WENHAO XU^1,#, HAOMING LI^2,#, YASIR HAMEED³, MOSTAFA A. ABDEL-MAKSOUD⁴, SAEEDAH MUSAED ALMUTAIRI⁴, AYMAN MUBARAK⁴, MOHAMMED AUFY⁵, WAEL ALTURAIKI⁶, ABDULAZIZ J. ALSHALANI⁶, AYMAN M. MAHMOUD^7,*, CHEN LI^8,*

Oncology Research, Vol.31, No.5, pp. 819-831, 2023, DOI:10.32604/or.2023.029414

Abstract N6-methyladenosine methylation (m6A) is a common type of epigenetic alteration that prominently affects the prognosis of tumor patients. However, it is unknown how the m6A regulator affects the tumor microenvironment (TME) cell infiltration in adrenocortical carcinoma (ACC) and how it affects the prognosis of ACC patients yet. The m6A alteration patterns of 112 ACC patients were evaluated, furthermore, the association with immune infiltration cell features was investigated. The unsupervised clustering method was applied to typify the m6A alteration patterns of ACC patients. The principal component analysis (PCA) technique was taken to create the m6A score to assess the alteration pattern… More >

YANPING JIN¹, JIANPING QIU¹, XIUFANG LU¹, YAN MA¹, GUOWEI LI^2,*

Oncology Research, Vol.31, No.2, pp. 169-179, 2023, DOI:10.32604/or.2023.027815

Abstract Previous study revealed that ferritin heavy chain-1 (FTH1) could regulate ferritinophagy and affect intracellular Fe²⁺ content in various tumors, while its N6-methyladenosine (m6A) RNA methylation was closely related the prognosis of ovarian cancer patients. However, little is known about the role of FTH1 m6A methylation in ovarian cancer (OC) and its possible action mechanisms. In this study we constructed FTH1 m6A methylation regulatory pathway (LncRNA CACNA1G-AS1/IGF2BP1) according to related bioinformatics analysis and research, through clinical sample detections we found that these pathway regulatory factors were significantly up-regulated in ovarian cancer tissues, and their expression levels were closely related to the… More >

JING JIN^1,#, XINGHUA WANG^2,#, XIAOXIAO ZHENG³, JIAHUA LAN³, LI ZHENG³, YING CAI³, HUI CHEN⁴, HONGWEI WANG^5,*, LIFANG ZHENG^6,*

BIOCELL, Vol.47, No.3, pp. 619-626, 2023, DOI:10.32604/biocell.2023.026016

Abstract Ischemic cerebrovascular disease is a leading cause of death globally and is often exacerbated by cerebral ischemic/reperfusion injury (CIRI). The exact mechanisms underlying I/R injury are unclear. In this study, we aimed to determine the role of m⁶A-modified methylase complex methyltransferase-like 3 (METTL3) in cerebral ischemia-reperfusion (I/R) injury. We found that m6A and METTL3 levels increased in OGD/RX-induced mouse astrocytes-cerebellar (MA-C) and the brain of middle cerebral artery occlusion (MCAO) model mice. METTL3 siRNA treatment reduced OGD-RX-induced MAC cell viability and proliferation, which increased with METTL3 over-expression. Flow cytometry analysis showed that silencing METTL3 significantly enhanced OGD/RX-induced MAC apoptosis, which… More >

CANGZHI JIA¹, DONG JIN¹, XIN WANG¹, QI ZHAO^2,*

BIOCELL, Vol.46, No.4, pp. 1105-1121, 2022, DOI:10.32604/biocell.2022.016655

Abstract N⁶-Methyladenine is a dynamic and reversible post translational modification, which plays an essential role in various biological processes. Because of the current inability to identify m6A-containing mRNAs, computational approaches have been developed to identify m6A sites in DNA sequences. Aiming to improve prediction performance, we introduced a novel ensemble computational approach based on three hybrid deep neural networks, including a convolutional neural network, a capsule network, and a bidirectional gated recurrent unit (BiGRU) with the self-attention mechanism, to identify m6A sites in four tissues of three species. Across a total of 11 datasets, we selected different feature subsets, after optimized… More >