Tao Liang, Xiao-Yong Hu, Yong-Hui Li, Bin-Qiang Tian, Zuo-Wei Li, Qiang Fu

Oncology Research, Vol.24, No.5, pp. 371-380, 2016, DOI:10.3727/096504016X14685034103356

Abstract MicroRNA-21 (miRNA-21), a kind of short, noncoding RNAs, functioned as a tumor marker and was upregulated in renal cell carcinoma (RCC). However, the underlying mechanisms of miRNA-21 in RCC were uncertain. Therefore, the effects and mechanisms of miRNA-21 on the proliferation, differentiation, and apoptosis of cultured human RCC cells were further investigated in this study. After slicing miRNA-21 in RCC cells, the viability, mRNA expression of C/EBPα and PPARγ, caspase 3 activity, and protein expression of mTOR, STAT3, and pSTAT3 were determined. It was found that knockdown of miRNA-21 downregulated the optical density (OD) value More >

Yue-shen Wang^*1, Jing Tian^†1, Yong Han^†, Shu-mei Han^†, Sheng-bin Shi^†

Oncology Research, Vol.24, No.2, pp. 129-135, 2016, DOI:10.3727/096504016X14618564639213

Abstract We evaluated the efficacy and feasibility of the combination of gemcitabine plus vinorelbine in patients with platinum-based chemotherapy-refractory esophageal cancer. We enrolled 35 patients who received gemcitabine plus vinorelbine as second-line treatment after platinum-based chemotherapy failure between May 2009 and April 2012. Dosage: gemcitabine 1,000 mg/m² plus vinorelbine 25 mg/m² ; all drugs were administered on days 1 and 8 of a 21-day cycle, and this was continued until failure or unacceptable toxicity. A total of 125 cycles of treatment were administered, and all patients received at least two cycles of treatment (two to five cycles;… More >

Zhenfeng Jiang^*1, Lifen Yao^†1, Hongge Ma^†, Panpan Xu^†, Zhiyan Li^†, Mian Guo^‡, Jianhang Chen^*, Hongbo Bao^§, Shupei Qiao^¶, Yufang Zhao^¶, Jia Shen^#, Minwei Zhu^*, Carolyn Meyers^**, Guizhen Ma^††, Chuncheng Xie^*, Li Liu^*, Haiyang Wang^*, Wang Zhang^*, Qi Dong^†, Hong Shen^*, Zhiguo Lin^*

Oncology Research, Vol.25, No.6, pp. 1009-1019, 2017, DOI:10.3727/096504016X14813859905646

Abstract Pyroptosis is a type of proinflammatory programmed cell death mediated by caspase 1 activity and occurs in several types of eukaryotic tumor cells, including gliomas. MicroRNAs (miRNAs), small endogenous noncoding RNAs, have been demonstrated to be advantageous in glioma therapy. However, the question of whether miRNAs regulate pyroptosis in glioma remains unknown. The current study found that caspase 1 expression was substantially increased in both glioma tissues and glioma cell lines, U87 and T98G, while miR-214 expression was significantly downregulated. Luciferase reporter assay recognized caspase 1 as a target gene of miR-214. These findings demonstrate More >

Kewei Ren^*†‡, Zhen Li^*†‡, Yahua Li^*†‡, Wenzhe Zhang^*†‡, Xinwei Han^*†‡

Oncology Research, Vol.25, No.5, pp. 789-798, 2017, DOI:10.3727/096504016X14783677992682

Abstract Long noncoding RNA (lncRNA) taurine-upregulated gene 1 (TUG1) is involved in the development and carcinogenesis of various tumors, suggesting the diagnostic potential of TUG1 in these cancers. However, the exact role of TUG1 and its underlying mechanism in gastric cancer (GC) remain unknown. In this study, the expression of TUG1 and miR-145-5p in GC cell lines and nonmalignant gastric epithelial cell lines was detected by qRT-PCR. BGC-823 and SGC-7901 cells were transfected with si-TUG1, pcDNA 3.1-TUG1, miR-145-5p mimics, or matched controls. The biological function of TUG1 and miR-145-5p in GC cell proliferation and invasion in… More >

Lei Chen, Yuhai Wang, Jianqing He, Chunlei Zhang, Junhui Chen, Dongliang Shi

Oncology Research, Vol.26, No.9, pp. 1419-1428, 2018, DOI:10.3727/096504018X15178768577951

Abstract miR-152 and lncRNA H19 have been frequently implicated in various cellular processes including cell proliferation, invasion, angiogenesis, and apoptosis. However, the interaction between miR-152 and H19 in glioma has never been reported. RT-qPCR was used to examine the expression of miR-152 and H19 in human glioma cell lines and normal human astrocytes (NHAs). The interaction between miR-152 and lncRNA H19 was assessed by dual-luciferase reporter assay. MTT assay and Transwell invasion assay were used to determine the proliferation and invasion of U251 and U87 cells. A xenograft tumor experiment was performed to confirm the role… More >

Lixia Jiang^*1, Shaohua Shi^†1, Qiaofa Shi^‡, Huijuan Zhang^*, Yu Xia^§, Tianyu Zhong^*

Oncology Research, Vol.26, No.7, pp. 1055-1062, 2018, DOI:10.3727/096504018X15152056890500

Abstract HIF-2α knockdown inhibits proliferation, arrests the cell cycle, and promotes apoptosis and autophagy under hypoxic conditions in cervical cancer. However, the upstream regulatory mechanism of HIF-2α expression is unclear. MicroRNAs (miRNAs) degrade target mRNAs by binding to the 3'-untranslated region of mRNAs. In this study, we investigated the role of miRNAs in the regulation of HIF-2α expression in cervical cancer under hypoxic conditions. miRNAs regulating HIF-2α expression were predicted using TargetScan and miRanda and were determined in cervical cancer under hypoxic conditions by qRT-PCR. Additionally, the targeted regulation of HIF-2α by miR-519d-3p was evaluated by… More >

Dagang Li^*, Huizong Li^†, Yuping Yang^*, Le Kang^*

Oncology Research, Vol.26, No.4, pp. 537-546, 2018, DOI:10.3727/096504017X15009792179602

Abstract Long noncoding RNA urothelial carcinoma-associated 1 (lncRNA UCA1) has gained more attention in recent years due to its oncogenic roles in various cancers. MicroRNA-144 (miR-144) participates in the regulation of the growth of many cancer cells. This study investigated the interaction between lncRNA UCA1 and miR-144 in lung cancer cells. The potential downstream protein of miR-144 was also assessed. Our results found that lncRNA UCA1 was highly expressed in human lung cancer A549, H517, H4006, H1299, and H1650 cells compared to normal embryonic lung WI-38 and HEL-1 cells. Knockdown of lncRNA UCA1 significantly inhibited lung More >

Hai-Feng Zeng^*, Hai-Yan Qiu^†, Fa-Bo Feng^‡

Oncology Research, Vol.26, No.3, pp. 335-343, 2018, DOI:10.3727/096504017X14907375885605

Abstract Long noncoding RNAs (lncRNAs) have been verified to participate in various types of malignant tumors, including osteosarcoma (OS), which is the most common primary bone tumor with outstanding morbidity. Although an increasing number of lncRNAs have been reported to mediate the occurrence of OS, the potential mechanisms are still unclear. This study intends to uncover the mechanism by which lncRNA LINC01133 functions as an miRNA sponge to mediate OS tumorigenicity. In this study, we found that the expression level of LINC01133 was statistically upregulated in OS tumor tissue and cell lines compared to noncancerous tissues More >

Vinitha Richard, Rajesh Raju, Aswathy Mary Paul, Reshmi Girijadevi, Thankayyan Retnabai Santhosh Kumar, Madhavan Radhakrishna Pillai

Oncology Research, Vol.26, No.1, pp. 17-26, 2018, DOI:10.3727/096504017X14881490607028

Abstract This study is an integrated analysis of the transcriptome profile microRNA (miRNA) and its experimentally validated mRNA targets differentially expressed in the tumorigenic stem-like fraction of oral squamous cell carcinoma (OSCC). We had previously reported the coexistence of multiple drug-resistant tumorigenic fractions, termed side population (SP1, SP2, and MP2), and a nontumorigenic fraction, termed main population (MP1), in oral cancer. These fractions displayed a self-renewal, regenerative potential and expressed known stemness-related cell surface markers despite functional differences. Flow cytometrically sorted pure fractions of SP1 and MP1 cells were subjected to differential expression analysis of both… More >

XUHUI ZHAO^1,2, XIAOMIN HUANG¹, CHUNYAN DANG², XIA WANG¹, YUJIAO QI³, HONGLING LI^2,*

Oncology Research, Vol.32, No.5, pp. 999-1009, 2024, DOI:10.32604/or.2024.046679

Abstract Background: EBV-miR-BARTs exhibit significant relevance in epithelial tumors, particularly in EBV-associated gastric and nasopharyngeal cancers. However, their specific mechanisms in the initiation and progression of gastric cancer remain insufficiently explored. Material and Methods: Initially, EBV-miRNA-BART6-5p and its target gene SMAD4 expression were assessed in EBV-associated gastric cancer tissues and cell lines. Subsequent transfection induced overexpression of EBV-miRNA-BART6-5p in AGS and MKN-45, and downregulation in EBV-positive cells (SUN-719). The subsequent evaluation aimed to observe their impact on gastric cancer cell proliferation, migration, and glycolytic processes, with the TGF-β/SMAD4 signaling pathway value clarified using a TGF-β inhibitor. Results:… More >