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  • Open Access

    ARTICLE

    Physiological Response Mechanism and Drought Resistance Evaluation of Passiflora edulis Sims under Drought Stress

    Binyang Zhao1, Fengchan Wu2, Guojun Cai3, Peiyu Xi2, Yulin Guo2, Anding Li2,*

    Phyton-International Journal of Experimental Botany, Vol.93, No.6, pp. 1345-1363, 2024, DOI:10.32604/phyton.2024.050950

    Abstract In order to explore the response mechanism of Passiflora edulis Sims to drought stress, the changes in morphological and physiological traits of Passiflora edulis Sims under different drought conditions were studied. A total of 7 germplasm resources of Passiflora edulis Sims were selected and tested under drought stress by the pot culture method under 4 treatment levels: 75%–80% (Control, CK) of maximum field water capacity, 55%–60% (Light Drought, LD) of maximum field water capacity, i.e., mild drought, 40%–45% (Moderate Drought, MD) of maximum field water capacity, i.e., moderate drought and 30%–35% (Severe Drought, SD) of maximum field water… More >

  • Open Access

    ARTICLE

    GNAS mutations suppress cell invasion by activating MEG3 in growth hormone–secreting pituitary adenoma

    CHAO TANG1,#, CHUNYU ZHONG2,#, JUNHAO ZHU1, FENG YUAN1, JIN YANG1, YONG XU3,*, CHIYUAN MA1,3,4,5,*

    Oncology Research, Vol.32, No.6, pp. 1079-1091, 2024, DOI:10.32604/or.2024.046007

    Abstract Approximately 30%–40% of growth hormone–secreting pituitary adenomas (GHPAs) harbor somatic activating mutations in GNAS (α subunit of stimulatory G protein). Mutations in GNAS are associated with clinical features of smaller and less invasive tumors. However, the role of GNAS mutations in the invasiveness of GHPAs is unclear. GNAS mutations were detected in GHPAs using a standard polymerase chain reaction (PCR) sequencing procedure. The expression of mutation-associated maternally expressed gene 3 (MEG3) was evaluated with RT-qPCR. MEG3 was manipulated in GH3 cells using a lentiviral expression system. Cell invasion ability was measured using a Transwell assay, and epithelial–mesenchymal transition… More >

  • Open Access

    REVIEW

    Do tensile and shear forces exerted on cells influence mechanotransduction through stored energy considerations?

    FREDERICK H. SILVER1,2,*, TANMAY DESHMUKH2

    BIOCELL, Vol.48, No.4, pp. 525-540, 2024, DOI:10.32604/biocell.2024.047965

    Abstract All tissues in the body are subjected externally to gravity and internally by collagen fibril and cellular retractive forces that create stress and energy equilibrium required for homeostasis. Mechanotransduction involves mechanical work (force through a distance) and energy storage as kinetic and potential energy. This leads to changes in cell mitosis or apoptosis and the synthesis or loss of tissue components. It involves the application of energy directly to cells through integrin-mediated processes, cell-cell connections, stretching of the cell cytoplasm, and activation of the cell nucleus via yes-associated protein (YAP) and transcriptional coactivator with PDZ-motif… More >

  • Open Access

    ARTICLE

    The regulatory role of melatonin in pituitary thyroid-stimulating hormone synthesis through casein kinase 1α

    BINGJIE WANG1, YEWEN ZHOU1,2,3, TONGJUAN NIU1, MENGQING YIN1, SHENG CUI1,2,3,*

    BIOCELL, Vol.48, No.2, pp. 327-338, 2024, DOI:10.32604/biocell.2023.044630

    Abstract Introduction: The regulation of thyroid-stimulating hormone (TSH) synthesis involves neurotransmitters, with melatonin being a subject of ongoing debate. TSH transcription, synthesis, and secretion from the pituitary pars distalis (PD) is primarily regulated in a photoperiodic manner by thyrotropin-releasing hormone (TRH). In contrast, in the pituitary pars tuberalis (PT), mRNA transcription and alpha/beta chain synthesis, but not secretion, of a TSH-like product is regulated by melatonin. Conversely, non-photoperiodic melatonin might also affect the secretion of a TSH-like product from the PT. Nevertheless, the impact of exogenous melatonin on the underlying PD-TSH synthesis remains unclear. Casein kinase… More >

  • Open Access

    ARTICLE

    Transcriptome Analysis of Molecular Mechanisms Underlying Phenotypic Variation in Phaseolus vulgaris Mutant ‘nts’

    Limin Yin#, Chang Liu#, Zicong Liang, Dajun Liu, Guojun Feng, Zhishan Yan*, Xiaoxu Yang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 2981-2998, 2023, DOI:10.32604/phyton.2023.043151

    Abstract The phenotype of a common bean plant is often closely related to its yield, and the yield of plants with reduced height or poor stem development during growth is low. Mutants serve as an essential gene resource for common bean breeding genetic research. Although model plants and crops are studied to comprehend the molecular mechanisms and genetic basis of plant phenotypes, the molecular mechanism of phenotypic variation in common beans remains underexplored. We here used the mutant ‘nts’ as material for transcriptome sequencing analysis. This mutant was obtained through 60Co-γ irradiation from the common bean variety… More >

  • Open Access

    ARTICLE

    Characterization of Endophytic Microorganisms of Rice (Oryza sativa L.) Potentials for Blast Disease Biocontrol and Plant Growth Promoting Agents

    Shugufta Parveen1, Fayaz A. Mohiddin2,*, M. Ashraf Bhat3, Zahoor Ahmed Baba4, Fehim Jeelani5, M. Anwar Bhat6, Sajad Un Nabi7, Burhan Hamid2, Saba Bandey8, Farhanaz Rasool9, Zakir Amin1, Ibrahim Al-Ashkar10,*, Muhammad Adnan11, Ayman El Sabagh12

    Phyton-International Journal of Experimental Botany, Vol.92, No.11, pp. 3021-3041, 2023, DOI:10.32604/phyton.2023.030921

    Abstract One hundred twenty-five endophytic microorganisms were isolated from the roots, stems, and leaves of four prominent rice cultivars growing in temperate regions. Their potential to combat rice blast disease and promote plant growth was investigated. The dual culture tests highlighted the strong antagonistic activity of five fungal (ranging from 89%–70%) and five bacterial (72%–61%) endophytes. Subsequent examination focused on volatile compounds produced by selected isolates to counter the blast pathogen. Among these, the highest chitinase (13.76 µg mL−1) and siderophore (56.64%), was exhibited by Aspergillus flavus, and the highest HCN production was shown by Stenotrophomonas maltophilia (36.15 µM… More >

  • Open Access

    REVIEW

    Adventitious Root Regeneration: Molecular Basis and Influencing Factors

    Lulu Zhi, Xiangyang Hu*

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2825-2840, 2023, DOI:10.32604/phyton.2023.030912

    Abstract Plant regeneration is a self-repair of the plant body in response to adverse conditions or damaged structures, and root regeneration allows the plant body to better adapt to its environment by supplementing the roots’ structure. Previous research has shown that adventitious roots can be made to occur from scratch in two ways. Studies that simulate adventitious root regeneration through natural conditions allow the regeneration process to be broadly divided into three stages: the perception of early signals, the massive accumulation of auxin, and the transformation of cell fate. The strength of regeneration, in turn, is More >

  • Open Access

    ARTICLE

    Effects of Root Cutting on Morphological Characteristics and Endogenous Hormone Levels of Quercus variabilis Seedlings

    Ruoyi Ni#, Hao Dou#, Shuwen Bi, Tiantian Wang, Jin’e Quan*, Xitian Yang*

    Phyton-International Journal of Experimental Botany, Vol.92, No.10, pp. 2907-2920, 2023, DOI:10.32604/phyton.2023.030689

    Abstract The objective of this study was to investigate the effects of root cutting stress on the dynamic changes of endogenous hormone content and growth characteristics of Quercus variabilis roots, and to explore the physiological role of endogenous hormones in regulating root-crown interactions in the short term. The morphological characteristics and endogenous hormone contents of normal roots (no root cutting, CK) and cut roots (cut by 1/3 of the length of the main root, RP) were determined by liquid chromatography, which was combined with mass spectrometry at different levels of different developmental stages. The results showed that… More >

  • Open Access

    REVIEW

    The effects of hormone-mediated PI3K/AKT signaling on spermatogenesis in Sertoli cells

    YANSHUANG ZHAO, WANXI YANG*

    BIOCELL, Vol.47, No.8, pp. 1709-1725, 2023, DOI:10.32604/biocell.2023.030379

    Abstract The phosphoinositide-3-kinase/Akt (PI3K/AKT) signaling pathway is crucial for Sertoli cell development and completing spermatogenesis. Its main role is to promote proliferation and inhibit apoptosis. Many factors activate the PI3K/AKT pathway, like hormones, such as follicle stimulating hormone (FSH), androgen, estrogen, insulin to name a few. Many of these factors have receptors inside or on the surface of Sertoli cells (SCs). This review summarizes how these hormones directly regulate the PI3K/AKT signaling pathway in SCs, which in turn affects SC proliferation and differentiation. Further, hormone-mediated PI3K/AKT signaling also stimulates SC secretion, which is essential for germ More >

  • Open Access

    ARTICLE

    Inoculation of Chlorella and Food Waste Improves the Physio-Morphological Features of Red Pepper by Regulating Activating Antioxidant Defense System

    Sang-Mo Kang1,#, Shifa Shaffique1,#, Muhammad Imran2,#, Su-Mi Jeon3, Shabir Hussain Wani5, Muhammad Aaqil Khan4, Peter Odongkara1, Eun-Hae Kwon1, Yosep Kang1, Joon-Ik Son6, Won-Chan Kim1,*, In-Jung Lee1,*

    Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2699-2711, 2023, DOI:10.32604/phyton.2023.028224

    Abstract Food waste is recognized as a valuable source for potential agricultural applications to supply organic matter and nutrients to arable soil. However, the information on the combined application of food waste and the plant growth-promoting bacterial strain, Chlorella, related to plant metabolic features and sodium chloride content in arable soil is limited. The present study was conducted to investigate the exogenous application of food waste along with Chlorella, which improved the physio-morphological features of red pepper. Our results revealed that this combination enhanced the organic matter in the soil, ultimately improving the fertility rate of the More >

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