cellular functions [3,4]; however, it is still unclear about the mechanical regulations of epigenetics and chromatin organization. In this study, we have developed and characterized biosensors with high sensitivity and specificity based on fluorescence resonance energy… More >

Hernández-Quintero JD¹, MH Reyes-Valdés², DV Mendoza-Rodríguez³, M Gómez-Martínez², R Rodríguez-Herrera⁴

Phyton-International Journal of Experimental Botany, Vol.84, No.1, pp. 107-112, 2015, DOI:10.32604/phyton.2015.84.107

Abstract The genus Dasylirion is a group of plants typically present in the Chihuahuan Desert, perennial, with a dioecious sexual behavior and commonly called sotoles. This genus has been little studied from the biological point of view, and the bases of its reproductive response remain unknown. In this work we studied the chromosome number and meiotic response of Dasylirion cedrosanum in the county of Saltillo, Coahuila, located at the North East of Mexico. For the preparation of mitotic chromosomes, we used a technique based on enzymatic treatment with pectolyase and cellulase, as well as staining with acetocarmin… More >

ANNIE JOUBERT^1*, SUMARI MARAIS¹, CHRISTINE MARITZ²

BIOCELL, Vol.33, No.1, pp. 67-70, 2009, DOI:10.32604/biocell.2009.33.067

Abstract Proteins of the B-cell lymphoma 2 family are crucial for the regulation of apoptosis. B-cell lymphoma 2-associated X is a pro-apoptotic protein, while B-cell lymphoma 2 protein opposes apoptosis. The influence of 1 μM 2-methoxyestradiol was investigated on the expression levels of these two proteins in MCF-7 cells. 2-Methoxyestradiol exposure did not influence B-cell lymphoma 2 protein expression levels after 24 h of exposure. In contrast, B-cell lymphoma 2-associated X protein levels were significantly reduced. An improved differential interference contrasting technique revealed compromised cell density and the presence of a mitotic block in exposed cells. More >

DONGHUA LIU1*, WUSHENG JIANG², QINGMIN MENG¹, JIN ZOU¹, JIEGANG GU¹, MUAI ZENG¹

BIOCELL, Vol.33, No.1, pp. 25-32, 2009, DOI:10.32604/biocell.2009.33.025

Abstract Different copper concentrations, as well as different exposure times, were applied to investigate both cytogenetical and ultrastructural alterations in garlic (Allium sativum L.) meristem cells. Results showed that the mitotic index decreased progressively when either copper concentration or exposure time increased. C-mitosis, anaphase bridges, chromosome stickiness and broken nuclei were observed in the copper treated root tip cells. Some particulates containing the argyrophilic NOR-associated proteins were distributed in the nucleus of the root-tip cells and the amount of this particulate material progressively increased with increasing exposure time. Finally, the nucleolar material was extruded from the nucleus More >

ALBERTO J. SOLARI*, MONICA I. RAHN*, ALICIA SAURA**, HUGO D. LUJAN**

BIOCELL, Vol.27, No.3, pp. 329-346, 2003, DOI:10.32604/biocell.2003.27.329

Abstract The fine structure of the binucleate, parasitic protist Giardia lamblia during interphase and divisional stages was studied by serial thin sectioning and three-dimensional reconstructions. The earlier sign of nuclear division is the development of a few peripheral areas of densely packed chromatin directly attached to the inner nuclear envelope. An intracytoplasmic sheet of ventral disk components grows from the cell periphery towards one of the nuclei, apparently constricting this nucleus, which becomes located at a ventral bulge. After the basal bodies become duplicated, a full nuclear division occurs in trophozoites, giving two pairs of parent-daughter nuclei.… More >