HONGYANG LI1,2, CHAN WANG4, SHIWEI LIU1, YONGLONG GUO1,3,4,*, JIANSU CHEN1,3,4,*
BIOCELL, Vol.46, No.7, pp. 1705-1716, 2022, DOI:10.32604/biocell.2022.018442
Abstract Human adipose stem cells (hADSCs) are reliable sources for cell therapy. However, the clinical applications are limited by the decrease in activity during in vitro culture. We used a knockout serum replacement (KSR) medium, Eppendorf (EP) tube culture, and a simulated microgravity (SMG) culture system to establish hADSC spheroids. We found that hADSCs aggregated and formed spheroids in the KSR culture medium. The EP tube culture method revealed many biological cell characteristics, such as good cell viabilities, rough surfaces, polar growth, fusion phenomenon, and injectability. The findings show its advantages for hADSCs spherical cultures. When cultured in SMG, hADSC spheroids… More >
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