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  • Open Access

    ARTICLE

    Forkhead box protein O1 (FoxO1) regulates lipids metabolism and cell proliferation mediated by insulin and PI3K-Akt-mTOR pathway in goose primary hepatocytes

    RONGXUE WEI1, CHUNCHUN HAN1,*, FENGJIANG YE1, SHOUHAI WEI2, FANG HE1, HEHE LIU1, LIANG LI1, HONGYONG XU1, SHENQIANG HU1, XIANYIN ZENG2

    BIOCELL, Vol.46, No.1, pp. 171-183, 2022, DOI:10.32604/biocell.2022.015409

    Abstract In order to explore the role of forkhead box protein O1 (FoxO1) in the lipid metabolism and cell proliferation, goose primary hepatocytes were isolated and incubated with insulin or PI3K-Akt-mTOR pathway dual inhibitor NVP-BEZ235, and then transfected with FoxO1 interference plasmid. The related parameters of lipid metabolism and cell proliferation were measured. The results firstly showed that FoxO1 interference increased the intracellular TG and lipids concentration (P < 0.05); and increased the proliferative index (PI), cell DNA synthesis, protein expression of Cyclin D1 in goose primary hepatocytes (P < 0.05). Secondly, the co-treatment of insulin and FoxO1 interference increased the… More >

  • Open Access

    ARTICLE

    Benzyl Amino Purine and Gibberellic Acid Coupled to Nitrogen-Limited Stress Induce Fatty Acids, Biomass Accumulation, and Gene Expression in Scenedesmus Obliquus

    Hans Christian Correa-Aguado1,3, Gloria Viviana Cerrillo-Rojas1, Alejandro Rocha-Uribe2, Ruth Elena Soria-Guerra2, José Francisco Morales-Domínguez1,*

    Phyton-International Journal of Experimental Botany, Vol.90, No.2, pp. 515-531, 2021, DOI:10.32604/phyton.2021.013619

    Abstract The need for renewable energy sources makes microalgae an essential feedstock for biofuels production. The molecular aspects and the response to nitrogen (N)-limited conditions with a phytohormone stimulus in microalgae have been slightly explored. In this work, Scenedesmus obliquus was used as a study model to analyze the effect of benzyl amino purine (BAP) and gibberellic acid (GA) coupled to nitrogen limitation on cell growth, biomass and fatty acids. The selected 10-5 M BAP increased the biomass by 1.44-fold, and 10-6 M GA by 1.35-fold. The total lipids also increased by 2.8 and 1.11-fold, respectively. The 10-5 M BAP and… More >

  • Open Access

    ARTICLE

    Storage lipids and proteins of Euterpe edulis seeds

    VÍCTOR PANZA1,2, DARIO PIGHIN3, VERÓNICA LÁINEZ2, RICARDO J. POLLERO4, SARA MALDONADO1,2,*

    BIOCELL, Vol.33, No.2, pp. 99-106, 2009, DOI:10.32604/biocell.2009.33.099

    Abstract Comparative studies on fatty acid and protein composition of the endosperm and embryo of palmito (Euterpe edulis Martius) were conducted using gas-liquid chromatography and sodium dodecyl sulfate–polyacrylamide gel electrophoresis. On a dry weight basis, the embryo contained extremely lower amounts of lipids and proteins than did the endosperm, which was associated with the scarce lipid and protein bodies previously reported in axis and cotyledon. The fatty acid composition also exhibited differences between both tissues: (I) the fatty acid diversity was greater in embryo than in endosperm; (II) embryo and endosperm contained predominantly linoleic, palmitic, oleic and stearic acids even though… More >

  • Open Access

    ABSTRACT

    Minireview: Lipoproteins from plasma and perivelline fluid of the apple snail Pomacea canaliculata

    H. Heras, R.J. Pollero

    BIOCELL, Vol.26, Suppl.S, pp. 111-118, 2002

    Abstract This article has no abstract. More >

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