Jianping Shi^*1, Yi Zhang^†1, Nuyun Jin^*, Yuqin Li^*, Shengtian Wu^*, Leiming Xu^†

Oncology Research, Vol.25, No.4, pp. 523-536, 2017, DOI:10.3727/096504016X14756282819385

Abstract Gastric carcinoma is one of the most common malignancies in men, and microRNA plays a critical role in regulating the signaling networks of gastric carcinoma tumorigenesis and metastasis. We first report the functional characteristics of miR-221-3p in gastric carcinoma. Quantification in gastric carcinoma cell lines and tumor samples reveals significantly increasing miR-221-3p expression. Moreover, a high level of miR-221-3p is correlated with a poor prognosis for gastric carcinoma patients. Ectopic miR-221-3p expression significantly promotes gastric carcinoma cell proliferation, invasion, and sphere formation, while silencing miR- 221-3p significantly inhibits these abilities in gastric carcinoma cells. Tests More >

YUE CAO, LIN HU, YISHU TANG^*

BIOCELL, Vol.47, No.7, pp. 1537-1548, 2023, DOI:10.32604/biocell.2023.027205

Abstract Backgrounds: Both hepatitis B virus X protein (HBx) and microRNA-221 (miR-221) have been implicated in the development of hepatitis B virus (HBV)-related hepatocellular carcinoma (HCC). The present study demonstrates that HBx promotes HCC cell proliferation via the C-X-C motif chemokine ligand 12-C-X-C chemokine receptor type 4 (CXCL12-CXCR4) axis. We predict that HBx/miR-221-mediated CXCL12/CXCR4 signaling induces NKT cells to promote HBV-related HCC. Methods: After miR-221 mimic, miR-221 mimic negative control, miR-221 inhibitor, miR-221 inhibitor negative control were transfected into cells, the expression of CXCL12 and miR-221 was detected by qPCR and western blot. Then we constructed… More >