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  • Open Access

    ARTICLE

    Phenolic Profile and Volatiles of in vitro Propagated Lavandula angustifolia Mill. Seedlings

    Mir Abdullatif Yahya1, Fatma Tunali1, Dilek Killi2,*, Atalay Sökmen2

    Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 427-444, 2024, DOI:10.32604/phyton.2024.046271 - 28 March 2024

    Abstract An effective in vitro propagation protocol was designed for Lavandula angustifolia Miller, a medicinal aromatic plant that is a prominent source of volatile organic compounds (VOCs). Murashige and Skoog media were supplemented with various concentrations of Plant Growth Regulators (PGRs), and the growth parameters of the nodal segments were examined. Nodal explants formed callus when they were supplemented with 2 mg/L of 6-Benzylaminopurine (BAP). The superior hormonal concentration of Murashige and Skoog (MS) media for the proliferation of shoots from callus cultures (39.33%) was 5 mg/L of 2-Isopentenyl adenine (2iP), and the favorable media for the growth… More >

  • Open Access

    ARTICLE

    In vitro plant regeneration via indirect organogenesis from different explants of Lathyrus sativus L. and Lathyrus cicera L.

    Li RS1, YJ Tao2, FJ Liu2, X Hu2, QL Xu2, KY Li2

    Phyton-International Journal of Experimental Botany, Vol.85, pp. 87-93, 2016, DOI:10.32604/phyton.2016.85.087

    Abstract The grass pea (Lathyrus sativus L.) and flatpod peavine (Lathyrus cicera L.) are the most economically important and widely cultivated Lathyrus species. However, their utilization is limited due to the presence of their endogenous toxin β-N-oxalyl-L-α, β-diaminopropionic acid (β-ODAP). Thus, a β-ODAP free variety should be developed through some plant breeding technique like either mutational breeding or genetic-manipulation. In this circumstance, the plant regeneration of Lathyrus species becomes a bottleneck. In the present study, an efficient system for in vitro regeneration of L. sativus with high β-ODAP levels, and L. cicera with low β-ODAP levels, was developed from different explants (axillary… More >

  • Open Access

    ARTICLE

    Influence of genotype and explant source on indirect organogenesis by in vitro culture of leaves of Melia azedarach L.

    S.K. VILA*, H.Y. REY, L.A. MROGINSKI

    BIOCELL, Vol.28, No.1, pp. 35-41, 2004, DOI:10.32604/biocell.2004.28.035

    Abstract In vitro regeneration of shoots from leaf explants of the Paradise tree (Melia azedarach L.) was studied. Three different portions (proximal portion, distal portion and rachis of the leaflets) of three developmental stages (folded, young still expanding and completely expanded) of leaves of 10 – 15 years old plants of seven genotypes were cultured on Murashige and Skoog (1962) medium (MS) supplemented with 1mg.l-1 benzylaminopurine (BAP) + 0.1mg.l-1 kinetin (KIN) + 3 mg.l-1 adenine sulphate (ADS).
    The rachis of the leaflets of the completely expanded leaves was found to be the most responsive tissue, in most of the More >

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