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    ARTICLE

    Optimization of Callus Induction Conditions from Immature Embryos in Maize and Plant Regeneration

    Peng Jiao1,#, Ruiqi Ma2,#, Zhuo Qi1, Zhenzhong Jiang1, Siyan Liu1, Jing Qu3, Shuyan Guan1,*, Yiyong Ma3,*

    Phyton-International Journal of Experimental Botany, Vol.89, No.1, pp. 121-130, 2020, DOI:10.32604/phyton.2020.07980 - 01 March 2020

    Abstract This research uses the immature embryos of inbred maize lines (GSH9901, Hi01, Hi02, and Chang 7-2) as receptor materials to establish the callus induction system. These inbred lines provide the receptor materials for the genetic regeneration of maize and the verification of the genetic functions of maize. The factor experiment and orthogonal experiments were used to investigate the impacts of different genotypes, immature embryo size, shield orientation, 2, 4-D concentration, proline concentration, and folic acid concentration on the induction rate of embryogenic callus tissue. A sensitivity experiment testing glyphosate (Bar) and an antibiotic (Cefotaxime sodium)… More >

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