GUIBIN ZHONG^1,2,#, SHURONG WANG^3,#, YUJIN HE⁴, DAMING FENG¹, KE WEI¹, YANQIU YANG¹, JIANWEI CHEN^1,2,*, JUNLING CHEN^1,*

BIOCELL, Vol.48, No.6, pp. 1001-1008, 2024, DOI:10.32604/biocell.2024.050294

Abstract Background: Hypertrophy of the ligamentum flavum (HLF) is a common contributor to spinal stenosis which results in significant neurological impairments. Circular RNA (circRNA) circ_0003609 has been linked to HLF; however, the exact mechanism by which it causes this disease is unclear. Methods: Circ_0003609 expressions were regulated in HLF cells by overexpression vectors and RNA interference. Cell proliferation and fibrosis-related gene expression were checked by the Cell Counting Kit-8 (CCK-8) assay and western blotting. CircBank’s prediction of the association between miR-155 and circ_0003609 was supported by a dual-luciferase reporter experiment. The function of the miR-155/sirtuin 1 More >

Fu-qiang Zhu^*1, Li Zeng^†1, Na Tang^*, Ya-ping Tang^‡, Bo-ping Zhou^*, Fang-fang Li^*, Wei-gang Wu^*, Xiao-bing Zeng^*, Shu-song Peng^*

Oncology Research, Vol.24, No.6, pp. 415-427, 2016, DOI:10.3727/096504016X14685034103473

Abstract Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin’s lymphoma in the adult population, and treatment of DLBCL is still unfavorable. Therefore, there is an urgent requirement to investigate the molecular mechanisms underlying DLBCL tumorigenesis. To study the potential function of microRNA-155 (miR-155) involved in the regulation of lymphoma, we monitored lymphoma cell behavior including proliferation, cell cycle, and apoptosis using CCK-8 and flow cytometry analysis. Real-time PCR was used to detect the expression levels of miR-155 in 118 lymphoma patients’ tissues, and Western blot was also used to analyze the expression level of… More >

Jianing Xu^*†, Qiyu Bo^‡, Xiang Zhang^§, Dapeng Lei^†, Jue Wang^*, Xinliang Pan^†

Oncology Research, Vol.28, No.3, pp. 311-319, 2020, DOI:10.3727/096504020X15801233454611

Abstract Hypopharyngeal squamous cell carcinoma (HSCC) remains one of the most lethal malignancies in the head and neck. Long noncoding RNA (lncRNA) HOXA11-AS is proven to function as an oncogene and a therapeutic target in various tumors. Our previous study and others have demonstrated that HOXA11-AS is one of the most upregulated lncRNAs in HSCC. However, the role of HOXA11-AS in HSCC has not yet been identified. The current study demonstrated that the expression of HOXA11-AS was significantly upregulated in HSCC tumors and was positively associated with lymph node metastasis. Moreover, functional experiments revealed that HOXA11-AS More >

ZHIHENG LIU^1,2, ZHEN SUN³, ZHONGYUAN WAN⁴, HAIQIANG WANG^2,*

BIOCELL, Vol.46, No.2, pp. 511-517, 2022, DOI:10.32604/biocell.2021.016194

Abstract We unraveled the expression profiles of coding-noncoding RNAs in intervertebral disc degeneration (IDD). However, it remains elusive regarding miR-155 expression in peripheral blood mononuclear cells (PBMCs) and local extracellular space in IDD. The study aimed for investigating the miR-155 expression of PBMCs, extracellular miRNAs (ex-miRNAs) of human nucleus pulposus (NP) tissues, and morphological changes of cell death in the IDD process. Here, we harvested peripheral blood and NP samples from scoliosis and IDD patients as control and degenerative groups, respectively. Then standard Ficoll density-gradient centrifugation was used to isolate PBMCs. The two subpopulations of PBMCs… More >