Zhenbing Qiao^1,2, Mengyun Chen^1,2, Wenjun Ma^1,2, Juan Zhao^1,2, Jiaju Zhu^1,2, Kaikai Zhu^1,2, Pengpeng Tan^1,2, Fangren Peng^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2349-2365, 2024, DOI:10.32604/phyton.2024.056655 - 30 September 2024

Abstract Ammonium nitrogen (NH₄⁺-N) is one of the main forms of nitrogen absorbed and utilized by plants, and mastering the regulatory mechanism of plant ammonium assimilation is a key way to improve the efficiency of plant nitrogen utilization. Glutamine synthetase (GS) and glutamate synthase (GOGAT), two key enzymes for ammonium assimilation, have rarely been studied in pecan. In this study, GS and GOGAT family members of pecan were identified and analyzed using bioinformatics methods. The results indicated that 6 GS and 4 GOGAT genes were identified. The cis-acting elements can be broadly categorized into light-responsive, hormone-responsive, and stress-responsive elements.… More >

Manoochehr MESSRIPOUR¹, Azadeh MESRIPOUR²

BIOCELL, Vol.43, No.1, pp. 37-40, 2019, DOI:10.32604/biocell.2019.05865

Abstract DNA damage and the enzyme poly (ADP-ribose) polymerase (PARP) associated with the pathogenesis of numerous age-related neurodegenerative disorders. Astrocytes play crucial roles in both support metabolic functions and cell viability of the brain. PARP regulates DNA damage and repair in the brain cells. In this study PARP activity and DNA strand break were investigated in the astrocytes isolated from young and aged rat brain. Three and 30-month-old rats were killed by decapitation and brains were removed onto an ice cooled glass plate. Astrocytes were isolated by sucrose density gradient centrifugation and glutamine synthetase (GS) served… More >