Bo Hwan Kim^1,#, Sheikh Mansoor^2,#, Gyung Deok Han³, Ji Eun Park⁴, Wook Kim^1,*, Yong Suk Chung^2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.9, pp. 2339-2347, 2024, DOI:10.32604/phyton.2024.056439 - 30 September 2024

Abstract Recognizing the variation of genetic resources is the first step in selection. One of the most important variations in grain crops is the uniformity of seed grain weight, which can be converted into seed size. However, it has been challenging since it needs high labor costs and time to measure it on a large scale. The current study used an image analysis technique to measure the grain seed area of about 100 seeds per accession with 64 germplasm of Tartary buckwheat (Fagopyrum tataricum) to study variation among and within them. To understand the nature of variation, More >

Haoxue Wu^#, Xiaohan Zhang^§,#, Xiaoyu Chen, Kang Li, Aixia Xu, Zhen Huang, Jungang Dong, Chengyu Yu^*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 627-640, 2024, DOI:10.32604/phyton.2024.050321 - 28 March 2024

Abstract Genetic manipulation (either restraint or enhancement) of the biosynthesis pathway of α-linolenic acid (ALA) in seed oil is an important goal in Brassica napus breeding. B. napus is a tetraploid plant whose genome often harbors four and six homologous copies, respectively, of the two fatty acid desaturases FAD2 and FAD3, which control the last two steps of ALA biosynthesis during seed oil accumulation. In this study, we compared their promoters, coding sequences, and expression levels in three high-ALA inbred lines 2006L, R8Q10, and YH25005, a low-ALA line A28, a low-ALA/high-oleic-acid accession SW, and the wildtype ZS11. The expression… More >

Riaz Ahmad^1,*, Hafiza Muniba Din Muhammad², Safina Naz², Meryam Manzoor², Muhammad Ahsan Altaf³

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1539-1553, 2023, DOI:10.32604/phyton.2023.026672 - 09 March 2023

Abstract Salinity is one of the major constraints reducing plant growth and yield. Irrigation with poor quality and brackish water to orchards is a major cause of stunted growth and low yield. The salt tolerance mechanism is one of the complicated genomic characters that is very problematic to develop in fruit trees and becomes much more severe at any growth and developmental stage. Osmotic stress and hormonal imbalances are major constraints causing low biomass production. Fruit tree tolerance/sensitivity is chiefly based on the activation of a defense system comprised of super-oxidase dismutase (SOD), peroxidase (POD) and… More >

Liyan Wu¹, Jie Cheng^1,2, Yaju Gong¹, Rui Bao¹, Zhibin Li¹, Min Gui^1,*, Guanghui Du^2,*

Phyton-International Journal of Experimental Botany, Vol.92, No.2, pp. 591-609, 2023, DOI:10.32604/phyton.2022.023481 - 12 October 2022

Abstract In this study, wild eggplant germplasm No. M239, which is highly susceptible to Verticillium wilt, was used as the experimental material. The physiological and biochemical indices (SOD, PAL, MDA and soluble protein) of M239 roots were measured at different times (0, 12, 24, 36, 48, 60 and 72 h) post inoculation with Verticillium dahliae, and the key time points for the M239 response to Verticillium wilt infection were screened. Then, RNA-Seq technology was used to screen the differentially expressed genes (DEGs) in M239 roots at 0, 12 and 48 h post-inoculation (hpi). The transcriptional results of M239… More >

KHANDAKAR ABU MD MOSTAFIZAR RAHMAN^1,2, ABDUL SHUKOR JURAIMI^2,*, MD. REZWAN MOLLA³, MUHAMMAD ASYRAF MD HATTA⁴, ZULKEFLY BIN SULAIMAN⁵, SHAMIMA SULTANA⁶, AHMED GABER⁷, BENUKAR BISWAS⁸, AKBAR HOSSAIN^9,*

BIOCELL, Vol.47, No.1, pp. 227-237, 2023, DOI:10.32604/biocell.2023.025135 - 26 September 2022

Abstract Every breeding program that aims to create new and improved cultivars with desired traits mostly relies on information related to genetic diversity. Therefore, molecular characterization of germplasms is important to obtain target cultivars with desirable traits. Sweet potato [Ipomoea batatas (L.) Lam] is widely considered the world’s most important crop, with great diversity in morphological and phenotypic traits. The genetic diversity of 20 sweet potato germplasms originating from Bangladesh, CIP, Philippines, Taiwan, and Malaysia were compared, which was accomplished by genetic diversity analysis by exploring 20 microsatellite DNA markers for germplasm characterization and utilization. This information… More >

Jing Qu¹, Shuang Liu², Peng Jiao², Zhenzhong Jiang², Jianbo Fei², Shuyan Guan^1,*, Yiyong Ma^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.8, pp. 1709-1719, 2022, DOI:10.32604/phyton.2022.018854 - 14 April 2022

Abstract To develop cold-tolerant maize germplasms and identify the activation of INDUCER OF CRT/DRE-BINDING FACTOR EXPRESSION (ICE1) expression in response to cold stress, RT-PCR was used to amplify the complete open reading frame sequence of the ICE1 gene and construct the plant expression vector pCAMBIA3301-ICE1-Bar. Immature maize embryos and calli were transformed with the recombinant vector using Agrobacterium tumefaciens-mediated transformations. From the regenerated plantlets, three T1 lines were screened and identified by PCR. A Southern blot analysis showed that a single copy of the ICE1 gene was integrated into the maize (Zea mays L.) genomes of the three T1 generations.… More >

Fen Zhang¹, Weili Tian¹, Lu Cen¹, Litang Lv², Xiaofang Zeng¹, Yulu Chen¹, Yichen Zhao^2,*, Yan Li^1,*

Phyton-International Journal of Experimental Botany, Vol.91, No.4, pp. 791-809, 2022, DOI:10.32604/phyton.2022.018104 - 09 December 2021

Abstract Duyun Maojian tea is a famous tea in China. In this study, the specific-locus amplified fragment (SLAF) sequencing method was used to analyze the population structure and conduct a genome-wide association study (GWAS) of 2 leaf traits of 123 tea plants in Qiannan, China. A total of 462,019 SLAF tags and 11,362,041 single-nucleotide polymorphism (SNP) loci were obtained. The results of phylogenetic tree analysis, cluster analysis, and principal component analysis showed that 123 tea germplasms were clustered into three groups, and the heterozygosity rates for Groups I, II, and II were 0.206, 0.224, and 0.34,… More >

DEBI RANI DATTA¹, MOHD RAFII YUSOP^1,2,*, AZIZAH MISRAN², MASHITAH JUSOH², YUSUFF OLADOSU¹, FATAI AROLU¹, AZADUL HAQUE¹, NADZIRAH M. SULAIMAN¹

BIOCELL, Vol.45, No.5, pp. 1393-1401, 2021, DOI:10.32604/biocell.2021.015321 - 12 July 2021

Abstract Indo-Burmese region was the primary center of eggplant diversity from where the crop extended to several secondary origins of diversity. In this study, the genetic diversity among fifty-six eggplant accessions collected from three countries was assessed using sixteen polymorphic SSR markers to determine suitable parents for heterotic hybridization. The estimation of genetic diversity among the population of three countries (Bangladesh, Malaysia, and Thailand) varied from 0.57 to 0.74, with Shannon’s index value of 0.65. The mean value of expected heterozygosity and Nei’s index was 0.49, with an average PIC value of 0.83. A dendrogram was… More >

Riaz Ahmad¹, Muhammad Akbar Anjum^1,*, Safina Naz¹, Rashad Mukhtar Balal²

Phyton-International Journal of Experimental Botany, Vol.90, No.1, pp. 17-34, 2021, DOI:10.32604/phyton.2020.011680 - 20 November 2020

Abstract Selection and use of molecular markers for evaluation of DNA polymorphism in plants are couple of the most important approaches in the field of molecular genetics. The assessment of genetic diversity using morphological markers is not sufficient due to little differentiating traits among the species, genera or their individuals. Morphological markers are not only highly influenced by environmental factors but skilled assessment is also prerequisite to find the variations in plant genetic resources. Therefore, molecular markers are considered as efficient tools for detailed DNA based characterization of fruit crops. Molecular markers provide new directions to More >

Ning Xuan¹, Hua Zhang¹, Xu Liu¹, Qiang Guo¹, Jian Liu², Fangyin Yao^1,*

Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 45-55, 2020, DOI:10.32604/phyton.2020.08548 - 01 March 2020

Abstract Rice blast disease is one of the most devastating diseases of rice (Oryza sativa L.) caused by the fungus Magnaporthe oryzae (M. oryzae), and neck blast is the most destructive phase of this illness. The underlying molecular mechanisms of rice blast resistance are not well known. Thus, we collected 150 rice varieties from different ecotypes in China and assessed the rice blast resistances under the natural conditions that favoured disease development in Jining, Shandong Province, China in 2017. Results showed that 92 (61.3%) and 58 (38.7%) rice varieties were resistant and susceptible to M. oryzae, respectively. Among the 150… More >