Boxue Chen^1,#, Hongda Wang^1,#, Meiyu Liu¹, Wandi Hu¹, Yuexin Qian¹, Jiali Wang¹, Jie Liu¹, Xue Li¹, Jing Wang², Wenzhi Yang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.5, pp. 1073-1088, 2022, DOI:10.32604/phyton.2022.019399 - 24 January 2022

Abstract Herbal components characterization represents a challenging task because of the co-existing of multiple classes of naturally occurring compounds with wide spans of polarity, molecular mass, and the ubiquitous isomerism. The root and rhizome of Salvia miltiorrhiza have been utilized as a reputable traditional Chinese medicine Salviae Miltiorrhizae Radix et Rhizoma (Dan-Shen) in the treatment of cardiovascular disease. Herein, a dimension-enhanced ultra-high performance liquid chromatography/ion mobility/quadrupole time-of-flight mass spectrometry approach in combination with intelligent peak annotation workflows was established aimed to rapidly characterize the multicomponents from S. miltiorrhiza. Due to the sufficient optimization, satisfactory chromatography separation was enabled… More >

Simiao Wang^1,#, Xue Li^1,#, Boxue Chen¹, Shitong Li¹, Jiali Wang¹, Jing Wang², Mingshuo Yang³, Xiaoyan Xu¹, Hongda Wang¹, Wenzhi Yang^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.3, pp. 541-567, 2022, DOI:10.32604/phyton.2022.018571 - 26 October 2021

Abstract The complex composition of herbal metabolites necessitates the development of powerful analytical techniques aimed to identify the bioactive components. The seeds of Descurainia sophia (SDS) are utilized in China as a cough and asthma relieving agent. Herein, a dimension-enhanced integral approach, by combining ultra-high performance liquid chromatography/ion mobility-quadrupole time-of-flight mass spectrometry (UHPLC/IM-QTOF-MS) and intelligent peak annotation, was developed to rapidly characterize the multicomponents from SDS. Good chromatographic separation was achieved within 38 min on a UPLC CSH C18 (2.1 × 100 mm, 1.7 μm) column which was eluted by 0.1% formic acid in water (water phase)… More >