Mir Abdullatif Yahya¹, Fatma Tunali¹, Dilek Killi^2,*, Atalay Sökmen²

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 427-444, 2024, DOI:10.32604/phyton.2024.046271 - 28 March 2024

Abstract An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller, a medicinal aromatic plant that is a prominent source of volatile organic compounds (VOCs). Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators (PGRs), and the growth parameters of the nodal segments were examined. Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine (BAP). The superior hormonal concentration of Murashige and Skoog (MS) media for the proliferation of shoots from callus cultures (39.33%) was 5 mg/L of 2-Isopentenyl adenine (2iP), and the favorable media for the growth… More >

Shijie Lin¹, Zimo Wang¹, Hongbo Zhu², Conghui Wang³, Hongfeng Wang², Dawei Zhang¹, Tianbing Gou¹, Guangdao Bao¹, Ye Luo¹, Huaijiang He¹, Zhonghui Zhang^1,*

Phyton-International Journal of Experimental Botany, Vol.93, No.2, pp. 277-289, 2024, DOI:10.32604/phyton.2024.046989 - 27 February 2024

Abstract Tilia amurensis is an economically valuable broadleaf tree species in Northeast China. The production of high-quality T. amurensis varieties at commercial scales has been greatly limited by the low germination rates. There is thus a pressing need to develop an organogenesis protocol for in vitro propagation of T. amurensis to alleviate a shortage of high-quality T. amurensis seedlings. Here, we established a rapid in vitro propagation system for T. amurensis from mature zygotic embryos and analyzed the effects of plant growth regulators and culture media in different stages. We found that Woody plant medium (WPM) was the optimal primary culture medium for… More >

Esraa E. Shalan¹, Said S. Soliman¹, Ahmed A. Mahmoud¹, Jameel M. Al-Khayri^2,*, Salha M. ALshamrani³, Fatmah A. Safhi⁴, Areej S. Jalal⁴, Diaa Abd El-Moneim⁵, Abdallah A. Hassanin^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2183-2196, 2023, DOI:10.32604/phyton.2023.028537 - 29 May 2023

Abstract Determination of the somaclonal variation of in vitro-propagated plants is crucial to determine the appropriate micropropagation protocol and growth regulators for commercial scale multiplication. In this research, nine multiplication media (MM) augmented with different concentrations of 6-benzyl adenine (BA), Kinetin (Kin), and Thidiazuron (TDZ), Three rooting media (RM) supplemented with three levels of α-naphthalene acetic acid (NAA) and three types of soil mixtures (v/v); Coco peat/Vermiculite/Sand (CVS), Peat moss/Perlite/Sand (PPS) and Peat moss/Perlite (PP) were used in the micropropagation protocol of daylily plants. MM2 showed the maximum shoot length and the number of leaves, while MM9… More >

Zongmin Mou^1,2,3, Fei Ye⁴, Fangchen Shen¹, Dake Zhao^1,2,3,*

Phyton-International Journal of Experimental Botany, Vol.91, No.10, pp. 2331-2348, 2022, DOI:10.32604/phyton.2022.021273 - 30 May 2022

Abstract Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation and environment disturbance. This study was conducted to provide propagation methods through in vitro germination and explant cultivation. Germination was stimulated up to 66.00% on Murashige and Skoog (MS) medium containing 2.0 mg L⁻¹ 6-benzylaminopurine (BAP), 0.1 mg L⁻¹ 1-napthaleneacetic acid (NAA), and 30 g L⁻¹ sucrose. Three bacteria (Pantoea agglomerans, Erwinia persicina, and Pseudomonas tolaasii) would be responsible for consistent contamination during germination. The latter two were effectively eradicated after disinfected. The influence of explant types and hormone combinations on direct and indirect organogenesis was evaluated in the… More >

Lucía Isabel Chávez-Ortiz¹, José Francisco Morales-Domínguez¹, Araceli Rodríguez-Sahagún², Eugenio Pérez-Molphe-Balch^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.3, pp. 1003-1013, 2021, DOI:10.32604/phyton.2021.012862 - 30 March 2021

Abstract Agave guiengolaGentry is an endemic plant from a very small locality in Oaxaca, Mexico. Its conservation status is fragile and can rapidly worsen. Because of its scarcity, this agave has been used solely for ornamental purposes, but it could have other uses if more plants were available. In vitro propagation by enhanced axillary sprouting from stem segments was attained using Murashige and Skoog Basal Medium (MS) as well as basal medium supplemented with cytokinins 6-Benzylaminopurine (BA) or 6-(γ,γ-Dimethylallylamino)purine (2iP). The best treatment for shoot induction in semisolid medium consisted in MS supplemented with 2 mg l^–1 BA, obtaining… More >

Jiaji Zhang¹, Xiaofei Long¹, Yuhao Weng¹, Tielong Cheng², Jisen Shi¹, Jinhui Chen^1,*

Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 571-582, 2021, DOI:10.32604/phyton.2021.014191 - 07 February 2021

Abstract Cinnamomum japonicum Sieb. is an excellent roadside tree and medicinal tree species with considerable ornamental and economic value. In this study, we successfully developed a large-scale micropropagation protocol for C. japonicum for the first time. Sterilized shoots were excised and used as explants for shoot induction on several basal media, supplemented with different concentrations of plant growth regulators (PGRs), such as Thidiazuron (TDZ), N⁶ -Benzyladenine (6-benzylaminopurine) (BA), α-naphthaleneacetic acid (NAA) and Gibberellic acid (GA₃). After comparison, the most efficient medium for shoot regeneration was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg L^–1 BA, 0.05 mg L^–1 NAA and… More >

Krupa-Małkiewicz M¹, B Smolik², M Sędzik²

Phyton-International Journal of Experimental Botany, Vol.88, No.1, pp. 15-23, 2019, DOI:10.32604/phyton.2019.04670

Abstract Salinity is one of the abiotic stresses that limits the growth and productivity of many crops. A possible survival strategy for plant under saline conditions is to use compounds that could minimize the harmful effects of salt stress on the plant development. The objective of the presented study was to investigate the effect of exogenous ascorbic acid (ASA) with or without gibberellic acid (GA3) on key growth and biochemical parameters in two petunia cultivars ‘Prism Rose’ and ‘Prism White’ under saline (150 mM NaCl) and non-saline in vitro condition. Nodal cutting with an axillary buds… More >

Jimarez-Montiel MJ¹, A Robledo-Paz¹, VM Ordaz-Chaparro², LI Trejo-Tellez², JC Molina-Moreno¹

Phyton-International Journal of Experimental Botany, Vol.87, pp. 94-104, 2018, DOI:10.32604/phyton.2018.87.094

Abstract An alternative method for improving the production of habanero chili is the tissue culture technique; however, the gelling agent, the high salt and sucrose concentrations used in the culture media raise production costs and limit the adaptation of the regenerated plants to greenhouse or field conditions. In this study, the effect of the substrates perlite-coconut fiber, coconut fiber-volcanic rock, vermiculite-perlite, and perlite-volcanic rock in conjunction with various culture media in in vitro plant regeneration from embryos was evaluated. The differentiation of adventitious shoots on substrates was scarcely observed or non-existent. Inducing the formation of shoots on… More >

Jiménez-Martínez JH¹, MG Gutiérrez-Martínez², O Franco-Mora¹, A González-Huerta², AT Gutiérrez-Ibáñez²

Phyton-International Journal of Experimental Botany, Vol.82, pp. 107-112, 2013, DOI:10.32604/phyton.2013.82.107

Abstract Two accessions of wild grapevines (Vitis spp.) native to Puebla State, Nealticán and Teziutlán, and other two native to Temascaltepec (1 and 2), Mexico State, were cultured in vitro using both Murashige-Skoog (MS) and woody plant medium (WPM) mediums. There were no differences between mediums for shoot generation. However, differences were shown among accessions for explant vigour, callus development, number of roots, leaves and nodes per explant. Rooting was evaluated only in MS medium by adding naphthalene acetic acid (NAA), indole butyric acid (IBA) or indole acetic acid (IAA) at 0.5 mg/L. With the exception of Temascaltepec… More >

Sadia^1,3 B, PC Josekutty², SD Potlakayala², P Patel², S Goldman³, SV Rudrabhatla²

Phyton-International Journal of Experimental Botany, Vol.79, pp. 177-181, 2010, DOI:10.32604/phyton.2010.79.177

Abstract A robust protocol for culturing meristems of Sorghum is required to assist with rapid genetic improvement of the genus. Through meristem culture, an efficient method for rapid micropropagation was developed for Sorghum bicolor (L.) Moench. hybrids, namely NC+262, NC+6C21 and NC+6B50. Complete plants were regenerated directly from shoot meristems without an intervening callus phase. Regeneration frequencies varied between the studied genotypes and according to the growth regulator combinations present in the medium. The combination of BAP and TDZ showed a synergistic effect on shoot multiplication. The highest number of shoots per meristem (68 ± 2) was recorded More >