Rui Zhang¹, Taixiang Chen¹, Zhenjiang Chen¹, Hao Chen¹, Xuekai Wei¹, Malik Kamran¹, Chunjie Li^1,2,*

Phyton-International Journal of Experimental Botany, Vol.93, No.3, pp. 567-580, 2024, DOI:10.32604/phyton.2024.047485

Abstract In a greenhouse experiment, the effects of soil microorganisms and extracts of Achnatherum inebrians on the seed germination and seedling growth of Elymus nutans were studied. The results showed that both the extracts from aboveground and belowground parts of A. inebrians significantly inhibited the germination rate, germination potential, germination index, vigor index, seedling height, root length, and fresh weight of E. nutans, but increased malondialdehyde content, catalase, peroxidase and superoxide dismutase activity of E. nutans seedlings (p < 0.05). The allelopathy of aqueous extracts of the aboveground parts of A. inebrians was stronger than that of the precipitates. Aqueous extracts… More >

Genhai Hu^1,*, Maoni Chao¹, Xiuren Zhou², Yuanzhi Fu²

Phyton-International Journal of Experimental Botany, Vol.92, No.9, pp. 2535-2554, 2023, DOI:10.32604/phyton.2023.030163

Abstract Gossypium hirsutum L. is an important cash crop native to the subtropics and is widely cultivated around the world. Low temperature is an important stress that seriously affects seed germination and emergence during planting. In this study, transcriptomic profiles of low-temperature- and normal-temperature-germinated seeds of Xinluzao 25, a variety with low-temperature tolerance and high germination rates, were analyzed and compared. The following results were obtained. (1) A total of 81.06 Gb of clean data were obtained after transcriptome sequencing and assembly, and 76,931 non-redundant Unigene sequences were obtained after data consolidation and concatenation; of these, 69,883 Unigene sequences were annotated.… More >

Yuquan Huang^1,2, Fan Wei¹, Qing Ma², Yang Lin¹, Jinpeng Huang², Yanxia Zhu^1,*, Danfeng Tang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2105-2116, 2023, DOI:10.32604/phyton.2023.029205

Abstract Ilex asprella (Hook. et Arn.) Champ. ex Benth is one of the most important traditional Chinese medicines in southern China. The seeds of Ilex asprella usually have extremely low germination due to their dormancy characteristics, which severely impacts the efficiency of seedling raising and increases labor costs. In this study, to improve the seed germination of I. asprella, the effects of germination substrate, hormone, winnowing, and stratification treatments on the seed germination of I. asprella were investigated. The results of the germination matrix showed that the highest germination percentage of 45.2% was achieved under the 20°C/10°C day/night temperature and vermiculite… More >

Cong Hou¹, Kang Ning¹, Xiuye Wei¹, Yufei Cheng¹, Huatao Yu¹, Haibin Yu², Xia Liu^1,*, Linlin Dong^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.7, pp. 2087-2103, 2023, DOI:10.32604/phyton.2023.029041

Abstract Abscisic acid (ABA) is involved in regulating diverse biological processes, but its signal transduction genes and roles in hemp seed germination are not well known. Here, the ABA signaling pathway members, PYL, PP2C and SnRK2 gene families, were identified from the hemp reference genome, including 7 CsPYL (pyrab-actin resistance1-like, ABA receptor), 8 CsPP2CA (group A protein phosphatase 2c), and 7 CsSnRK2 (sucrose nonfermenting1-related protein kinase 2). The content of ABA in hemp seeds in germination stage is lower than that in non-germination stage. Exogenous ABA (1 or 10 μM) treatment had a significant regulatory effect on the selected PYL, PP2C,… More >

Yue Wang^1,#, Xiujie Li^2,#, Yusen Wu², Zhonghui Cai¹, Bo Li², Zhaosen Xie^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1859-1871, 2023, DOI:10.32604/phyton.2023.027067

Abstract It is generally agreed that many Vitis vinifera L. cultivars are self-fertile, where self-pollination often occurs before capfall in a process called cleistogamy. Therefore, it is difficult to identify the right time to remove stamens before self-pollination during the cross-breeding of grape. For this paper, we observed the process of grape flowering and measured the pollen viability and stigma receptivity of grape flowers of ‘Shine Muscat’ in order to identify the starting time of self-pollination before capfall and to provide useful information for improving the efficiency of cross-breeding. The results demonstrate that the anther is not cracked during the visible… More >

Adalberto Benavides-Mendoza¹, Rebeca Betancourt-Galindo², Nazario Francisco-Francisco^3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1831-1840, 2023, DOI:10.32604/phyton.2023.028085

Abstract

Micronutrient nanoparticles (NPs) are currently an option for chemical fertilization and biostimulation in crops. However, there is little information on the phytotoxic or biostimulatory effects of NPs at low concentrations of some elements, such as Zn. In this study, the effect of low concentrations of Zn oxide (ZnO) NPs on germination, growth variables, and nutritional attributes of lettuce (Lactuca sativa L.) was evaluated in comparison to Zn sulfate. Romaine lettuce seeds were treated with ZnSO₄^-- × 7H₂O and ZnO NPs at Zn molar concentrations of 1 × 10⁻³, 5 × 10⁻³, 1 × 10⁻⁴, 5 × 10⁻⁴, 1… More >

Yanyan Pu^1,#, Liwen Wang^2,#, Leilei Li³, Yujun Si⁴, Shubin Xie², Yunzhe Cong¹, Dong Wang¹, Yongchao Gong¹, Rumei Tian¹, Xue Chen³, Xiaoyan Zhang¹, Min Liu¹, Hanfeng Ding^1,3,*, Nana Li^1,3,*

Phyton-International Journal of Experimental Botany, Vol.92, No.6, pp. 1793-1812, 2023, DOI:10.32604/phyton.2023.026822

Abstract During the life cycle of a plant, seed germination is crucial. Upon ingestion of water, the dry seeds resumed energy metabolism and cellular repair. To dissect the complex mechanisms at the very beginning of seed germination, two approaches including transcriptome and small RNA sequencing were conducted during the water imbibition process of mung bean seeds compared with dry seed. The transcriptome sequencing analysis identified 10,108 differentially expressed genes (DEGs) between dry and imbibed mung bean seeds. KEGG enrichment analyses demonstrated numerous DEGs involved in hormone signaling pathways, carbohydrate, and energy metabolism. Out of the total DEGs, 129 genes were investigated… More >

Luqian Zhang^1,#, Xilin Fang^1,#, Nan Yu¹, Jun Chen¹, Haodong Wang¹, Quansheng Shen¹, Guanghui Chen^2,*, Yue Wang^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.5, pp. 1571-1587, 2023, DOI:10.32604/phyton.2023.025481

Abstract Drought stress is a serious threat to the germination of plant seeds and the growth of seedlings. Melatonin has been proven to play an important role in alleviating plant stress. However, its effect on seed germination under drought conditions is still poorly understood. Therefore, we studied the effects of melatonin on rice seed germination and physiological characteristics under drought stress. Rice seeds were treated with different concentrations of melatonin (i.e., 0, 20, 100, and 500 μM) and drought stress was simulated with 5% polyethylene glycol 6000 (PEG6000). The results showed that 100 μM melatonin can effectively improve the germination potential,… More >

Sasa Jing¹, Yuan Tian¹, Heng Zhang², John T. Hancock³, Ying Zhu^2,*, Ping Li^1,*

Phyton-International Journal of Experimental Botany, Vol.92, No.4, pp. 1203-1214, 2023, DOI:10.32604/phyton.2023.026305

Abstract The crop seeds have been a staple food for humans, and seed yield is important for sustaining agriculture development and enhancing human adaptability to food risks. The phenomenon of pre-harvest sprouting (PHS), caused by seed dormancy deficiency, and the phenomenon of low seedling emergence caused by seed deep dormancy, will lead to a reduction in agricultural production. Therefore, it is particularly important to understand the regulation mechanisms of seed dormancy. There are many studies on the regulation of seed dormancy in rice, but there are few studies on the regulation of seed dormancy in other crops, and the research on… More >

Walid Soufan^*, Yaser Hassan Dewir, Nasser A. Al-Suhaibani

Phyton-International Journal of Experimental Botany, Vol.92, No.1, pp. 111-120, 2023, DOI:10.32604/phyton.2022.023115

Abstract Alfalfa (Medicago sativa L.), when exposed to abiotic stress such as salinity, suffers significant losses in yield and productivity. The present study evaluated the salinity tolerance of 12 alfalfa cultivars in vitro using five concentrations of sodium chloride (NaCl), ranging from 0 to 250 mmol L⁻¹ . The results obtained in the current study revealed that the Saudi cultivars, Kasimi and Hassawi, and the German cultivar (Berlin) had the highest salinity tolerance in terms of germination percentage (GP), corrected germination rate index (CGRI), days to reach 50% germination (GT50), and ability to form cotyledonary and true leaves. Under mmol L⁻¹… More >