Dongliang Zhu1,*, Jun Yang2, Jiaxin Xu3
Oncologie, Vol.24, No.3, pp. 471-482, 2022, DOI:10.32604/oncologie.2022.024062
- 19 September 2022
Abstract G-protein coupled estrogen receptor (GPER) is a transmembrane receptor that mediates non-genomic effects of
estrogen. This study aimed to investigate the role of GPER in the stemness formation and malignancies in triple
negative breast cancer (TNBC) cells. Spheroids of MDA-MB-468 cells were induced by mammosphere culture,
and the proportion of the CD44+
/CD24−/low stem cell subpopulation was detected. Malignant characteristics,
expression of GPER and stemness-related markers, and tumorigenesis in a xenograft assay were compared
between the mammospheres and adherent cultured cells. The impacts of 17β-estradiol (E2) and the GPER-specific
antagonist G15 were studied in in vitro assays.… More >
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